Detection of silver nanoparticles inside leaf of European beech (Fagus sylvatica L.)

Abstract

In a greenhouse experiment, silver nanoparticles (Ag-NPs) were applied on European beech (Fagus sylvatica L.) leaves using the droplet application method. Scanning electron microscopy (SEM) analyses showed that after 24 h silver nanoparticles were mostly present in aggregates or as single particles on the surface of the leaf, surrounding or covering the stomata. Analyses of cross sections of the leaf revealed that some silver nanoparticles were adhering to the cell walls of the mesophyll and palisade cells, most likely after penetration into the leaf through the stomata as particles and not as Ag ions. Our preliminary results showed evidence of foliar uptake of silver nanoparticles in European beech. This opens new insights on the ability of trees to take up solid nanosized particles, eventually contained in raindrops, through their leaves, and potentially transport them to other parts of the tree. This study would be helpful for investigating the role of trees in atmospheric ultrafine particle mitigation.

Eco-Friendly Silver Nanoparticles Synthesized from a Soybean By-Product with Nematicidal Efficacy against Pratylenchus brachyurus

“…This complexity can be attributed to various factors, including the absorption of AgNPs by plant roots and their attachment to soil particles, resulting in dynamic nanoparticle distributions in the vicinity of the roots [ 61 ] and their interactions with the multifaceted rhizosphere microbial community [ 62 ]. Such behavior, highlighted in recent studies like that of [ 63 ], points to the intricate challenge of predicting nanoparticle effects in natural settings and underscores the need for extensive research to unravel the underlying mechanisms at play.…”

Eco-Friendly Silver Nanoparticles Synthesized from Soybean By-Product with Nematicidal Efficacy against <em>Pratylenchus brachyurus</em>

“…This complexity can be attributed to various factors, including AgNPs absorption by plant roots and their attachment to soil particles, resulting in dynamic nanoparticle distributions in the vicinity of the roots [61], and their interactions with the multifaceted rhizosphere microbial community [62]. Such behavior, highlighted in recent studies like that of [63], points to the intricate challenge of predicting nanoparticle effects in natural settings and underscores the need for extensive research to unravel the underlying mechanisms at play.…”