2020
DOI: 10.18821/0869-2084-2020-65-12-785-792
|View full text |Cite
|
Sign up to set email alerts
|

Detection of SARS-CoV-2 RNA in nasopharyngeal swabs from COVID-19 patients and asymptomatic cases of infection by real-time and digital PCR

Abstract: In this work we tested two reagent kits developed by us for detecting SARS-CoV-2 RNA using a fragment of the ORF1ab gene in digital PCR and real-time PCR formats. Data were obtained on the detection of SARS-CoV-2 virus RNA in nasopharyngeal swabs of patients with COVID-19 and asymptomatic carriers. The developed reagent kits provided 100% sensitivity and a detection limit of 103 GE / ml for qPCR, and at least 200 copies / ml of viral RNA when performing digital PCR. These methods were tested using a panel of 1… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
5
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 9 publications
(5 citation statements)
references
References 21 publications
0
5
0
Order By: Relevance
“…Fifth, for resource, microbiological and logistical reasons, we did not perform nasopharyngeal swabs for the entire cohort and therefore could not exclude the possibility that some participants could harbor asymptomatic infection on recruitment. However, diagnosing asymptomatic infection can be challenging as the sensitivity of quantitative PCR could be as low as 30% in asymptomatic individuals depending on whether the test is performed during the incubation, presymptomatic, or recovery phases of the disease (Dong et al, 2021;Ternovoi et al, 2020). To further improve its accuracy, repeated PCR tests may be required.…”
Section: Discussionmentioning
confidence: 99%
“…Fifth, for resource, microbiological and logistical reasons, we did not perform nasopharyngeal swabs for the entire cohort and therefore could not exclude the possibility that some participants could harbor asymptomatic infection on recruitment. However, diagnosing asymptomatic infection can be challenging as the sensitivity of quantitative PCR could be as low as 30% in asymptomatic individuals depending on whether the test is performed during the incubation, presymptomatic, or recovery phases of the disease (Dong et al, 2021;Ternovoi et al, 2020). To further improve its accuracy, repeated PCR tests may be required.…”
Section: Discussionmentioning
confidence: 99%
“…ddPCR outperforms other molecular approaches in terms of repeatability, stability, sensitivity, and specificity [ 49 ]. It enables physicians to diagnose COVID-19 infection in individuals who are asymptomatic or paucisymptomatic by accurately detecting extremely low viral loads [ 50 ]. According to a recent study, 63 samples that had previously tested negative for SARS-CoV-2 by RT-qPCR were positive for the virus using ddPCR, and 55% of those samples displayed COVID-19 symptoms [ 51 ].…”
Section: Nucleic Acid Amplification Techniques (Naats)mentioning
confidence: 99%
“…However, most of the reported ddPCR procedures included an RNA extraction and purification step, which can lead to potential amplification errors [ 38 ]. Moreover, direct quantification by ddPCR targeting the envelope (E) gene [ 39 ], ORF1ab gene [ 40 ], and nucleocapsid (N) [ 41 ] region have also been reported. The viral load can be quantified in throat swabs, sputum, nasal swabs, blood, and urine [ 37 ].…”
Section: Rt-pcr Biosensorsmentioning
confidence: 99%