Detection of single walled carbon nanotube based sensors in a large mammal

Hofferber, Eric M.; Meier, Jakob; Herrera, Nicolas; Stapleton, Joseph A.; Calkins, Chris R.; Iverson, Nicole M.

doi:10.1016/j.nano.2021.102489

Cited by 20 publications

(22 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Single-walled carbon nanotubes (SWCNTs) benefit from unique optical and electronic properties, which render them favorable fluorescent probes for imaging, sensing, and biomedical applications, − owing to their fluorescence in the near-IR range where tissue, blood, and biological samples in general are mostly transparent. − Moreover, SWCNT sensors are stable at room temperature, provide spatiotemporal information, and do not photobleach upon use, unlike many other fluorescent sensors. − The mechanism of SWCNT-based sensors usually relies on tailored functionalization of the nanotube surface, which mediates the interaction with the analyte of interest, such that binding of the target molecule results in a modulation of the emitted fluorescence. − Fluorescent SWCNT sensors were applied for the biosensing of different analytes and enzymes. ,,,,− These range from monitoring progesterone and cortisol in vivo (mice), fibrinogen and insulin in blood and cell culture, , nitroaromatics and pathogens , in vivo (plants), volatiles in the gaseous phase, to enzymatic activity. − …”

Section: Introductionmentioning

confidence: 99%

Monitoring the Activity and Inhibition of Cholinesterase Enzymes using Single-Walled Carbon Nanotube Fluorescent Sensors

2022

View full text Add to dashboard Cite

Cholinesterase enzymes are involved in a wide range of bodily functions, and their disruption is linked to pathologies such as neurodegenerative diseases and cancer. While cholinesterase inhibitors are used as drug treatments for diseases such as Alzheimer and dementia at therapeutic doses, acute exposure to high doses, found in pesticides and nerve agents, can be lethal. Therefore, measuring cholinesterase activity is important for numerous applications ranging from the search for novel treatments for neurodegenerative disorders to the on-site detection of potential health hazards. Here, we present the development of a near-infrared (near-IR) fluorescent single-walled carbon nanotube (SWCNT) optical sensor for cholinesterase activity and demonstrate the detection of both acetylcholinesterase and butyrylcholinesterase, as well as their inhibition. We show sub U L–1 sensitivity, demonstrate the optical response at the level of individual nanosensors, and showcase an optical signal output in the 900–1400 nm range, which overlaps with the biological transparency window. To the best of our knowledge, this is the longest wavelength cholinesterase activity sensor reported to date. Our near-IR fluorescence-based approach opens new avenues for spatiotemporal-resolved detection of cholinesterase activity, with numerous applications such as advancing the research of the cholinergic system, detecting on-site potential health hazards, and measuring biomarkers in real-time.

show abstract

Section: Introductionmentioning

confidence: 99%

Monitoring the Activity and Inhibition of Cholinesterase Enzymes using Single-Walled Carbon Nanotube Fluorescent Sensors

2022

View full text Add to dashboard Cite

show abstract

“…Only functionalized, suspended SWCNTs reveal a fluorescence signal. Several studies demonstrated the feasibility of SWCNTs as fluorescence sensors or markers in vivo [ [46] , [47] , [48] , 51 , 59 , [68] , [69] , [70] , [71] , [72] , [73] , [74] , [75] , [76] ]. For example, single nanoparticle tracking of SWCNTs in the extracellular space of live brains could locally resolve its dimensions and viscosity [ 77 , 78 ].…”

Section: Introductionmentioning

confidence: 99%

“…For example, single nanoparticle tracking of SWCNTs in the extracellular space of live brains could locally resolve its dimensions and viscosity [ 77 , 78 ]. Moreover, a nitric oxide sensor for epidermal tissue inflammation was demonstrated in mice [ 42 ], and experiments with larger animal models are emerging [ 69 , 79 ].…”

Section: Introductionmentioning

confidence: 99%

In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window

Hendler‐Neumark

Wulf

Bisker

2021

Materials Today Bio

View full text Add to dashboard Cite

Caenorhabditis elegans ( C. elegans) nematodes serve as a model organism for eukaryotes, especially due to their genetic similarity. Although they have many advantages like their small size and transparency, their autofluorescence in the entire visible wavelength range poses a challenge for imaging and tracking fluorescent proteins or dyes using standard fluorescence microscopy. Herein, near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) are utilized for in vivo imaging within the gastrointestinal track of C. elegans . The SWCNTs are biocompatible, and do not affect the worms’ viability nor their reproduction ability. The worms do not show any autofluorescence in the NIR range, thus enabling the spectral separation between the SWCNT NIR fluorescence and the strong autofluorescence of the worm gut granules. The worms are fed with ssDNA-SWCNT which are visualized mainly in the intestine lumen. The NIR fluorescence is used in vivo to track the contraction and relaxation in the area of the pharyngeal valve at the anterior of the terminal bulb. These biocompatible, non-photobleaching, NIR fluorescent nanoparticles can advance in vivo imaging and tracking within C. elegans and other small model organisms by overcoming the signal-to-noise challenge stemming from the wide-range visible autofluorescence.

show abstract

“…[20,21] Semiconducting SWCNT fluoresce in the near-infrared (nIR) range, where absorption, scattering, and autofluorescence of biological tissues are reduced, rendering them favorable for biomedical sensing and imaging applications. [22][23][24][25][26][27][28][29][30][31][32][33] Being hydrophobic, SWCNTs can be suspended using noncovalent surface functionalization via hydrophobic interactions with amphiphilic molecules (polymer-lipid composites, surfactants), or via π-π stacking interactions, for example, with ssDNA. [23][24][25] The combination of a SWCNT and its surface functionalization forms unique 3D spatial and chemical structures, capable of providing rather specific reaction sites for molecular recognition, indicated through measurable alterations in the SWCNT fluorescence profile.…”

Section: Introductionmentioning

confidence: 99%

Oncometabolite Fingerprinting Using Fluorescent Single‐Walled Carbon Nanotubes

Amir

Hendler‐Neumark

Wulf

et al. 2021

Adv Materials Inter

View full text Add to dashboard Cite

These local changes in the levels of involved metabolites or products can indicate an ongoing process of a cancerous transformation, and even further contribute to its escalation, rendering them oncometabolites. [1][2][3][4][5][6][7][8][9] D-2-hydroxyglutarate (D2HG) is a product of the gain-of-function-mutated enzymes isocitrate dehydrogenase (IDH1/ IDH2), whereas the normal mitochondrial form plays a role in the tricarboxylic acid (TCA) cycle by producing α-ketoglutarate. [1,2,[4][5][6][7][8][9] Hence, an accumulation of D2HG in the mitochondria indicates a mutagenesis of isocitrate dehydrogenase (IDH) and interruption of the TCA cycle, implying that the cell is in a state of pseudo-hypoxia (resembling a state of low oxygen levels), and mainly relying on glycolysis for energy production (Scheme 1). [4] Normally, as a minor metabolic byproduct, D2HG levels are kept low as D-2-hydroxyglutarate dehydrogenase (D2HGDH) converts it to α-ketoglutarate. [1,10] However, D2HGDH does not prevent D2HG accumulation in the case of overproduction. [10] Moreover, a congenital loss of D2HGDH causes D2HG accumulation, which can lead to a severe brain damage in a rare condition called D-2-hydroxyglutarate aciduria. [1,10] The accumulation of D2HG was shown to inhibit a group of enzymes named a-ketoglutarate-dependent dioxygenases (aKGdd), resulting in the promotion of mutagenesis by epigenetic modification of DNA and histone hypermethylation. [1,4,5,7] Notably, IDH1-mutated tumors were shown to exhibit DNA hypermethylation in gliomas and leukemia, and also histone hypermethylation. [1,[5][6][7]9,11] Accumulation of 2-hydroxyglutarate (both D and L optical isomers) was found to suppress cell-proliferation regulation. [1,9] As a demonstration of D2HG cellular potency, it was shown that incubating hematopoietic stem cells with D2HG alters differentiation patterns and promotes immune resistance. [1,6] IDH mutations and D2HG accumulation were found in gliomas and glioblastomas, and D2HG accumulation is suspected to be sufficient for promoting the development of acute myeloid leukemia (AML). [1,6] Another possible oncogenic mechanism linked to D2HG abundance, is its ability to shift cellular redox equilibrium toward overproduction of reactive oxygen species (ROS), which are well-known oncogenic agents. [4] The same mechanism was also found to impair immune antitumor activity. [1,8] The production of oncometabolites is the direct result of mutagenesis in key cellular metabolic enzymes, appearing typically in cancers such as glioma, leukemia, and glioblastoma. Once accumulated, oncometabolites promote cancerous transformations by interfering with important cellular functions. Hence, the ability to sense and quantify oncometabolites is essential for cancer research and clinical diagnosis. Here, the authors present a near-infrared optical nanosensor for a known oncometabolite, D-2-hydroxyglutarate (D2HG), discovered in a screening of a library of fluorescent single-walled carbon nanotubes (SWCNTs) functionalized with ssDNA. The screening r...

show abstract

Detection of single walled carbon nanotube based sensors in a large mammal

Cited by 20 publications

References 23 publications

Monitoring the Activity and Inhibition of Cholinesterase Enzymes using Single-Walled Carbon Nanotube Fluorescent Sensors

Monitoring the Activity and Inhibition of Cholinesterase Enzymes using Single-Walled Carbon Nanotube Fluorescent Sensors

In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window

Oncometabolite Fingerprinting Using Fluorescent Single‐Walled Carbon Nanotubes

Contact Info

Product

Resources

About