Detection of Reactive Oxygen Species by a Carbon-Dot–Ascorbic Acid Hydrogel

Abstract

Detection of reactive oxygen species (ROS) is important in varied biological processes, disease diagnostics, and chemotherapeutic drug screening. We constructed a ROS sensor comprising an ascorbic-acid-based hydrogel encapsulating luminescent amphiphilic carbon-dots (C-dots). The sensing mechanism is based upon ROS-induced oxidation of the ascorbic acid units within the hydrogel scaffold; as a consequence, the hydrogel framework collapses resulting in aggregation of the C-dots and quenching of their luminescence. The C-dot-hydrogel platform exhibits high sensitivity and detected ROS generated chemically in solution and in actual cell environments. We demonstrate application of the C-dot-hydrogel for evaluating the efficacy of a chemotherapeutic substance, underscoring the potential of the system for drug screening applications.

Hydrogel-Based Technologies for the Diagnosis of Skin Pathology

“…In Figure 5C, Bhattacharya et al demonstrated an ascorbic acid gel encapsulating luminescent carbon dots for ROS detection. In the presence of ROS moieties, oxidation of the gel framework leads to aggregation of carbon dots and hence quenched luminescence [115].…”

Reactive oxygen species in photodynamic therapy

“…光素(H 2 -DCF)的细胞内ROS检测的优异探针, 其氧 化产物钙黄绿素不会泄漏出细胞, 积累在线粒体中, 而二氢荧光素位于细胞质中, H 2 -Calcein的氧化可能 是电子直接转移到线粒体复合物I的结果 [110] . Jelinek 课题组 [111] [111] .…”

Extensive Study of Rhodanine‐Arylamine‐Based Chromophores: Consolidated Optical, DFT/TD‐DFT and Non‐Linear Optical Properties

“…The fluorescent D‐π‐A compounds with ‐COOH group are important for NIR probes in the detection of living cell and in vivo imaging, flow cytometry, and reactive oxygen species sensors,, They are used in bio‐conjugation with affinity proteins and antibodies,, tagging quantum dots (QD) for resonance energy transfer, and carbon quantum dots for DNA sensing . Rhodanine‐3‐acetic acid (RAA) is a widely used acceptor in DSSCs ,.…”