Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis

The epidemiology, pathogenesis, transmission, diagnosis, and management of multidrug-resistant, extensively drug-resistant, and incurable tuberculosis

“…244 Resistance to rifampicin is almost entirely due to changes in the β subunit of DNA-dependent RNA polymerase, which is encoded by the rpoB gene. 245 Molecular tests targeting this gene are more accurate than using phenotypic DST. 241 However, caution must be used when interpreting data from some molecular tests because not all mutations have the same effect.…”

Insights into the processes that drive the evolution of drug resistance in Mycobacterium tuberculosis

“…Despite the large diversity of mutation patterns globally, only few specific mutations are predominant (Table 2) (Sandgren et al., 2009; Zhang & Yew, 2015). For instance, in the case of rifampicin resistance, hundreds of rpo B mutations have been described (not all were associated with rifampicin resistance), but more than 80% of rifampicin‐resistant isolates display mutations in three codons rpo B531, 526 and 516 (Campbell et al., 2011; Lipin et al., 2007; Pozzi et al., 1999; Telenti et al., 1993). Similar to that, among the approximately 300 mutations found in the kat G gene, the prevalence of kat G S315T mutation can vary between 32% and 95% in isoniazid‐resistant clinical isolates depending on the geographic regions and drug resistance patterns (Hazbon et al., 2006; Lipin et al., 2007; Mokrousov et al., 2002; Vilcheze & Jacobs, 2014).…”

“… Notes aNucleotide change.bSee the following studies for reference (Campbell et al., 2011; Duong et al., 2009; Hazbon et al., 2006; Hillemann et al., 2005; Lipin et al., 2007; Mokrousov et al., 2002; Müller et al., 2011; Niehaus et al., 2015; Perdigao et al., 2010; Pozzi et al., 1999; Qian et al., 2002; Shi et al., 2011; van Soolingen et al., 2000; Sreevatsan et al., 1996, 1997; Telenti et al., 1993; Von Groll et al., 2009). …”

Mycobacterium kyorinenseInfection

“…This residue, Ser531 in the M. tuberculosis RpoB protein, was replaced by an Asp in M. kyorinense . Notably, Ser531 is the most frequent location of substitutions in rifampin-resistant strains of M. tuberculosis ( 6 ). …”