Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

Abstract

Cow mastitis caused by Escherichia coli (E. coli) exhibits various local and systemic clinical signs at varying degrees of severity. The aim this study was to elucidate the virulence properties, antibiotic resistance and phylogenetics of 56 E. coli strains. Of all the studied strains, 12 were positive for hemolytic properties and 38 were positive for biofi lm production. Additionally, 55 of the strains were positive for multiple resistances in bacteriological tests. PCR analysis revealed that 42 strains carried the traT gene, 20 strains had the shiga toxin gene (stx1-stx2), and 8 strains carried the intimin gene (eae), but all strains were negative for aerobactin gene (aer). All strains encoding shiga toxin genes were also positive for stx1, but only 4 strains were positive for stx2. There were no signifi cant differences in virulence genes between antibiotic-resistant and antibiotic-susceptible strains. The random amplifi ed polymorphic DNA-polymerase chain reaction patterns revealed the existence of 13 main groups with 4 subgroups of E. coli. In this study, E. coli strains causing intramammary infections and originating from various sources might show resistance against common antibiotics. Pathogenity of E. coli that cause clinical mastitis, and prognosis of the infection might be predicted by obtaining the traT gene. Additionally, antibiotic resistance should be investigated at the genomic level to detect the relationship between virulence factors and antibiotic resistance. In fi eld conditions, development of antibiotic resistance is the main cause of mastitis treatment failure. Thus, antibiotic resistance profi les in herds should be monitored and effective antibiotics should be administered.

Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah

“…Prevalensi E. coli hemolitik asal susu dilaporkan bervariasi, hasil penelitian yang lebih kecil dilaporkan 12,7% dan 5,6% (Lamey et al, 2013dan Sayed, 2014. Serta prevalensi yang lebih besar dilaporkan 21,4%; dam 52,94% (Seyda et al, 2014dan Younis et al, 2018. Prevalensi E. coli hemolitik dari susu sapi dan unta masing-masing adalah 25% dan 32% (Nato et al, 2019).…”

Survey and Sequence Characterization of Bovine Mastitis-Associated Escherichia coli in Dairy Herds

“…Moreover, tetracyclines and sulphonamides are preferred antibiotics for treatment of mastitis caused by gram-negative pathogens (62,63). Internationally, it has been shown in Turkish cow herds where mastitis is managed with the fluoroquinolones (danofloxacin and enrofloxacin), E. coli was shown to have notable resistance to these antibiotics (61). In China, mastitisassociated E. coli have been shown to have notable resistance to sulfamonomethoxine and sulfamethoxazole, both used in management of intestinal infections (19).…”

Mastitis on selected farms in Wakiso district, Uganda: Burden, pathogens and predictors of infectivity of antimicrobial resistant bacteria in dairy herds

“…Mastitis remains a frequent (Ruegg, 2017 ) and economically important disease of dairy cattle, characterized by udder inflammation with cases being accompanied by either physical, chemical or bacteriological changes in milk (Hamadani et al., 2013 ). It is the costliest disease of dairy cattle (Liang et al., 2017 ) causing reduced milk yield, increased health management costs and making milk less suitable for consumption and processing (Cengiz et al., 2014 ). In addition, it is viewed as the main cause of antibiotic use in adult dairy cows, a key driver of antimicrobial resistance (AMR).…”