Detection of Rhyzopertha dominica larvae in stored wheat using ELISA: The impact of myosin degradation following fumigation

Atui, Márcia Bittar; Flinn, Paul W.; Lázzari, Sonia Maria Noemberg; Lazzari, F. A.

doi:10.1016/j.jspr.2006.04.001

Cited by 19 publications

(3 citation statements)

References 8 publications

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“…Quinn et al (30) described an immunochemical assay with antimyosin Pabs that allowed the measurement of insect contamination at the level corresponded to 28 ng of myosin/ mL. Atui et al (6) adopted this assay to detect the internalfeeding species Rhyzopertha dominica. They reliably detected 10 insects per kg.…”

Section: Discussionmentioning

confidence: 99%

Development of Polyclonal Antibodies for the Detection of Tribolium castaneum Contamination in Wheat Grain

Krizkova-Kudlikova

Hubert

2008

J. Agric. Food Chem.

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Two polyclonal antibodies (Pab) were developed for the detection of Tribolium castaneum, which is a stored product pest of medical and economical importance. Selected Pab anti- T. castaneumK51 showed low cross-reactivity to other stored product arthropods but revealed high reactivity to T. destructor, T. confusum, and partly to Tenebrio molitor. PTA-ELISA was used to detect adults, larva, and feces of T. castaneum in artificially contaminated grain samples. Calibration methods were applied to determine detection limits for each type of contaminants. Anti- T.castaneumK51 enabled detection of T. castaneum in grain samples; detection limits reached 60 and 640 individuals/kg of grain for larvae and adults, respectively, and 4 mg of feces/kg of grain. After recalculation, the detection limit for feces enables detection of 30 larvae after 5 days of feeding in optimal conditions. The main advantage of the developed assay is traceability of T. castaneum contamination, especially when the adults and larvae are removed from contaminated material, based on the detection of feces that persist in the grain.

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Section: Discussionmentioning

confidence: 99%

Development of Polyclonal Antibodies for the Detection of Tribolium castaneum Contamination in Wheat Grain

Krizkova-Kudlikova

Hubert

2008

J. Agric. Food Chem.

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“…The standard ßotation method requires a continuous supply of laboratory chemicals (i.e., hydrochloric acid, mineral oil, and isopropanol), whereas the ELISA procedure also requires purchase of disposable kits and reagents for each sample. The ELISA method to detect myosin also has several technical issues that impact its accuracy: there are variable amounts of myosin in different life stages (Schatzki et al 1993) and myosin degrades during the Þrst 2 wk after insect death, but it remains relatively stable after that initial decay period (Atui et al 2007). After the calibration model is developed, NIRS determination does not require highly skilled labor for operation.…”

Section: Discussionmentioning

confidence: 99%

Rapid Assessment of Insect Fragments in Flour Milled from Wheat Infested with Known Densities of Immature and Adult <I>Sitophilus oryzae</I> (Coleoptera: Curculionidae)

Toews¹,

Perez-Mendoza²,

Throne³

et al. 2007

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Milling wheat, Triticum aestivum L., infested with low densities of internal feeding insects can result in flour containing insect fragments. The Food and Drug Administration (FDA) enforces a standard or defect action level stating that a maximum of 75 insect fragments per 50 g of flour is allowed. However, the relationship between level of infestation and number of resulting fragments is not well documented, and a more rapid method for enumerating insect fragments is needed. We characterized the number of insect fragments produced from milling small lots of wheat spiked with known densities and life stages of Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Insect fragments were enumerated with near-infrared spectroscopy (NIRS), a quick nondestructive procedure, and with the industry standard flotation method. Results showed that an individual small larva, large larva, pupa, or adult produced 0.4, 0.7, 1.5, and 27.0 fragments, respectively. NIRS-predicted counts of < or =51 (from small larvae), < or =53 (from large larvae), < or =43 (from pupae), or 0 (from adults) indicated that there were <75 actual fragments in that sample, because the upper bound of associated 95% inverse prediction confidence intervals was less than the standard; NIRS-predicted counts of > or =98, > or =117, 108, or > or =225 fragments (same life stages as above) signaled that these flour samples contained >75 actual fragments. These data suggest that NIRS could be adopted for rapid assessment of insect fragments resulting from relatively low levels of infestation with immature life states, but that it was not accurate enough for enumerating insect fragments, relevant to FDA standards, resulting from adults.

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“…However, insects are often hidden inside the kernel and are not detected by sieving. To detect both hidden and external insects, the following methods have been developed: flotation and cracking (Brader et al, 2002), acoustic sensor (Gutierez et al, 2010), immunoassay (Krizkova-Kudlikova and Hubert, 2008;Atui et al, 2007), single kernel characterization (SKCS) (Pearson et al, 2003), electrical conductance (Brabec et al, 2010;Pearson and Brabec, 2007), near-infrared hyperspectral imaging (Singh et al, 2009), solid-phase micro extraction (SPME) (Laopongsit et al, 2014), thermal camera imaging (Manickavasagan et al, 2008), and soft eX ray roentgenography (Karunakaran et al, 2004;Nawrocka et al, 2012). Most of these methods are not commercialized or used by the grain industry due to one or some of the following disadvantages: high cost, limited capacity, intensive labour, time consuming, safety issue, and low accuracy.…”

Section: Introductionmentioning

confidence: 99%

A new method to rapidly detect rusty grain beetle, Cryptolestes ferrugineus (Stephens), in stored grain

Jian

Jayas

Fields

et al. 2015

Journal of Stored Products Research

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Detection of Rhyzopertha dominica larvae in stored wheat using ELISA: The impact of myosin degradation following fumigation

Cited by 19 publications

References 8 publications

Development of Polyclonal Antibodies for the Detection of Tribolium castaneum Contamination in Wheat Grain

Development of Polyclonal Antibodies for the Detection of Tribolium castaneum Contamination in Wheat Grain

Rapid Assessment of Insect Fragments in Flour Milled from Wheat Infested with Known Densities of Immature and Adult <I>Sitophilus oryzae</I> (Coleoptera: Curculionidae)

A new method to rapidly detect rusty grain beetle, Cryptolestes ferrugineus (Stephens), in stored grain

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