Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer

Li, Wen-han; Zhang, Hao; Guo, Qi; Wu, Xuandi; Xu, Zisen; Dang, Chengxue; Xia, Peng; Song, Yi

doi:10.1155/2015/657570

Cited by 50 publications

(45 citation statements)

References 30 publications

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“…Previous studies have shown that expression of SNCA is regulated by hypermethylation of the CpG island in its promoter region in various human cancers . Demethylation drug treatment in MB cells showed reversed expression of this gene, suggesting that the silenced expression of SNCA might be due to epigenetic regulation, including DNA hypermethylation (Figure A).…”

Section: Resultsmentioning

confidence: 83%

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SNCA, a novel biomarker for Group 4 medulloblastomas, can inhibit tumor invasion and induce apoptosis

Jiang

et al. 2018

Cancer Science

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Medulloblastoma (MB) is the most common malignant brain tumor in childhood. It contains at least four distinct molecular subgroups. The aim of this study is to explore novel diagnostic and potential therapeutic markers within each subgroup of MB, in particular within Group 4, the largest subgroup, to facilitate diagnosis together with gene therapy. One hundred and six MB samples were examined. Tumor subtype was evaluated with the NanoString assay. Several novel tumor related genes were shown to have high subgroup sensitivity and specificity, including PDGFRA,FGFR1, and ALK in the WNT group, CCND1 in the SHH group, and α‐synuclein (SNCA) in Group 4. Knockdown and overexpression assays of SNCA revealed the ability of this gene to inhibit tumor invasion and induce apoptosis. Methylation‐specific PCR and pyrosequencing analysis showed that epigenetic mechanisms, rather than DNA hypermethylation, might play the key role in the regulation of SNCA expression in MB tumors. In conclusion, we identify SNCA as a novel diagnostic biomarker for Group 4 MB. Some other subgroup signature genes have also been found as candidate therapeutic targets for this tumor.

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Section: Resultsmentioning

confidence: 83%

“…It has been reported that SNCA expression is regulated by epigenetic mechanisms in a series of human cancers, including cholangiocarcinoma, colorectal cancer, and non‐Hodgkin's lymphoma . Bethge et al reported that SNCA methylation might be suitable for early detection and monitoring of non‐Hodgkin's lymphoma.…”

Section: Discussionmentioning

confidence: 99%

SNCA, a novel biomarker for Group 4 medulloblastomas, can inhibit tumor invasion and induce apoptosis

Jiang

et al. 2018

Cancer Science

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“…In order to improve the efficiency of cell wall lysis of gram-positive bacteria in the feces, the samples were incubated at 95°C for 5 minutes. The extracted DNA was stored at -20°C until processed (24).…”

Section: Dna Extraction 16s Rrna Gene Amplification and Sequencingmentioning

confidence: 99%

Characterization of Fecal Microbiota, Short-Chain Fatty Acids and Lactic Acid Concentrations in 5 - 8-Year-Old Children with Cow Milk Protein Allergy

Dong

Peng

Han³

et al. 2018

Iran J Pediatr

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Background: Cow milk protein allergy (CMPA) has been reported to be associated with the alterations of gut microbiota. However, changes in the fecal microbial community for children aged 5 -8 years with CMPA have not been clearly elucidated. Objectives: The aim of the study is to assess the differences and relationship between fecal microbiota and levels of fecal short-chain fatty acids (SCFAs) and lactic acid in 5 -8-year-old children with CMPA, compared with the normal children. Methods: We enrolled 6 CMPA children and 8 healthy children from Harbin children's hospital (Heilongjiang, China). Total bacterial DNA of all fecal samples was extracted and prepared for bioinformatics analysis by 16S rDNA amplicon sequencing. Lactic acids and SCFAs were measured by the high-performance liquid chromatography (HPLC) technique. Results: Compared with healthy individuals, the fecal microbial community of CMPA children showed a significant increment of Firmicutes and depletion of Proteobacteria (P < 0.05). At the class level, the number of Clostridia was observed to be significantly increased in the CMPA children's fecal microbiota. Meanwhile, the diversity of Ruminococcaceae and Subdoligranulum was higher in the fecal microbiome of CMPA children than in healthy children (P < 0.05). Furthermore, linear discriminant analysis of effect size (LEfSe) revealed some significantly enriched taxa of CMPA children and control subjects. There are 40 and 5 enriched taxa in the fecal microbiota of children with CMPA and healthy children respectively. The concentration of total SCFAs was lower in feces of CMPA children than in healthy children. Lactic acid had a significantly negative correlation with the abundance of Bacteroides. Conclusions: Alterations in the composition of fecal microbiota and SCFAs in the 5 -8-year-old children with CMPA may reduce immune function and potentially promote this allergy.

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“…The discovery of the presence of DNA in fecal and urine samples [46] and circulating nucleosomes in serum [47,48] has led to the foundation of identifying epigenetic markers such as DNA methylation and histone posttranslational modification for cancer diagnosis. Ahlquist et al [49] demonstrated the recovery of DNA from frozen fecal samples of colorectal cancer patients which was followed by other investigators showing matching DNA methylation patterns between DNA from tissue and fecal samples of gastric and colorectal cancer patients [50][51][52] . Methylation pattern of DNA isolated from urine samples was also used to diagnose bladder and prostate cancer [53][54][55][56][57] .…”

Section: Histone Ptms In Cancermentioning

confidence: 88%

Global histone post-translational modifications and cancer: Biomarkers for diagnosis, prognosis and treatment?

Khan

Reddy

Gupta

2015

WJBC

107

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Global alterations in epigenetic landscape are now recognized as a hallmark of cancer. Epigenetic mechanisms such as DNA methylation, histone modifications, nucleosome positioning and non-coding RNAs are proven to have strong association with cancer. In particular, covalent post-translational modifications of histone proteins are known to play an important role in chromatin remodeling and thereby in regulation of gene expression. Further, histone modifications have also been associated with different aspects of carcinogenesis and have been studied for their role in the better management of cancer patients. In this review, we will explore and discuss how histone modifications are involved in cancer diagnosis, prognosis and treatment.

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Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer

Cited by 50 publications

References 30 publications

SNCA, a novel biomarker for Group 4 medulloblastomas, can inhibit tumor invasion and induce apoptosis

SNCA, a novel biomarker for Group 4 medulloblastomas, can inhibit tumor invasion and induce apoptosis

Characterization of Fecal Microbiota, Short-Chain Fatty Acids and Lactic Acid Concentrations in 5 - 8-Year-Old Children with Cow Milk Protein Allergy

Global histone post-translational modifications and cancer: Biomarkers for diagnosis, prognosis and treatment?

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