Detection of Sclerotinia sclerotiorum in sunflower by enzyme-linked immunosorbent assay (ELISA)

Walcz, Ilona; Pacsa, A.S.; Emödy, Levente; Szabó, Lajos

doi:10.1016/s0007-1536(85)80044-9

Cited by 11 publications

(4 citation statements)

References 9 publications

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“…There are numerous indirect methods available for detecting these plant diseases including uorescence in situ hybridization, 1 immunouorescence, 2,3 ow cytometry, 4 gas chromatography, mass spectroscopy, [5][6][7][8] and others. [9][10][11] However all these methods are time consuming, demand skilled analysts and do not offer online, real time early detection possibilities. Early detection of plant disease infections is critical to minimize agricultural losses and increase crop productivity.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive electrochemical detection of methyl salicylate using electroactive gold nanoparticles

Umasankar

Ramasamy

2013

Analyst

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Electrochemical sensing of methyl salicylate, a key plant volatile has been achieved using a gold nanoparticle (AuNP) modified screen printed carbon electrode (SPCE). The electrochemical response of planar gold electrodes, SPCE and AuNP-SPCE in alkaline electrolyte in the presence and absence of methyl salicylate were studied to understand the amperometric response of various electrochemical reactions. The reaction mechanism includes hydrolysis of methyl salicylate and the oxidation of negative species. The electrochemical responses were recorded using cyclic voltammetry and differential pulse voltammetry techniques, where the results showed characteristic signals for methyl salicylate oxidation. Among the examined electrodes, AuNP-SPCE possessed three fold better sensitivity than planar gold and 35 times better sensitivity than SPCE (at 0.5 V). The methyl salicylate sensing by AuNP-SPCE possessed <5% variation coefficient for repeatability, one week of stable performance with no more than 15% activity loss even if used multiple times (n = 8). Even in the presence of high concentration of interfering compounds such as cis-3-hexenol, hexyl acetate and cis-hexenyl acetate, AuNP-SPCE retained >95% of its methyl salicylate response. The electroanalytical results of soybean extract showed that AuNP-SPCE can be employed for the determination of methyl salicylate in real samples.

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Section: Introductionmentioning

confidence: 99%

Highly sensitive electrochemical detection of methyl salicylate using electroactive gold nanoparticles

Umasankar

Ramasamy

2013

Analyst

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“…2 Both direct as well as indirect methods are available for disease detection in plants. For example, molecular methods such as polymerase chain reaction and fluorescence in situ hybridization, and serological methods such as flow cytometry, 3,4 enzyme-linked immunosorbent assay [5][6][7] and immunofluorescence, 8,9 can be used as direct detection methods for identification of plant diseases. 10 Disease detection can also be achieved by sampling the plant volatile signature.…”

Section: Introductionmentioning

confidence: 99%

Electroanalytical studies on green leaf volatiles for potential sensor development

Umasankar

Rains

Ramasamy

2012

Analyst

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An electrochemical study for detecting green leaf plant volatiles from healthy and infected plants has been devised and tested. The electrocatalytic response of plant volatiles at a gold electrode was measured using cyclic voltammetry, amperometric current-time (i-t) analysis, differential pulse voltammetry (DPV) and hydrodynamic experiments. The sensitivity of the gold electrode in i-t analysis was 0.13 mA mM(-1) cm(-2) for cis-3-hexenol, 0.11 mA mM(-1) cm(-2) for cis-hexenyl acetate and 0.02 mA mM(-1) cm(-2) for hexyl acetate. The limits of detection of cis-3-hexenol, cis-hexenyl acetate and hexyl acetate by i-t analysis were 0.5, 0.3 and 0.6 μM, respectively, at a signal to noise ratio of 3. The hydrodynamic studies yielded the electro-kinetic parameters such as diffusivities of plant volatiles in solution and the rate constants for their electrochemical reactions. The DPV and interference studies reveal that the gold electrode possessed high sensitivity for plant volatiles determination in synthetic samples, which imitates both healthy and infected plants.

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“…An alternative approach to early detection of the ftmgtis would be the use of serological procedures such as enzyme-linked immunosorbent assay (ELISA). Detection of numerous ftmgal pathogens in host tissues by ELISA has already been reported but there have been difficulties in obtaining highly specific antisera (Walcz et at.. 1985;Amouzou-Alladaye et ai, 1988;Barker & Pitt, 1988;Mohan, 1988;Fegics, 1991;Ricker er a/., 1991;Lyons & White, 1992). This is because fungi are very complex organisms that contain ntimerous antigens, many of which are shared by unrelated fungi.…”

Section: Introductionmentioning

confidence: 99%

Development of a polyclonal antibody‐based immunoassay for the early detection of Sclerotinia sclerotiorum in rapeseed petals

Jamaux

Spire

1994

Plant Pathology

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A serological test has been developed that allows the early detection of itifection of young petals by Sclerotinia sclerotiorum, an important pathogen of rapeseed. Two steps were required to obtain an antisertim sufficiently specific for 5. sclerotiorum. Soluble mycelial extracts of 5. sclerotiorum were used to produce the first generation polyclonal antiserum. This was not specific for 5. sclerotiorum in double antibody sandwich etizyme-Utiked immunosorbent assay (DAS-ELISA) and allowed the screening of cross-reacting ftmgal species such as Botrytis cinerea. a pathogen commonly present on rapeseed petals. Using a polyclonal anti-.B. cinerea senim enabled the absorption, by serial cycles, of S. sclerotiorum antigens common to B. cinerea. Residtial antigens were then used as immunogens for the production of two second generation antisera (SI and S2) which were then tested by DAS-ELISA. Cross-reactions with B. cinerea decreased with purification cycles of the immunogen whereas cross-reactions with some unrelated fungi slightly increased. S. sclerotiorum and B. cinerea were distinguishable using antiserum S2.

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Detection of Sclerotinia sclerotiorum in sunflower by enzyme-linked immunosorbent assay (ELISA)

Cited by 11 publications

References 9 publications

Highly sensitive electrochemical detection of methyl salicylate using electroactive gold nanoparticles

Highly sensitive electrochemical detection of methyl salicylate using electroactive gold nanoparticles

Electroanalytical studies on green leaf volatiles for potential sensor development

Development of a polyclonal antibody‐based immunoassay for the early detection of Sclerotinia sclerotiorum in rapeseed petals

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