Detection of soluble ERBB2 in breast cancer cell lysates using a combined label-free/fluorescence platform based on Bloch surface waves

Sinibaldi, Alberto; Sampaoli, Camilla; Danz, Norbert; Munzert, Peter; Sibilio, Leonardo; Sonntag, Frank; Occhicone, Agostino; Falvo, Elisabetta; Tremante, Elisa; Giacomini, Patrizio; Michelotti, Francesco

doi:10.1016/j.bios.2017.02.012

Cited by 44 publications

(37 citation statements)

References 34 publications

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“…Physiological levels of HER2 in the blood of healthy people range from 4 to 14 ng mL −1 , while its concentration in the blood of cancer patients is higher (14–75 ng mL −1 ). HER2 is over‐expressed in around 20–30 % of breast cancer patients and is also associated with an aggressive form of the disease and a poor prognosis ,. Higher levels of HER2 were also observed during gastric, oesophageal, ovarian and endometrial cancers.…”

Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

et al. 2018

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affinity biosensors are, without any doubt, among the most sensitive analytical devices available, offering low limits of detection and wide linear response ranges. There are, however, only a few papers detailing the application of impedimetric biosensors for the analysis of clinically relevant samples with due clinical performance. The fact that these devices have not found their way to any commercial or clinical use to date might be surprising, since an electrochemical assay platform based on portable potentiostats is a success story for monitoring a range of clinical parameters such as ions, haematological indicators and glucose. This review discusses the reasons behind this discrepancy and addresses the barriers to be overcome in order to achieve the point-of-care diagnostics using such devices for detection of protein oncomarkers approved by FDA. The final part of the review covers the most recent progress in the area.[a] Dr.

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Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

et al. 2018

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“…The commercialisation of several waveguide-based sensors has spurred the development of a profuse variety of biosensors for several types of clinically relevant biomolecules [156]. Evanescent sensors have been exploited for recognition of complex biomolecules as well as smaller analytes [158]. Optical fibres have been the substrate of choice to develop these sensors as they are compatible with several different classes of receptors.…”

Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

“…The sensor was capable of quantifying CRP down to 581.3 zg mL −1 which was 8 times lower than conventional sandwich ELISA [169]. Evanescent sensors have been exploited for recognition of complex biomolecules as well as smaller analytes [158]. Optical fibres have been the substrate of choice to develop these sensors as they are compatible with several different classes of receptors.…”

Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

2020

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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“…In spite of sensitivities to local refractive index (RI) changes lower than SPR platforms, the completely dielectric low‐loss structure of a BSW sensor yields sensibly sharper resonances, leading to generally larger FOMs . This has recently led to the successful development of label‐free biosensors exhibiting competitive performance in practical biomolecular detections . Moreover, BSW structures offer further benefits with respect to other SW‐based counterparts, such as easy realization and experimental observation, and great flexibility in terms of wavelength range of operation, materials choice, and tailoring of the field distribution for specific sensing applications …”

Section: Introductionmentioning

confidence: 99%

“…While most sensing platforms proposed in the literature rely on bulky prism‐coupled planar configurations, the integration of BSW structures with optical fibers would bring significant benefits in terms of compactness, light weight, remote sensing capability, biocompatibility, and ease of interrogation. This is especially attractive within the emerging “lab‐on‐fiber” technology framework .…”

Section: Introductionmentioning

confidence: 99%

Excitation of Bloch Surface Waves on an Optical Fiber Tip

Scaravilli

Micco

Castaldi

et al. 2018

Advanced Optical Materials

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to the development of extremely sensitive surface plasmon resonance (SPR) biosensors, which can rely on mature fabrication technologies, standard biofunctionalization protocols, and commercial scale production. [1,[3][4][5] In spite of the excellent performance achieved by state-of-the-art SPR platforms, with reported limits of detection (LODs) approaching 10 −7 refractive index units (RIU), [4,6] further improvements are unavoidably limited by the reliance on lossy materials (typically metals). This yields an undesirable resonance broadening, thereby posing an upper bound to the overall figure of merit (FOM). [6] For this reason, there is a strong push to explore alternative, larger-FOM SW implementations. [1,[7][8][9][10][11] As a prominent example, Bloch SWs (BSWs) on truncated 1D photonic crystals (1DPCs) represent an attractive alternative to SPPs, as recently demonstrated in several proof-of-principle studies. [9,[12][13][14][15][16] In spite of sensitivities to local refractive index (RI) changes lower than SPR platforms, the completely dielectric low-loss structure of a BSW sensor yields sensibly sharper resonances, leading to generally larger FOMs. [13][14][15][16][17] This has recently led to the successful development of label-free biosensors exhibiting competitive performance in practical biomolecular detections. [18][19][20][21][22][23][24][25] Moreover, BSW structures offer further benefits with respect to other SW-based counterparts, such as easy realization and experimental observation, and great flexibility in terms of wavelength range of operation, materials choice, and tailoring of the field distribution for specific sensing applications. [1,9,[13][14][15]17,[26][27][28] While most sensing platforms proposed in the literature rely on bulky prism-coupled planar configurations, [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] the integration of BSW structures with optical fibers would bring significant benefits in terms of compactness, light weight, remote sensing capability, biocompatibility, and ease of interrogation. This is especially attractive within the emerging "labon-fiber" technology framework. [29][30][31][32][33][34] The interest in this topic has started growing within the last two years, as witnessed by theoretical studies of BSW sensors based on the use of D-type fibers, [35] and unclad regions of multimode fibers. [36] A first experimental demonstration on a tapered single-mode fiber has also been provided very recently. [37] Within this context, a more intriguing scenario is represented by the integration of the resonant structure directly on the fiber tip. This would allow exploiting fabrication methodologies typically adopted for photonic biochips, thereby enabling the development of probes The integration of structures supporting Bloch surface waves (BSWs) with optical fibers is highly desirable, since it would enable the development of high-figure-of-merit miniaturized all-fiber optrodes, opening new pathways within the "lab-on-fiber" roadmap. Here, the first expe...

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Detection of soluble ERBB2 in breast cancer cell lysates using a combined label-free/fluorescence platform based on Bloch surface waves

Cited by 44 publications

References 34 publications

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

Recent Progress in Optical Sensors for Biomedical Diagnostics

Excitation of Bloch Surface Waves on an Optical Fiber Tip

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