Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma

Zhao, Wei; Li, Juan; Zhang, Junjie; Gao, Pengfei; Pei, Hang; Wang, Lei; Guo, Fei; Yu, Jian; Zheng, Shu; Wang, Jiaxiang

doi:10.3390/ijms160612669

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…Zhao W proposed that a low level of AFP at the first visit is an indicator of poor prognosis. 15 The results of the present study show that the prognoses were relatively good in those with infantile HB who have a relatively high level of AFP. However, the prognoses of pediatric patients with AFP<100ng/mL were poor, which was consistent with relevant foreign reports.…”

Section: Discussionsupporting

confidence: 48%

Clinical Characteristics and Prognosis Analysis of Infantile Hepatoblastoma—A 15-Year Retrospective Single-Center Study

Zhi

Zhang

et al. 2021

CMAR

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Objective The present study aimed to summarize the clinical data of hepatoblastoma (HB) in infants under one year of age and to analyze the factors that affected the prognoses. Methods The clinical data of 132 pediatric patients with a pathologically confirmed HB, aged less than one year and who had visited the Pediatric Single Center of Beijing Tongren Hospital from May 2005 to May 2019, were retrospectively analyzed to summarize the clinical outcomes and prognoses. Results The male/female ratio was 1.27 and the median age was 8.40 months. The onset of HB was usually characterized by abdominal bulging (75.0%). The median level of AFP at the first visit was 154.7µg/mL, and the average platelet count was (405±166)×10 9 /L. The epithelial type (57.6%) was the predominant pathological type, and stage III (54.5%) was the main PRETEXT staging. Distant metastases occurred in 45 cases, with pulmonary metastases (86.7%) being the most common site. At the time of visit, 24 cases (18.2%) had either portal vein, hepatic vein, or vena cava infiltration. Five cases (3.8%) had a hemorrhage of the ruptured tumor, and 26 cases (19.7%) had multiple intrahepatic foci. At the follow-up in May 2020, the overall survival (OS) rate at one, three, and five years of age was 94.3%, 88.8%, and 80.1%, respectively, and the event-free survival rate was 91.8%, 86.9%, and 77.5%, respectively, by the Kaplan–Meier survival analysis. According to the Log rank test, pediatric patients with an AFP <100ng/mL, a PRETEXT stage IV, presence of distant metastases and multiple foci of the primary tumor at the initial diagnosis had poorer prognoses (P<0.05). Conclusion The prognosis of HB in infancy is relatively good, but is still vulnerable to multiple factors, such as tumor features leading to different AFP levels, PRETEXT stage, presence of distant metastases, and multiple intrahepatic foci.

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Section: Discussionsupporting

confidence: 48%

Clinical Characteristics and Prognosis Analysis of Infantile Hepatoblastoma—A 15-Year Retrospective Single-Center Study

Zhi

Zhang

et al. 2021

CMAR

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“…Significantly high or low serum AFP was associated with adverse prognosis [8]. Serum protein markers were analyzed using mass spectrometry, and of the target proteins isolated, Apo A-I was found to have increased expression in the HB subgroups compared to normal [10]. In addition, DNA methylation patterns may be used as predictors of outcome.…”

Section: Biologymentioning

confidence: 99%

Hepatoblastoma—The Evolution of Biology, Surgery, and Transplantation

et al. 2018

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The most common primary malignant liver tumor of childhood, hepatoblastoma has increased in incidence over the last 30 years, but little is still known about its pathogenesis. Discoveries in molecular biology provide clues but have yet to define targeted therapies. Disease-free survival varies according to stage, but is greater than 90% in favorable risk populations, in part due to improvements in chemotherapeutic regimens, surgical resection, and earlier referral to liver transplant centers. This article aims to highlight the principles of disease that guide current treatment algorithms. Surgical treatment, especially orthotopic liver transplantation, will also be emphasized in the context of the current Children’s Oncology Group international study of pediatric liver cancer (AHEP-1531).

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“…Separation and purification of a protein from complex biological specimens is a complicated procedure. Solid phase extraction (SPE), high-performance liquid chromatography (HPLC) and tricine-SDS-PAGE are the main technologies used to separate and purify low-abundance proteins from serum, urine or tissue lysate 28 , 29 . After the identification of the biomarker, verification is used to verify the different expression of the proteins through immune methods or other quantitative approaches.…”

Section: Introductionmentioning

confidence: 99%

Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach

Zhai

et al. 2017

Sci Rep

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Colorectal cancer (CRC) is a common malignant neoplasm worldwide. It is important to identify new biomarkers for the early detection of CRC. In this study, magnetic beads and the Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) platform were used to analyse CRC and healthy control (HC) serum samples. The CRC diagnosis pattern was established to have a specificity of 94.7% and sensitivity of 92.3% in a blind test. The candidate biomarker serine/threonine kinase 4 (STK4, also known as MST1) was identified by Tandem mass spectrometry (MS/MS) and verified with western blotting and enzyme-linked immunosorbent assay (ELISA). The results indicated that there was a higher concentration of MST1 in HC subjects than stage I CRC patients for the early detection of CRC and a lower concentration in stage IV patients than in other CRC patients. The sensitivity and specificity of MST1 combined with carcinoembryonic antigen (CEA) and faecal occult blood test (FOBT) in diagnosis of colorectal cancer were 92.3% and 100%, respectively. Additionally, low MST1 expression was associated with the poor prognosis. These results illustrate that MST1 is a potential biomarker for early detection, prognosis and prediction of distant metastasis of CRC.

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Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma

Cited by 6 publications

References 30 publications

Clinical Characteristics and Prognosis Analysis of Infantile Hepatoblastoma—A 15-Year Retrospective Single-Center Study

Clinical Characteristics and Prognosis Analysis of Infantile Hepatoblastoma—A 15-Year Retrospective Single-Center Study

Hepatoblastoma—The Evolution of Biology, Surgery, and Transplantation

Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach

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