Detection of single tumor cell resistance with aptamer biochip

Wang, Lixue; Zheng, Qi; Zhang, Quan'an; Xu, Hui; Tong, Jiayi; Zhu, Chuandong; Wan, Yuan

doi:10.3892/ol.2012.890

Cited by 24 publications

(11 citation statements)

References 29 publications

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“…Con el algoritmo "Droplet routing" aplicado al dispositivo, se prueba entonces todo el biochip usando menor tiempo [19]. Con relación a nucleicos, se ha desarrollado, por último, un biochip conformado por un aptámero de ARN para la captura de células tumorales y la detección de resistencia a células individuales [20].…”

Section: Algoritmounclassified

Biochips, aplicaciones convencionales e innovación: Una revisión documental

Sarmiento

Vélez

2019

Investig. innov. ing

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Objetivo: Analizar las tendencias de la aplicación convencional de los biochips, para lo cual se realizó una revisión documental en torno a las tendencias en la creación de biochips a partir de una búsqueda y análisis de los trabajos publicados desde el año 2012 hasta el 2017 en la base de datos de la National Center for Biotechnology Information (NCBI). Resultados: Se encontró que las tendencias en la creación de biochips se han configurado como una innovación relativamente nueva en el campo de la Ingeniería Bioinformática aplicada en otras áreas, y en particular la medicina, para el tratamiento y diagnóstico de enfermedades como el cáncer. Asimismo, se encontró que desde la química y la genética se han desarrollado materiales para la fabricación de los biochips, haciéndolos biocompatibles para el organismo humano. Conclusiones: El desarrollo de nuevas tecnologías, usando biochips para la detección y monitoreo de enfermedades, permite la creación de tratamientos más efectivos y puntuales en pro de la salud y la calidad de vida. Así se evitan, además, complicaciones futuras de los pacientes, dado que su implementación sugiere una técnica menos traumática e invasiva.

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Section: Algoritmounclassified

Biochips, aplicaciones convencionales e innovación: Una revisión documental

Sarmiento

Vélez

2019

Investig. innov. ing

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“…Anti-EGFR aptamers have also been used in a unique way by Wang et al This group generated a biochip with RNA aptamers coating a microelectrode on a silicon dioxide layer [67]. The biochip contains a PDMS cover with a channel for introducing cells.…”

Section: Aptamer-based Technologies For Ctc Enrichment/capturementioning

confidence: 99%

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

Dickey

Giangrande

2016

Methods

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A critical challenge for treating cancer is the early identification of those patients who are at greatest risk of developing metastatic disease. The number of circulating tumor cells (CTCs) in cancer patients has recently been shown to be a valuable (and non-invasively accessible) diagnostic indicator of the state of metastatic disease. CTCs are rare cancer cells found in the blood circulation of cancer patients believed to provide a means of diagnosing the likelihood for metastatic spread and assessing response to therapy in advanced, as well as early stage disease settings. Numerous technical efforts have been made to reliably detect and quantify CTCs, but the development of a universal assay has proven quite difficult. Notable challenges for developing a broadly useful CTC-based diagnostic assay are the development of easy-to-operate methods that 1) are sufficiently sensitive to reliably detect the small number of CTCs that are present in the circulation and 2) can capture the molecular heterogeneity of tumor cells. In this review, we describe recent progress towards the application of synthetic oligonucleotide aptamers as promising, novel, robust tools for the isolation and detection of CTCs. Advantages and challenges of the aptamer approach are also discussed.

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“…1-2 μg/mL), and other fluorescent aptasensors [53]. Notably, being compared to voltage current and electrochemical methods, homogeneous detection is robust, rapid and does not affect interacting surfaces of the biomolecules which can be detected without the need for additional steps and reagents [54,55].…”

Section: Fluorescent Oligonucleotides In Direct Sensing Of Autoantibomentioning

confidence: 99%

Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies

Astakhova¹

2014

J Clin Cell Immunol

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In this review, applications of synthetic oligonucleotide probes in diagnostics and studies of autoimmune antibodies against double-stranded DNA (anti-dsDNA) are described. As outlined herein, synthetic oligonucleotides and monoclonal antibodies provide appealing opportunities to develop standard simple assays for detection of anti-dsDNAs. Recent examples of anti-dsDNA detection using synthetic nucleic acid antigens include those applying surface plasmon resonance (SPR), antibody microarrays and homogeneous, or all-in-solution, detection with fluorescently-labelled probes. Since sequences of the applied nucleic acid antigens and monoclonal antibodies are known, the major benefit of these assays is the ability for the first time to clearly define the structural factors that govern the formation and stability of anti-dsDNA complexes. This is an essential first step toward understanding immune-complex mediated tissue injury in autoimmune conditions such as systemic lupus erythematosus (SLE) and arthritis, which involve production of pathogenic anti-dsDNAs. Moreover, synthetic nucleic acids and monoclonal antibodies have been demonstrated as key tools for the development of novel nucleic acid sensors for serotyping, along with studies of specificity and avidity of the antibody-dsDNA binding process. Among other antigens, fluorescent oligonucleotides prepared by click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides are proved to be very promising tools for efficient homogeneous detection of anti-dsDNAs.

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Detection of single tumor cell resistance with aptamer biochip

Cited by 24 publications

References 29 publications

Biochips, aplicaciones convencionales e innovación: Una revisión documental

Biochips, aplicaciones convencionales e innovación: Una revisión documental

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies

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