Detection of recent hybridization between sympatric Chilean Puya species (Bromeliaceae) using AFLP markers and reconstruction of complex relationships

Schulte, Katharina; Silvestro, Daniele; Kiehlmann, Elke; Vesely, Sanja; Novoa, Patricio; Zizka, Georg

doi:10.1016/j.ympev.2010.09.001

Cited by 59 publications

(71 citation statements)

References 57 publications

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“…While most accessions have a very high probability (usually around 0.9) to belong to one cluster, hybrid individuals tend to have a much lower probability (< 0.5) and have a, often only slightly lower, probability to belong to another cluster. Schulte et al [43] also argue that a posterior probability lower than 0.5 provides strong evidence for a recent hybrid origin of individuals.…”

Section: Discussionmentioning

confidence: 99%

What's in a name; Genetic structure in Solanum section Petota studied using population-genetic tools

et al. 2011

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BackgroundThe taxonomy and systematic relationships among species of Solanum section Petota are complicated and the section seems overclassified. Many of the presumed (sub)species from South America are very similar and they are able to exchange genetic material. We applied a population genetic approach to evaluate support for subgroups within this material, using AFLP data. Our approach is based on the following assumptions: (i) accessions that may exchange genetic material can be analyzed as if they are part of one gene pool, and (ii) genetic differentiation among species is expected to be higher than within species.ResultsA dataset of 566 South-American accessions (encompassing 89 species and subspecies) was analyzed in two steps. First, with the program STRUCTURE 2.2 in an 'unsupervised' procedure, individual accessions were assigned to inferred clusters based on genetic similarity. The results showed that the South American members of section Petota could be arranged in 16 clusters of various size and composition. Next, the accessions within the clusters were grouped by maximizing the partitioning of genetic diversity among subgroups (i.e., maximizing Fst values) for all available individuals of the accessions (2767 genotypes). This two-step approach produced an optimal partitioning into 44 groups.Some of the species clustered as genetically distinct groups, either on their own, or combined with one or more other species. However, accessions of other species were distributed over more than one cluster, and did not form genetically distinct units.ConclusionsWe could not find any support for 43 species (almost half of our dataset). For 28 species some level of support could be found varying from good to weak. For 18 species no conclusions could be drawn as the number of accessions included in our dataset was too low. These molecular data should be combined with data from morphological surveys, with geographical distribution data, and with information from crossing experiments to identify natural units at the species level. However, the data do indicate which taxa or combinations of taxa are clearly supported by a distinct set of molecular marker data, leaving other taxa unsupported. Therefore, the approach taken provides a general method to evaluate the taxonomic system in any species complex for which molecular data are available.

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Section: Discussionmentioning

confidence: 99%

What's in a name; Genetic structure in Solanum section Petota studied using population-genetic tools

et al. 2011

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“…Hypochaeris , [15]; Lactuca , [16]; Phylica , [17]; Trollius , [18]; Ranunculus alpestris , [19]; Puya , [20,21]; Araucaria , [22]). In contrast to standard phylogenetic markers, AFLP variation is spread across the whole genome, spanning both coding and non-coding DNA regions and may therefore be more representative of overall genetic patterns present as well as being highly informative for phylogenetic analyses at the low phylogenetic level [23,24].…”

Section: Introductionmentioning

confidence: 99%

Analyses of amplified fragment length polymorphisms (AFLP) indicate rapid radiation of Diospyros species (Ebenaceae) endemic to New Caledonia

et al. 2013

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BackgroundRadiation in some plant groups has occurred on islands and due to the characteristic rapid pace of phenotypic evolution, standard molecular markers often provide insufficient variation for phylogenetic reconstruction. To resolve relationships within a clade of 21 closely related New Caledonian Diospyros species and evaluate species boundaries we analysed genome-wide DNA variation via amplified fragment length polymorphisms (AFLP).ResultsA neighbour-joining (NJ) dendrogram based on Dice distances shows all species except D. minimifolia, D. parviflora and D. vieillardii to form unique clusters of genetically similar accessions. However, there was little variation between these species clusters, resulting in unresolved species relationships and a star-like general NJ topology. Correspondingly, analyses of molecular variance showed more variation within species than between them. A Bayesian analysis with BEAST produced a similar result. Another Bayesian method, this time a clustering method, Structure, demonstrated the presence of two groups, highly congruent with those observed in a principal coordinate analysis (PCO). Molecular divergence between the two groups is low and does not correspond to any hypothesised taxonomic, ecological or geographical patterns.ConclusionsWe hypothesise that such a pattern could have been produced by rapid and complex evolution involving a widespread progenitor for which an initial split into two groups was followed by subsequent fragmentation into many diverging populations, which was followed by range expansion of then divergent entities. Overall, this process resulted in an opportunistic pattern of phenotypic diversification. The time since divergence was probably insufficient for some species to become genetically well-differentiated, resulting in progenitor/derivative relationships being exhibited in a few cases. In other cases, our analyses may have revealed evidence for the existence of cryptic species, for which more study of morphology and ecology are now required.

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“…The detection of hybrids within Bromeliaceae can, however, be difficult based on morphological characters alone, as was demonstrated in a study on Puya, where chloroplast capture and secondary hybridization were observed using plastid and nuclear sequence data (Jabaily and Sytsma 2010). With special focus on Chilean Puya species, Schulte et al (2010) were able to detect recent hybrids and introgression using AFLP data. The latter authors assumed that efficient reproductive barriers may establish only slowly, permitting hybridization Fig.…”

Section: Hybridization and Reproductive Barriers In Bromeliaceaementioning

confidence: 99%

Reproduction biology and chloroplast inheritance in Bromeliaceae: a case study in Fosterella (Pitcairnioideae)

et al. 2015

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We applied a series of intra-and interspecific in situ cross-pollination experiments under greenhouse conditions to evaluate the breeding systems in four Fosterella species (Pitcairnioideae s.str.; Bromeliaceae). Viable hybrids were produced between each pair of the investigated species, suggesting that reproduction barriers may be low also under natural conditions. Seed germination rates proved to be high in each crossing treatment, indicating a high viability of the artificial hybrids. Large numbers of seeds were produced after both closed and open pollination treatments, suggesting that autogamy may be a major reproductive strategy in the genus. Our results support the concept that self-compatibility is an appropriate way to avoid natural hybridization in Bromeliaceae and could assist in maintaining species integrity in the presence of pollen flow. Paternity was verified in all crosses by genotyping parents and offspring with a set of polymorphic nuclear microsatellite markers. To study the mode of chloroplast inheritance, we developed a novel set of 24 chloroplast microsatellite markers using 454 pyrosequencing technology, and applied four of these markers for genotyping parents and offspring from all crosses. Our results clearly demonstrated a maternal inheritance of plastids.

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Detection of recent hybridization between sympatric Chilean Puya species (Bromeliaceae) using AFLP markers and reconstruction of complex relationships

Cited by 59 publications

References 57 publications

What's in a name; Genetic structure in Solanum section Petota studied using population-genetic tools

What's in a name; Genetic structure in Solanum section Petota studied using population-genetic tools

Analyses of amplified fragment length polymorphisms (AFLP) indicate rapid radiation of Diospyros species (Ebenaceae) endemic to New Caledonia

Reproduction biology and chloroplast inheritance in Bromeliaceae: a case study in Fosterella (Pitcairnioideae)

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