Detection of resistance loci to English grain aphid in common wheat

Abstract

The English grain aphid (Sitobion avenae F.) causes significant yield and quality losses in wheat (Triticum aestivum L.) worldwide, and the use of insect‐resistant wheat cultivars reduces the loss level. The present study aimed to identify quantitative trait loci (QTL) and perform putative candidate gene evolutionary analysis for English grain aphid resistance in 261 F2:5 recombinant inbred lines (RILs) derived from the cross Amigo/XN1376. The RIL population and parents were genotyped with the Wheat 55K single nucleotide polymorphism (SNP) array. A total of 14,694 polymorphic SNPs were used to construct a genetic linkage map. One major effect of QTL was detected in F2:5 RILs and was located on chromosome 3DL, explained 19.33% phenotypic variance, temporarily designated as QSa3.haust‐3D. It was located in a 2.73 Mb, flanked by markers AX‐109429351 and AX‐110834100, from 550.25 to 552.98 Mb according to Chinese Spring reference genome. This region contained 39 annotated genes. Eight putative candidate genes for resistance to English grain aphid were identified among these genes. The QTL with closely linked SNP markers could be useful for marker‐assisted selection for resistance to English grain aphid for wheat breeding.

Identification of Candidate Genes for English Grain Aphid Resistance from QTLs Using a RIL Population in Wheat

“…Luo et al [26] found five potential candidate genes, which are different from our study. QSa3.haust-3D included eight putative candidate genes, TraesCS3D02G440100, TraesCS3D02G440300, TraesCS3D02G440400, TraesCS3D02G440800, TraesCS3D02G441200, TraesCS3D02G441300, TraesCS3D02G441900 and TraesCS3D02G443500 [35]. We identified three genes TraesCS3D02G075600, TraesCS-3D02G085400LC and TraesCS3D02G087600 on 3D, which are different from the candidate genes of QSa3.haust-3D.…”

“…The TL-RIL population and its parents were planted in a single hole with a spacing of 30 × 30 cm, 10 seeds per hole, five plants reserved at the seedling stage, and two replications in two growing seasons 2019-2020 and 2020-2021. The number of aphids was investigated under natural infestation, with three measured times (MTs) in each year, every 7 days in the jointing stage [35]: 23 April (20MT1), 30 April (20MT2) and 7 May (20MT3) in 2020; and 21 April (21MT1), 28 April (21MT2) and 5 May (21MT3) in 2021. For each replication, the number of EGAs on 20 tillers was measured for each line.…”

“…Some QTLs and/or candidate genes associated with EGA resistance have also been identified. Zeng et al [35] constructed a genetic linkage map with a total of 14,694 polymorphic SNPs of a RIL population. One major QTL, QSa3.haust-3D, was identified for EGA resistance on 3DL between 550.25 and 552.98 Mb in Chinese Spring (CS) RefSeq v1.1 [36], and eight putative candidate genes were identified from this region [36].…”

Integrated genome‐wide association study and QTL mapping reveals qSa‐3A associated with English grain aphid, Sitobion avenae (Fabricius) resistance in wheat

“…27 By counting the number of S. avenae in tillers under natural infestation, Wang et al reported Sa1 and Sa2 genes for S. avenae resistance on chromosome 7D, 3,17 and Zeng et al identified QSa.haust-3D for S. avenae resistance on chromosome 3D. 28 Liu et al found S. avenae resistance gene RA-1 on chromosome 6AL using artificial inoculation in the field and greenhouse. 29 Liu et al detected 18 SSR markers associated with S. avenae resistance on chromosomes 1A, 1B, 3A, 3B, 3D, 4A, 4B, 5B, 5D, 6B and 7A in a natural population through association study.…”