Detection of single-stranded nucleic acids by hybridization of probe oligonucleotides on polystyrene nanospheres and subsequent release and recovery of fluorescence

Performance Analysis of Electrochemical Detection Platform for DNA Hybridization Using TGN-Based Nanobiosensor

“…[6][7][8][9][10][11][12] Deoxyribonucleic acid (DNA), a genetic material, has been used in different biological applications, such as disease prevention, inflammation inhibition, tissue regeneration, bioimaging, biosensing, diagnosis and therapeutics. [13][14][15][16][17][18][19][20] In DNA hybridization, the target, unknown single-stranded DNA (ssDNA), is recognized and created by a probe single-stranded DNA (ssDNA) and a double-stranded DNA (ds-DNA) helix structure with two complementary strands. It can be stated that, the hybridization reaction is known to be extremely efficient and specific in the presence of a mixture of diverse non-complementary nucleic acids.…”

“…Some ssDNAs with secondary structures by self-pairing which is named aptamer, have the potential of targeting, which are selected via systematic evolution of ligands with exponential enrichment. [13][14][15][16][17][18][19][20] DNA-based applications in nanobiotechnology have been investigated during recent years. [20][21][22][23][24][25][26][27][28] DNA-based nanobiosensors have paved new way for researchers to reconstruct metabolic pathways in cells, and give simple, fast, cost-effective and sensitive detection of diseases including immunodeficiency and neurological.…”