Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA in the Human Eye

Kamalipour, Alireza; Ashraf, M.; Moghimi, Sasan; Moattari, Afagh; Abbasi, Farhad; Azodi, Farzan; Oboudi, Shadi; Pirbonyeh, Neda; Mokhtaryan, Maryam; Roshanshad, Amirhossein; Jiun, L.; Weinreb, Robert N.

doi:10.1080/09273948.2021.1980810

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…In addition, negative results have been explained by the view that pathological changes may not be permanent in individuals succumbing to COVID-19 [16]. In a larger study evaluating 28 conjunctiva, 30 aqueous humor, and 30 vitreous fluid, SARS-CoV-2 RNA was evaluated in postmortem ocular specimens, and positive results were found in 1 conjunctival and 2 vitreous specimens in 3 different patients [17]. Compared to the aforementioned studies, the higher number of samples in this study may have resulted in positive results.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the presence of SARS-CoV-2 in the aqueous humor and vitreous in patients undergoing combined phaco-vitrectomy surgery

et al. 2023

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Purpose: To investigate the presence of virus in the aqueous humor and vitreous of patients undergoing elective combined cataract and pars plana vitrectomy during the Coronavirus disease 2019 (COVID-19) pandemic. Materials and methods: In this prospective cross-sectional study, of the patients to undergo elective surgery, those who had a negative severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2) nasal swab test 24-72 hours prior to the surgery and who were asymptomatic were included. SARS-CoV-2 IgG antibodies were evaluated in blood samples taken 24-72 hours before the operation. 0.1 cc of aqueous humor and 0.1 cc of vitreous fluid were aspirated at the beginning of the surgery. The presence of SARS-CoV-2 viral ribonucleic acid (RNA) was evaluated by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Results: Of 66 participants, 39 were male (59.1%) while 27 were female (40.9%). Twenty five patients (37.8%) had a history of COVID-19 between 20 days - 60 days (mean 49 days) before the surgery. There were 58 patients (87.9%) with a history of vaccination before the operation and 8 patients (12.1%) without a history of vaccination. No SARS CoV 2 RNA was detected in the aqueous humor and vitreous samples of any patient. IgG against SARSCoV-2 was detected in 3 patients who had not been vaccinated against COVID-19 before and had no known history of COVID-19. Conclusion: We did not find any SARS-CoV-2 viral genetic material in the aqueous and vitreous fluids of asymptomatic participants whose nasal swab test was negative, even if they recently had COVID-19.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the presence of SARS-CoV-2 in the aqueous humor and vitreous in patients undergoing combined phaco-vitrectomy surgery

et al. 2023

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“…14,20,21 Theoretically, autoimmunity might result from the cGAS abnormally detecting self-DNA, according to the original definition of intracellular DNA sensing. [22][23][24] Numerous varieties of DNA damage, both exogenous and endogenous, are possible sources of these DNAs. In the case of mitochondrial failure, the mitochondrial DNA (mtDNA), which is a rich reservoir of extranuclear self-DNA, might leak out into the cytoplasm.…”

Section: The C G a S-s Ting-irfa Xismentioning

confidence: 99%

“…Despite viral infection, cGAS stimulation can also be triggered by host DNA 14,20,21 . Theoretically, autoimmunity might result from the cGAS abnormally detecting self‐DNA, according to the original definition of intracellular DNA sensing 22–24 . Numerous varieties of DNA damage, both exogenous and endogenous, are possible sources of these DNAs.…”

Section: The Cgas–sting–irf3 Axismentioning

confidence: 99%

Harnessing cGAS–STING axis for therapeutic benefits in systemic lupus erythematosus

Chang

2024

Int J of Rheum Dis

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The cyclic GMP–AMP synthase (cGAS), a prominent intracellular DNA sensor in mammalian cells, controls the innate immune response and the stimulator of interferon genes (STING)‐mediated synthesis of pro‐inflammatory cytokines, such as type‐I interferon (IFN‐I). For decades, IFN‐I has been hypothesized to be essential in the development of systemic lupus erythematosus (SLE), a chronic multisystem autoimmunity characterized by immune complex (IC) deposition in small vessels. Recent findings revealed that the activation of the cGAS–STING pathway by self‐DNA would propagate the autoimmune responses via upregulating IFN‐I production in SLE. In this review, we aimed to provide a comprehensive outlook of the role of the cGAS–STING pathway in SLE pathobiology, as well as, a better understanding of current therapeutic opportunities targeting this axis.

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Functional and therapeutic significant of heat-shock protein 90 (HSP90) in reproductive cancers

He,

Wang

2024

Clin Transl Oncol

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Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA in the Human Eye

Cited by 4 publications

References 48 publications

Evaluation of the presence of SARS-CoV-2 in the aqueous humor and vitreous in patients undergoing combined phaco-vitrectomy surgery

Evaluation of the presence of SARS-CoV-2 in the aqueous humor and vitreous in patients undergoing combined phaco-vitrectomy surgery

Harnessing cGAS–STING axis for therapeutic benefits in systemic lupus erythematosus

Functional and therapeutic significant of heat-shock protein 90 (HSP90) in reproductive cancers

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