Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR

Abstract

New Salmonella Typhi conjugate vaccines have been deployed, which will potentially lead to a fall in incidence rates of typhoid fever in endemic areas. Identification of chronic carriers of S. Typhi will be important as these individuals can be a potential source of transmission to susceptible persons.

Incidence of typhoid fever in Burkina Faso, Democratic Republic of the Congo, Ethiopia, Ghana, Madagascar, and Nigeria (the Severe Typhoid in Africa programme): a population-based study

“…Confirmatory qPCR was performed using the STY0201 and oriC probe sets as previously described. 16 , 17 For whole genome sequencing, genomic DNA was extracted from isolates using the Wizard Plus Genomic Purification kit (Promega, Madison, WI, USA) and sequenced by Eurofins Genomics (Ebersberg, Germany). Raw reads were assembled and the serovar was determined using the Global Health Research Unit pipeline.…”