Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil

Ribeiro, Geovani de Oliveira; Monteiro, Fred Júlio Costa; Rêgo, Márlisson Octávio da Silva; Ribeiro, Edcelha Soares D’Athaide; Castro, Daniela Funayama de; Caseiro, Marcos Montani; Marinho, Robson dos Santos Souza; Komninakis, Shirley Vasconcelos; Deng, Xutao; Delwart, Eric; Sabino, Éster Cerdeira; Costa, Antônio Charlys da; Leal, Élcio

doi:10.3390/v11020147

Cited by 8 publications

(7 citation statements)

References 26 publications

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“…In agreement with previous virome studies, most of the taxa derived from honey-baited FTA cards have been identified in various invertebrates [39] and associated to mosquitoes [40]. Additionally, mosquito-specific viruses detected in FTA cards (Table 2) have been described as part of the viral communities harbored by several mosquito species in different geographic regions [41][42][43][44][45][46][47][48]. Bold type corresponds to the selected viruses for primers design.…”

Section: Virome Composition On Honey-baited Fta Cards During Entomolosupporting

confidence: 86%

Viromics on Honey-Baited FTA Cards as a New Tool for the Detection of Circulating Viruses in Mosquitoes

Birnberg

Temmam

Aranda

et al. 2020

Viruses

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Worldwide, emerging and re-emerging infectious diseases (EIDs) are a major burden on public and animal health. Arthropod vectors, with mosquitoes being the main contributors of global disease, transmit more than 70% of the recognized EIDs. To assess new alternatives for arthropod-borne viral diseases surveillance, and for the detection of new viruses, honey-baited Flinders Technology Associates (FTA) cards were used as sugar bait in mosquito traps during entomological surveys at the Llobregat River Delta (Catalonia, Spain). Next generation sequencing (NGS) metagenomics analysis was applied on honey-baited FTA cards, which had been exposed to field-captured mosquitoes to characterize their associated virome. Arthropod-and plant-infecting viruses governed the virome profile on FTA cards. Twelve near-complete viral genomes were successfully obtained, suggesting good quality preservation of viral RNAs. Mosquito pools linked to the FTA cards were screened for the detection of mosquito-associated viruses by specific RT-PCRs to confirm the presence of these viruses. The circulation of viruses related to Alphamesonivirus, Quaranjavirus and unclassified Bunyavirales was detected in mosquitoes, and phylogenetic analyses revealed their similarities to viruses previously reported in other continents. To the best our knowledge, our findings constitute the first distribution record of these viruses in European mosquitoes and the first hint of insect-specific viruses in mosquitoes' saliva in field conditions, demonstrating the feasibility of this approach to monitor the transmissible fraction of the mosquitoes' virome. In conclusion, this pilot viromics study on honey-baited FTA cards was shown to be a valid approach for the detection of viruses circulating in mosquitoes, thereby setting up an alternative tool for arbovirus surveillance and control programs.

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Section: Virome Composition On Honey-baited Fta Cards During Entomolosupporting

confidence: 86%

Viromics on Honey-Baited FTA Cards as a New Tool for the Detection of Circulating Viruses in Mosquitoes

Birnberg

Temmam

Aranda

et al. 2020

Viruses

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“…For example, the Amazon region is identified as the starting point for transmission of the yellow fever virus in a recent outbreak in Brazil that killed almost 700 people between December 2016 and March 2018 and the Amazon rainforest functions as a 'reservoir' region for several arboviruses [42]. Other studies have been identified novel virus presents in the mosquito microbiota in this region, in the host Aedes aegypti and anophelines [17,18,43], reinforcing the idea that our current knowledge about the diversity of viruses is still very limited. Furthermore, insect-specific virus (ISV) compose the majority of mosquito virome the virus-virus interactions may affect the transmission of some viral pathogens [44,45].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies from our group have described unusual viral sequences from Flaviridae and Reoviridae families in Ae. aegypti mosquitoes in the Amazon [17,18]. Using an NGS metagenomic approach, this study aimed to identify novel viruses in Ae.…”

Section: Introductionmentioning

confidence: 99%

Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species

Ribeiro

Morais

Monteiro

et al. 2020

Viruses

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Viruses are the most diverse and abundant microorganisms on earth, highly adaptive to a wide range of hosts. Viral diversity within invertebrate hosts has gained notoriety in recent years in public health as several such viruses have been of medical importance. Aedes aegypti serves as a vector for several viruses that have caused epidemics within the last year throughout Brazil; including Dengue, Zika and Chikungunya. This study aimed to identify new viral agents within Aedes aegypti mosquito in a city of the Amazonian region, where it is highly endemic. Metagenomic investigation was performed on 60 mosquito pools and viral RNA sequences present in their microbiota were characterized using genomic and phylogenetic tools. In total, we identified five putative novel virus species related to the Sobemovirus genus, Iflavirus genus and Permutatetraviridae family. These findings indicate a diverse taxonomy of viruses present in the mosquito microbiota of the Amazon, the region with the greatest invertebrate diversity in the world.

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“…Laboratory workflows can substantially affect the ability of metatranscriptomic sequencing to detect arboviruses in a mosquito pool. A popular way to increase sensitivity is by enriching for arbovirus using size filtration 16 , PEG precipitation 17 or sequence-independent amplification 12 . While this does increase the number of viral sequences, enrichment can also introduce bias 18,19 .…”

Section: Sensitivity and Specificity Of Metatranscriptomics As An Arbmentioning

confidence: 99%

Sensitivity and specificity of metatranscriptomics as an arbovirus surveillance tool

Batovska

Mee

Lynch

et al. 2019

Sci Rep

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be established for routine use of metatranscriptomic sequencing. The criterion for positive detection of a virus established in this paper is one example of a process that can be applied to produce comparable results, which also accounts for potential contamination found in the negative control. Further work utilising wild caught mosquitoes from diverse populations will help to establish metatranscriptomic sequencing as a tool that can broaden the capabilities of arbovirus surveillance.

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Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil

Cited by 8 publications

References 26 publications

Viromics on Honey-Baited FTA Cards as a New Tool for the Detection of Circulating Viruses in Mosquitoes

Viromics on Honey-Baited FTA Cards as a New Tool for the Detection of Circulating Viruses in Mosquitoes

Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species

Sensitivity and specificity of metatranscriptomics as an arbovirus surveillance tool

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