Detection of small subendocardial infarction using speckle tracking echocardiography in a rat model

Bachner-Hinenzon, Noa; Shlomo, Liron; Khamis, Hanan; Ertracht, Offir; Vered, Zvi; Malka, Assaf; Binah, Ofer; Adam, Dan

doi:10.1111/echo.13291

Cited by 6 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[6][7][8] A strain analysis based on STI can be used to evaluate myocardial injury in various heart diseases in experimental rats. [9][10][11][12] Reports have confirmed its usefulness as a simple and accurate method of generating detailed local images of cardiac function. Since Torrent-Guasp 13 first proposed the theory of a continuous helical band of ventricular myocardial fibers in 1957, our understanding of the nature of myocardial motion has been continuously improved.…”

mentioning

confidence: 93%

“…Currently, 2‐dimensional speckle‐tracking imaging (STI), based on frame‐by‐frame tracking of myocardial motion, is a relatively new method of angle‐independent quantification of left ventricular function . A strain analysis based on STI can be used to evaluate myocardial injury in various heart diseases in experimental rats . Reports have confirmed its usefulness as a simple and accurate method of generating detailed local images of cardiac function.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Experimental Research on the Evaluation of Left Ventricular Function by Layered Speckle Tracking in a Constrictive Pericarditis Rat Model

Wang

Qiao

et al. 2020

J of Ultrasound Medicine

View full text Add to dashboard Cite

Objectives-In animal models with constrictive pericarditis (CP), detecting the function of cardiac systole by conventional noninvasive ultrasound is a challenge. We aimed to detect cardiac dysfunction in rat models with CP in the early stage by layered speckle tracking. Methods-We compared a rat CP model (n = 23, injected with a solution of 1-mg/mL lipopolysaccharides [0.5 mL] and a 10% talc suspension [0.5 mL]) with a control group (n = 20, no injection). After 8 weeks, conventional echocardiography and layered speckle tracking were used to assess the left ventricular structures and functions in the groups. Results-The global circumferential strain (CS) and longitudinal strain (LS) were decreased in the CP group (P < .05). The CS of the epicardial and middle layers in the CP group was decreased (P < .05), but the endocardial layer was not statistically different. The LS of the epicardial layer was decreased (P < .05), but the middle and endocardial layers were not statistically different. The global free-wall and septal-wall CS of the CP group was decreased (P < .05), mainly due to the decrease of CS of the epicardial and middle layers. The global free-wall LS of the CP group was decreased (P < .05), mainly due to the decrease of LS of the epicardial and middle layers. There were no significant differences between the groups in global LS of the septal wall. Conclusions-In the early stage of CP, subepicardial myocardial damage precedes that of the subendocardial myocardium, and free-wall damage precedes that of the septal wall.

show abstract

mentioning

confidence: 93%

mentioning

confidence: 99%

Experimental Research on the Evaluation of Left Ventricular Function by Layered Speckle Tracking in a Constrictive Pericarditis Rat Model

Wang

Qiao

et al. 2020

J of Ultrasound Medicine

View full text Add to dashboard Cite

show abstract

“…Rats were sedated and exposed shaved chests. Rats were placed in a left lateral decubitus position and scanned by a Vivid-i™ ultrasound system (GE Healthcare Life Sciences, Pittsburgh, PA, USA) using a 10S transducer and a cardiac application with 10 MHz, 2.5 cm depth and 225-350 fps of transmission frequency as previously described [12]. The measurements included short-axis cross sections of the apex and papillary muscles levels.…”

Section: Echocardiographymentioning

confidence: 99%

AMPK/mTOR-mediated therapeutic effect of metformin on myocardial ischemia reperfusion injury in diabetic rat

Zhang¹,

Zhang²,

Guan³

et al. 2020

Preprint

View full text Add to dashboard Cite

BACKGROUND: The autophagy associated signalling pathways such as AMPK/mTOR previously were suggested to play a crucial role in protecting from ischemia-reperfusion injury (IRI). The objective of this study was to evaluate the effect of metformin (DMBG) on autophagy during myocardial IRI with diabetes mellitus (DM). METHODS: The DM rat model was established using streptozocin, and further induced ischemia model via transitory ligation of the left anterior coronary artery and following reperfusion. The model rats were treated with 400 mg/kg/day DMBG for one week. Autophagosomes were investigated using transmission electron microscopy. Autophagy-associated signalling pathways were detected by western blot. RESULTS: The myocardial infarct size was shown to significantly increase in the DM rats exposed to IRI compared to negative control, but decrease in DMBG treated. The mature autophagosomes were elevated in infarction and marginal zones of DM+IRI+DMBG compared to DM+IRI. Furthermore, the increasing protein levels of LC3-II, BECLIN 1, autophagy related 5 (ATG5) and AMP-activated protein kinase suggested activated autophagy-associated intracellular signalling AMPK and mTOR pathways upon DMBG treated. CONCLUSIONS: Taken together, the outcomes demonstrated that DMBG could activate autophagy process to provide a cardio-protective effect against DM induced myocardial IRI.

show abstract

“…Some animal model experiments suggest that small non-transmural infarcts are surrounded by tissue that is contracting normally. 25,26 This contracting tissue may pull the infarcted tissue inward during systole, making the infarcted region appear to contract normally and so remain undetected in real-time assessment early after the infarction. The high resolution of strain estimation using 2DSTE may have substantial potential in clinical practice, as the ability to assess the size, location, and transmural extent of an infarct is related to the accuracy and resolution of the strain estimation.…”

Section: Introductionmentioning

confidence: 99%

Parametrization of subsegmental infarcts using high spatial resolution 2DSTE and synthetic ultrasonic data

Żmigrodzki,

Cygan,

Kałużyński

2023

Polish Journal of Medical Physics and Engineering

View full text Add to dashboard Cite

Introduction: The purpose of this study was to assess the performance of a set of parameters in characterizing simulated infarcts in left ventricular (LV) models with variations in size and transmural extent. Material and methods: The deformation of the LV models with different infarct sizes was simulated using the Finite Element Method. These simulations provided meshes that were used to generate synthetic ultrasonic data within the FIELD II package. The strain components (longitudinal and circumferential) were then estimated over small subsegments of the of segments 7 and 12 (according to 17-segment left ventricle segmentation standard proposed by the American Heart Association - AHA17), using a hierarchical block matching method. The strain maps obtained were utilized to calculate the Strain Drop Factor (SDF) maps, which represent the percentage ratio of strain observed in the subsegments of the studied model to that observed in the healthy model. Infarct segmentation was performed using these maps, and various parameters were derived, including Infarct Cross-Section Area (ICSA), relative ICSA, Transmurality Ratio (TR), Mean Infarct Transmurality (MIT), strain drop factor in the infarcted region (SDFi), and Strain Contrast (SC). Results: The estimates of ICSA, SC, MIT, and SDFI showed good repeatability and demonstrated the ability to provide a quantitative assessment of the size and transmural extent of the infarcts. Conclusions: The study findings suggest that the evaluated parameters, including ICSA, SC, MIT, and SDFI, can be reliably used to assess the size and transmural extent of infarcts. These parameters offer a quantitative approach for characterizing infarcts based on strain analysis and have the potential to contribute to the diagnosis and evaluation of myocardial infarctions.

show abstract

Detection of small subendocardial infarction using speckle tracking echocardiography in a rat model

Abstract: The TTP of the CS can distinguish between regions adjacent to MI (stunned or tethered) and MI, even in small nontransmural infarcts.

Cited by 6 publications

References 35 publications

Experimental Research on the Evaluation of Left Ventricular Function by Layered Speckle Tracking in a Constrictive Pericarditis Rat Model

Experimental Research on the Evaluation of Left Ventricular Function by Layered Speckle Tracking in a Constrictive Pericarditis Rat Model

AMPK/mTOR-mediated therapeutic effect of metformin on myocardial ischemia reperfusion injury in diabetic rat

Parametrization of subsegmental infarcts using high spatial resolution 2DSTE and synthetic ultrasonic data

Contact Info

Product

Resources

About