Detection of rs665862918 (15-bp Indel) of the HIAT1 Gene and its Strong Genetic Effects on Growth Traits in Goats

Gao, Jiayi; Song, Xiaoling; Wu, Hui; Tang, Qi; Wei, Zhiyan; Wang, Xinyu; Lan, Xianyong; Zhang, Bao

doi:10.3390/ani10020358

Cited by 8 publications

(6 citation statements)

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“…Body morphology traits reflect the growth and development of different parts of the body and are also important economic and breeding indicators for both meat and meat-wool sheep breeds ( 28 , 29 ). Existing studies have showed that the HIAT1 gene influenced both growth and meat production traits in goats, and that a 15-bp insertion in HIAT1 was associated with body morphology in Shaanbei white cashmere goats ( 19 , 30 ). In this study, a 9-bp insertion mutation located in the 5'-upstream region of the ovine HIAT1 gene (rs1089950828 -/GTCCAGTGG) was elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…HIAT1 is expressed in brown adipose tissue and is negatively correlated with the process in which glucose is converted to fat ( 18 ). Additionally, polymorphisms in HIAT1 have been shown to affect milk traits in buffalo as well as growth in goats ( 15 , 19 ). However, to date, there are few studies that have reported on the function of HIAT1 in indigenous Chinese sheep populations.…”

Section: Introductionmentioning

confidence: 99%

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The ovine HIAT1 gene: mRNA expression, InDel mutations, and growth trait associations

et al. 2023

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BackgroundThe hippocampal abundant transcript 1 (HIAT1) gene, also known as major facilitator superfamily domain-containing 14A (MFSD14A), encodes for a transmembrane transporter protein and has been previously shown to be associated with milk production in buffalo and sheep breeds, as well as growth traits in chicken and goats. However, tissue level distribution of the ovine HIAT1 gene, as well as its effect on body morphometric traits in sheep, has yet to be studied.MethodsThe HIAT1 mRNA expression profile of Lanzhou fat-tailed (LFT) sheep was determined by quantitative real-time PCR (qPCR). A total of 1498 sheep of three indigenous Chinese sheep breeds were PCR-genotyped for polymorphisms of HIAT1 gene. Student's t-test was used to observe the association between the genotype and sheep morphometric traits.ResultsHIAT1 was widely expressed in all examined tissues, and was particularly abundant in the testis of male LFT sheep. Additionally, a 9-bp insertion mutation (rs1089950828) located within the 5'-upstream region of HIAT1 was investigated in Luxi black-headed (LXBH) sheep and Guiqian semi-fine wool (GSFW) sheep. The wildtype allele frequency 'D' was found to be more prevalent than that of the mutant allele ‘I'. Furthermore, low genetic diversity was confirmed in all sampled sheep populations. Subsequent association analyses indicated an association between the 9-bp InDel mutation of interest and the morphometric traits of LXBH and GSFW sheep. Furthermore, yearling ewes with a heterozygous genotype (ID) demonstrated smaller body sizes, while yearling rams and adult ewes with the heterozygous genotype were found to have overall better growth performance.ConclusionThese findings imply that functional InDel polymorphism (rs1089950828) has the potential to be utilized for marker-assisted selection (MAS) of growth traits in domestic Chinese sheep populations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The ovine HIAT1 gene: mRNA expression, InDel mutations, and growth trait associations

et al. 2023

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“…Additionally, gene introns may contain cis-regulatory elements that participate in tissue-or stage-specific gene expression [28]. For instance, a novel intronic indel in the HIAT1 gene has strong genetic effects on growth traits in goats [43]. A previous study [18] used RNA hybrids (http: //bibiserv.techfak.uni-bielefeld.de/rnahybrid/ accessed on 19 January 2021) to predict miRNAs binding to the P2-13bp region.…”

Section: Discussionmentioning

confidence: 99%

Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (AKAP12) Gene and Their Effects on Growth Traits

Bai

Yuan

Luo

et al. 2021

Animals

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The A-kinase anchoring protein 12 gene (AKAP12) is a scaffold protein, which can target multiple signal transduction effectors, can promote mitosis and cytokinesis and plays an important role in the regulation of growth and development. In our previous study, P1–7 bp (intron 3) and P2–13 bp (3′UTR) indels within the AKAP12 gene significantly influenced AKAP12 gene expression. Therefore, this study aimed to identify the association between these two genetic variations and growth-related traits in Shaanbei white cashmere goats (SBWC) (n = 1405). Herein, we identified two non-linkage insertions/deletions (indels). Notably, we found that the P1–7 bp indel mutation was related to the height at hip cross (HHC; p < 0.05) and the P2–13 bp indel was associated with body weight, body length, chest depth, chest width, hip width, chest circumference and cannon (bone) circumference in SBWC goats (p < 0.05). Overall, the two indels’ mutations of AKAP12 affected growth traits in goats. Compared to the P1–7 bp indel, the P2–13 bp indel is more suitable for the breeding of goat growth traits.

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“…Two indel mutations in the intronic region of the ovine PITX2 gene have been found to have significant effects on ovine growth traits (Zhao et al ., 2017). A 15-bp indel on the intron of HIAT1 is detected to be relevant to the growth traits of goats and could be used for MAS as a molecular marker in goat populations (Gao et al ., 2020).…”

Section: Discussionmentioning

confidence: 99%

A novel 28-bp indel inIGF1Rgene associated with growth traits across four Chinese cattle breeds

Tang

Ma²,

Yang

et al. 2021

J. Agric. Sci.

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Insulin-like growth factor 1 receptor (IGF1R) is a cell surface receptor, belonging to the tyrosine kinase receptor superfamily. IGF1R plays a role not only in normal cell development but also in malignant transformation, which has become a candidate therapeutic target for the treatment of human cancer. This study aimed to explore insertions and deletions (indels) in IGF1R gene and investigate their association with growth traits in four Chinese cattle breeds (Xianan cattle, Jinnan cattle, Qinchuan cattle and Nanyang cattle). The current paper identified a 28-bp indel by polymerase chain reaction within IGF1R gene. The analysis showed that there was a significant correlation between the locus and the hucklebone width of Nanyang cattle in four periods, in which it was highly correlated at 6, 12 and 18 months. At the age of 6 months, it was also significantly correlated with body height, body weight and body length. Association analysis showed that the locus in Jinnan cattle was extremely significantly correlated with body slanting length and body weight, and significantly correlated with chest circumference. There was no significant correlation between this locus and growth traits of Xianan cattle and Qinchuan cattle. The detected indel in the IGF1R gene was significantly associated with growth traits in Jinnan and Nanyang cattle, and could be used as a molecular marker for growth trait selection.

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Detection of rs665862918 (15-bp Indel) of the HIAT1 Gene and its Strong Genetic Effects on Growth Traits in Goats

Cited by 8 publications

References 40 publications

The ovine HIAT1 gene: mRNA expression, InDel mutations, and growth trait associations

The ovine HIAT1 gene: mRNA expression, InDel mutations, and growth trait associations

Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (AKAP12) Gene and Their Effects on Growth Traits

A novel 28-bp indel inIGF1Rgene associated with growth traits across four Chinese cattle breeds

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