Detection of Salamonella Gallinarum and S. Typhimurium DNA in Experimentally Infected Chicks by Polymerase Chain Reaction.

Tuchili, L. M.; Kodama, Hiroshi; Izumoto, Yoko; Mukamoto, Masafumi; Fukata, Tsuneo; Baba, Tomoko

doi:10.1292/jvms.57.59

Cited by 14 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several PCR assays for detection of Salmonella have been developed, and several different target DNAs for amplification have been applied. Polymerase chain reaction assays which enable the detection of Salmonella in different sources, such as human or animal faeces (Widjojoatmodjo et al 1992;Mahon and Lax 1993;Cohen et al 1994aCohen et al , 1994bCohen et al , 1995Kongmuang et al 1994;Stone et al 1994;Haedicke et al 1996), various food samples (Cano et al 1993;Fluit et al 1993;Chen et al 1997), fish meat (Iida et al 1993;Lin and Tsen 1996), various meat products (Soumet et al 1994(Soumet et al , 1997Aabo et al 1995;Jitrapakdee et al 1995;Kwang et al 1996;Lin and Tsen 1996), chicken skin or organs (Mahon et al 1994;Tuchili et al 1995), eggs (Burkhalter et al 1995;Bäumler et al 1997), dairy products (Chevrier et al 1995;Cohen et al 1996), oysters (Jones et al 1993Bej et al 1994;Brasher et al 1998), feed (Cohen et al 1996), soil (Way et al 1993) and environmental water samples (Bej et al 1990b(Bej et al , 1991bWay et al 1993) have been described. The few assays described for the detection of Salmonella in water have been applied to water samples with low turbidity, such as autoclaved tap water and well water.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay

Waage¹,

Vardund²,

Lund³

et al. 1999

J Appl Microbiol

View full text Add to dashboard Cite

A polymerase chain reaction (PCR) assay with two nested pairs of primers selected from conserved sequences within a 2·3 kb randomly cloned DNA fragment from the Salmonella typhimurium chromosome was developed. The nested PCR assay correctly identified 128 of a total of 129 Salmonella strains belonging to subspecies I, II, IIIb and IV. One strain of Salm. arizona (ssp. IIIa) tested negative. No PCR products were obtained from any of the 31 non‐Salmonella strains examined. The sensitivity of the assay was 2 cfu, as determined by analysis of proteinase K‐treated boiled lysates of Salm. typhimurium. The performance of the assay was evaluated for environmental water, sewage and food samples spiked with Salm. typhimurium. Water and sewage samples were filtered and filters were enriched overnight in a non‐selective medium. Prior to PCR, the broth cultures were subjected to a rapid and simple preparation procedure consisting of centrifugation, proteinase K treatment and boiling. This assay enabled detection of 10 cfu 100 ml−1 water with background levels of up to 8700 heterotrophic organisms ml−1 and 10 000 cfu of coliform organisms 100 ml−1 water. Spiked food samples were analysed with and without overnight enrichment in a non‐selective medium using the same assay as above. Nested PCR performed on enriched broths enabled detection of < 10 cfu g−1 food. Variable results were obtained for food samples examined without prior enrichment and most results were negative. This rapid and simple assay provides a sensitive and specific means of screening drinking water or environmental water samples, as well as food samples, for the presence of Salmonella spp.

show abstract

Section: Introductionmentioning

confidence: 99%

“…1996; Lin & Tsen 1996), chicken skin or organs (Mahon et al . 1994; Tuchili et al . 1995), eggs (Burkhalter et al .…”

mentioning

confidence: 99%

Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay

Waage¹,

Vardund²,

Lund³

et al. 1999

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Salmonella detection by bacteriological methods usually requires 5 to 11 days (10, 27), and samples with low numbers of Salmonella cells, usually seen in subclinically infected chickens, may give false-negative results (7). Efforts have been made to reduce the time required and to increase the sensitivity of methods to detect Salmonella serovars in poultry samples (16,25). PCR with preincubation in an enrichment broth has been performed for human (5, 13, 14, 28), animal (6, 23, 24), fecal, and food (1,2,4,9,11,19) samples.…”

mentioning

confidence: 99%

Detection of Salmonellae in Chicken Feces by a Combination of Tetrathionate Broth Enrichment, Capillary PCR, and Capillary Gel Electrophoresis

Çarlı¹,

Unal²,

Caner³

et al. 2001

J Clin Microbiol

View full text Add to dashboard Cite

This report describes a rapid detection procedure for salmonellae from chicken feces by the combination of tetrathionate primary enrichment (preenrichment [PE])-bacterial lysis-capillary PCR and capillary gel electrophoresis. Pure Salmonella enterica serovar Enteritidis 64K was reisolated and detected by capillary PCR after buffered peptone water and nutrient broth, tetrathionate broth base Hajna (TTBH), and tetrathionate broth (TTB) preenrichments. When the same culture was mixed with intestinal homogenate, bacteriological reisolation and capillary PCR detection was achieved only by TTBH and TTB preenrichments. Capillary gel electrophoresis revealed that a Salmonella genus-specific 281-bp PCR product was detected when Salmonella strains but not non-Salmonella strains were tested. The detection limit of capillary PCR with whole-cell DNA extracted from pure Salmonella enterica serovars Enteritidis 64K, Typhimurium LT2-CIP60-62, and Gallinarum 64K was 3, 3, and 9 CFU ml ؊1 , respectively. The detection limit of capillary PCR from whole-cell DNA extracted from intestinal homogenate artificially contaminated with the same three strains was 3, 3, and 7 CFU ml ؊1 , respectively. We compared the results of the capillary PCR and bacteriological examination from the natural samples. Thirty-five of 53 naturally contaminated samples produced a specific PCR product. In 9 of the 35 PCR-positive samples, Salmonella could not be detected bacteriologically either by PE or a primary and delayed secondary enrichment (DSE) combination. In the 18 PCR-negative samples, 4 samples were found to harbor Salmonella by both PE and DSE and 14 samples were positive after DSE. Fifty-three additional intestinal homogenate samples, which were negative by their PE and DSE in bacteriological examination, were found to be also negative by their PCRs. The total time required to detect Salmonella with the capillary PCR method we used was approximately 20 h. If samples are from clinically diseased birds, the total time for PCR and detection is reduced to 2 h since the 18-h PE is not required. These results indicate that TTB enrichment, bacterial lysis, and genus-specific capillary PCR combined with capillary gel electrophoresis constitute a sensitive and selective procedure which has the potential to rapidly identify Salmonella-infected flocks.Salmonellae are among the major bacterial pathogens of poultry in Turkey and in the world (3,8). Prevention of Salmonella infection is important for poultry health and for the food industry, and prevention can be achieved only by good monitoring and screening programs (10,17,27). Salmonella detection by bacteriological methods usually requires 5 to 11 days (10, 27), and samples with low numbers of Salmonella cells, usually seen in subclinically infected chickens, may give false-negative results (7). Efforts have been made to reduce the time required and to increase the sensitivity of methods to detect Salmonella serovars in poultry samples (16,25). PCR with preincubation in an enrichment broth has been perform...

show abstract

“…Soumet et al (1997);Li et al (2000); Scholz et al (2001) andMyint et al (2006) proved that the high sensitivity and specificity of PCR needs about 16-24 hr. Many authors as Tuchili et al (1995) and Drawin and Miller (1999) selected the invA and explained that this gene was necessary for the invasion to the cell. Although, Lampel et al (2000);Ferretti et al (2001);Liu et al (2002) and Salehi et al (2005) supported the use of invA primer due to its accuracy and uniform distribution.…”

Section: Discussionmentioning

confidence: 99%

Surveillance for Salmonella in migratory, feral and zoo birds

Shalaby

Erfan

Nasef

2014

Suez Canal Veterinary Medical Journal. SCVMJ

View full text Add to dashboard Cite

In a surveillance targeting different birds communities, 237 cloacal swabs and intestinal samples from 39 different species of wild birds from different sources were examined bacteriologically for Salmonella. 32 samples were positive for Salmonella spp., with an incidence of 13.5%. Migratory birds had the highest incidence with a 28.26 percentage. The incidence in free living birds was somewhat lower (18.57%). While the Zoo birds had the lowest incidence (4.96%). Serotyping of the isolates revealed 6 different serotypes [Salmonella Typhimurium, Salmonella Rissen, Salmonella Regent, Salmonella Doncaster, Salmonella Curacao, Salmonella IIIb group (O65) and untyped Salmonella]. Salmonella Typhimurium represented 40.63% of the isolated serotypes. In vitro antibiogram test was performed for the 2 strains isolated from Zoo birds. Salmonella Curacao was sensitive to most of the utilized antimicrobial agents. However Salmonella IIIb group (O65) had surprising results as it was resistant to 10 out of the 11 applied antibiotics. 26 serum samples were examined by tube agglutination test using S. Typhimurium antigen where 15 samples were positive. PCR technique was done on 30 samples to assess the power of two different isolation enrichments[Rappaport vassiliadis (RV) and buffered peptone water (BPW)] in comparison to that of the standard microbioligical techniques (SMT). The detection percentages of RV-PCR, BPW-PCR and SMT was 66.7%, 46.7%, and 13.3%, respectively.

show abstract

Detection of Salamonella Gallinarum and S. Typhimurium DNA in Experimentally Infected Chicks by Polymerase Chain Reaction.

Cited by 14 publications

References 15 publications

Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay

Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay

Detection of Salmonellae in Chicken Feces by a Combination of Tetrathionate Broth Enrichment, Capillary PCR, and Capillary Gel Electrophoresis

Surveillance for Salmonella in migratory, feral and zoo birds

Contact Info

Product

Resources

About