Detection of Salmonella in Food Samples by Culture and Polymerase Chain Reaction Methods

Abstract

Conventional culture methods for the isolation and identification of food borne bacterial pathogens are rather sensitive and quite inexpensive, but at the same time they are labor-intensive and time-consuming. Molecular techniques are more rapid and highly sensitive for identification of food pathogens. This study was carried out to evaluate a 12 hour PCR method for detection of Salmonella in food samples. The results showed that out of 150 food samples, 32 (21.3%) were positive by culture, 35 (23.3%) were positive by PCR, the sensitivity of PCR was 100% while specificity was 97.5%. The study concluded that the 6-h enrichment followed by PCR was rapid, simpler method that allowed the detection of Salmonella spp. within a maximum of 12 h.

Elucidation of rRNA Secondary Structure and Phylogenetic Analysis of Salmonella enterica based on 16S RNA

“…The conventional methods for the detection of Salmonella require multiple steps which may take up to 5 to 7 days (Ahmed et al, 2014). The final identification and characterization of Salmonella enterica is based on biochemical reactions followed by serotyping.…”

Salmonellosis: Food-borne Plague

“…The microbiological culture techniques for the confirmatory detection of Salmonella are generally performed but these techniques stick with low sensitivity, intensive labor requirements, time-consuming, not cost-effective, lack trained professionals and the results require weeks to confirm presence and absence of Salmonella. The time-efficient methods of diagnosis have prime importance as it is necessary for the treatment of clinical complications and to confirm the origin of that pathogen (Odumeru and León-Velarde 2012;Zadernowska and Chajecka 2012;Almeida et al 2013;Ahmed et al 2014).…”