Detection of Shiga toxigenic (STEC) and enteropathogenic (EPEC) Escherichia coli in dairy buffalo

Diarrheagenic Escherichia coli

“…In general, these studies have shown that despite serotype diversity, the stx subtypes and the virulence profile identified among isolates from the animal reservoir and environment are similar to the isolates recovered from patients 173, 178, 179, 180. There has been particular interest in some STEC serotypes that have been responsible for causing severe human infections, such as O113:H21, but unlike others, they do not produce adhesins encoded by LEE.…”

“…The role of different animal species as asymptomatic carriers of EHEC/STEC has been extensively studied in the last years in Brazil. Besides cattle, which are their most common natural reservoir,173, 178 the presence of these pathogens has been identified in the feces of dairy buffaloes, 179 sheep,180, 181 pigs,182, 183 birds, 184 and fishes 185 . It is noteworthy that some relevant serotypes linked to human infections such as O103:H2 and O157:H7 have been recovered from the feces of sheep 186 and cattle 173 respectively.…”

Shiga toxin‐producingEscherichia coliin the animal reservoir and food in Brazil

“…On the other hand, the stx1/stx2 genotype was the prevalent one in studies conducted in the same region by Cerqueira et al (, ) and Irino et al () with dairy cattle; Joaquim et al () with cattle (dairy or beef), Oliveira et al () with beef cattle and dairy goats and Vettorato et al (, ) and Maluta et al () with sheep. The stx2 genotype predominated in studies of Vicente et al () and Oliveira et al () with dairy cattle and Beraldo et al () with dairy buffalos.…”

“…A wide variety of serotypes were also described, with the predominance of O22:H8, O79:H14, O91:H21 and O116:H21 (Oliveira et al ). Another study involving dairy buffalo conducted in SP found the prevalence of STEC to be 28·9% (Beraldo et al ).…”

“…The majority of STEC strains isolated from cattle or food of cattle origin by Cerqueira et al (), Cerqueira et al () and Gonzalez et al () were enterohaemolytic and also eae ‐negative. STEC strains are frequently ehxA‐ positive, with frequencies varying from around 35% to as high as 90%, and it is generally similar among strains isolated from either diarrheic or healthy animals and from eae ‐positive or ‐negative STEC strains (Leomil et al ; Salvadori et al ; Vetoratto et al ; Joaquim et al ; Irino et al ; Aidar‐Ugrinovitch et al ; Farah et al ; Oliveira et al ; Pigatto et al ; Beraldo et al ; Maluta et al ; Martins et al ; Gonzalez et al ). However, Coura et al () detected this gene only among eae ‐positive strains isolated from calves, both diarrheic and healthy ones.…”