Detection of Riemerellosis in Ducks by gyrB Gene Based Polymerase Chain Reaction

Udayan, Parvathy; Mani, Priya S.; Kollerate, Siniya; Balan, Rinsha; Mangottumuruppel, Mini

doi:10.5455/ijlr.20180714070806

Cited by 5 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a comparative study, Wang et al, (2012) could confirm gyrB-based PCR to be more consistent and was more specific (100.0%) for detection of R. anatipestifer strains in comparison to the 16S rRNA based PCR (63.6%). In similar comparison with a pair of PCR primers designed for gyrB gene sequence of R. anatipestifer, Udayan et al, (2019) concluded the gyrB-based PCR to be more accurate than 16S rRNA sequence-based PCR with confirmation of R. anatipestifer in a record 93.3% of throat swabs. They also opined for a faster evolutionary rate of the gyrB gene as main criteria behind its selection and application.…”

Section: Molecular Detection Of Gyrb Genementioning

confidence: 84%

“…clinical samples from birds. In a recent study, Udayan et al, (2019) reported the universal gyrase B-encoding gene (gyrB), a type II DNA topoisomerase as a more accurate, sensitive and specific marker for R. anatipestifer identification. R. anatipestifer infection can be treated and controlled by judicious use of suitable antibiotics.…”

Section: Issn: 2319-7706 Volume 9 Number 3 (2020)mentioning

confidence: 99%

“…Extracted DNA of respective R. anatipestifer isolates were further tested for the presence of a gyrBgene (162 bp) by simplex PCR, as mentioned by Udayan et al, (2019) with slight modifications. Details of primers sequences for the gyrB gene are tabulated in Table 1.…”

Section: Molecular Detection Of Gyrb Genementioning

confidence: 99%

See 2 more Smart Citations

Isolation and Molecular Identification of Riemerella anatipestifer from Ducks in Assam

Hazarika¹,

Doley²,

Sarmah³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Section: Molecular Detection Of Gyrb Genementioning

confidence: 84%

Section: Issn: 2319-7706 Volume 9 Number 3 (2020)mentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Molecular Identification of Riemerella anatipestifer from Ducks in Assam

Hazarika¹,

Doley²,

Sarmah³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…In this study, we identi ed the pathogenic organism based on species speci c gene, gyrase B-encoding gene (gyrB), gene and groEL gene and moreover studied antibiogram of R. anatipestifer through Kirby Bauer disc diffusion as a very quick and cost-effective method to reduce the mortality in the ock. The universal gyrB, a type II DNA topoisomerase, is thought to be a more accurate, sensitive, and speci c marker for identifying R. anatipestifer stated in recent study (Udayan et al 2019). On the other hand, the groEL gene which has several highly conserved regions, explored as reliable molecular marker for bacterial genus and species identi cation (Nishibuchi 2006;Hossain et al 2012).…”

Section: Introductionmentioning

confidence: 96%

“…Though no signi cant cross-protection has been reported against R. anatipestifer infection (Higgins et al 2000), regular vaccination along with duck plague and duck pasteurellosis may reduce the incidence. Interestingly, R. anatipestifer has greater phenotypic similarities with Pasteurella multocida and due to the similarities of both morphologically and culturally, it is sometimes di cult to isolate the organism through conventional methods in the laboratory and furthermore, to the eld veterinarians (Kardos et al 2007; Udayan et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and antibiogram profile of Riemerella anatipestifer in duck farming areas of Bangladesh

Hasan

Bose

Aktar

et al. 2022

Preprint

View full text Add to dashboard Cite

Riemerella anatipestifer is a Gram-negative, cocco-bacillary, non-motile, bipolar, nonsporulating bacterium, belong to the family Weeksellaceae and order Flavobacteriales. The bacterium causes the great economic losses in duck farming areas every year due to miss identification with other co-infection. Therefore, a study was designed to detect R. anatipestifer at molecular level through polymerase chain reaction (PCR) from ducks of Mymensingh division and Sylhet division and to determine the antibiogram profile of the PCR positive isolates using disc diffusion method. A total of 52 samples were collected, comprising clinically sick (32) and dead ducks (20). PCR confirmation was accomplished and consistent findings were observed, employing R. anatipestifer specific PCR assay (546 bp), gyrB-based PCR (162 bp), as well as groEL (271 bp) gene as appropriate molecular markers. A total 21 samples, 8 from clinically sick birds and 13 from dead showed the positive results both conventional and at molecular assay out of 52 samples. The oropharyngeal swab from sick ducks, liver and heart from dead ducks revealed high prevalence rate of positive isolates. Antibiotic susceptibility test revealed that the isolates were 100% resistant against Beta-lactams (Penicillin G, Cephradin), Aminoglycosides (Streptomycin, Neomycin, and Gentamycin), Penems (Meropenem), and Macrolids (Erythromycin), however, 100% sensitive to Sulphonamides (Cotrimoxazol), Phenicols (Florfenicol), and Quinolones (Levofloxacin).

show abstract

An overview on riemerellosis: A worldwide emerging disease of ducks

Abd El-Ghany

2023

BJVM

View full text Add to dashboard Cite

Riemerella anatipestifer (R. anatipestifer) is the bacterial cause of an economically important and serious disease of ducks and other poultry species. Extensive reports showed that this disease condition is widely distributed in different countries since 1904. Horizontal and mechanical transmissions are important routes of R. anatipestifer dissemination. The disease is characterised by respiratory, nervous and locomotor disturbance with high mortality rates especially in ducklings. Affected birds showed generalised polyserositis or localised lesions in different organs. The clinical picture of R. anatipestifer is similar to and confused with other bacterial infections, so diagnosis of the disease relies mainly on laboratory techniques. At least 21 serotypes of R. anatipestifer have been identified. Control of R. anatipestifer infection mainly depends on using of the suitable antibiotics according to the antibiogram results. Due to the extensive and hazardous uses of antibiotics, development of multi-drug resistance strains of R. anatipestifer is common. Prevention of the disease can be achieved though application of good management practice and vaccination. Different types of vaccines are commercially available. There are autogenous polyvalent live or inactivated bacterins as well as subunit and recombinant vaccines. The vaccines give protection only for the specific serotypes present in the used vaccines. Therefore, this review article gives an overview on R. anatipestifer infections regarding the distribution all over the world, susceptibility and infection, clinical picture, laboratory diagnosis as well as prevention and control methods.

show abstract

Detection of Riemerellosis in Ducks by gyrB Gene Based Polymerase Chain Reaction

Cited by 5 publications

References 0 publications

Isolation and Molecular Identification of Riemerella anatipestifer from Ducks in Assam

Isolation and Molecular Identification of Riemerella anatipestifer from Ducks in Assam

Molecular detection and antibiogram profile of Riemerella anatipestifer in duck farming areas of Bangladesh

An overview on riemerellosis: A worldwide emerging disease of ducks

Contact Info

Product

Resources

About