Detection of somatotropin receptors on human monocytes

Jafari, Parviz; Khansari, David N.

doi:10.1016/0165-2478(90)90048-u

Cited by 12 publications

(3 citation statements)

References 17 publications

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“…These results fully agreed with the observed Bcl-2 up-regulation induced by hGH and its subsequent protective effect on Fas-induced cell death. We thus demonstrated that upon ligation to its receptors, which is also present on human monocytes [39] and on U937 cells (our unpublished results), hGH specifically promotes a cascade of biochemical events leading to a down-regulation of Fas-induced caspase activation. Interestingly, preliminary experiments reveal that, despite the presence of hGH receptors on T cells [40], no protection was observed in human peripheral T lymphocytes activated with anti-CD3 mAb and IL-2 and treated with anti-Fas mAb in the presence of up to 1 ?…”

Section: Discussionsupporting

confidence: 56%

Growth hormone prevents human monocytic cells from Fas-mediated apoptosis by up-regulating Bcl-2 expression

et al. 1999

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Apoptosis and particularly Fas-mediated apoptosis has been proposed to play a key role in controlling monocyte homeostasis. We and others have documented the regulatory function of human growth hormone (hGH) on monocytic cells, which prompted us to investigate the role of hGH on their response to Fas antigen cross-linking. Using human promonocytic U937 cells constitutively producing hGH upon gene transfer and human primary monocytes cultured in the presence of recombinant hGH, we demonstrated that hGH diminished Fasmediated cell death by enhancing the expression of the antiapoptotic oncoprotein Bcl-2 as well as the level of bcl-2 § mRNA. In parallel, we established that overexpression of Bcl-2 through gene transfer into normal U937 cells also diminished Fas-induced apoptosis. Further, as a result of Bcl-2 overexpression, we found that hGH greatly depressed Fas-induced activation of the cysteine protease caspase-3 (CPP32), which in turn affected the cleavage of poly(ADP-ribose) polymerase. Altogether, these data provide evidence that hGH mediates its protective effect through a Bcl-2-dependent pathway, clearly a crucial step in enhanced survival of monocytic cells exposed to Fas-induced death.

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Section: Discussionsupporting

confidence: 56%

Growth hormone prevents human monocytic cells from Fas-mediated apoptosis by up-regulating Bcl-2 expression

et al. 1999

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“…These results may relate to an increase in the number of hGH receptors expressed by activated monocytes, in which case the maximal level of hGH stimulatory effects would be observed for a high hGH concentration in conjunction with a greater number of binding sites on the surfaces of cell membranes. However, few studies 21,22 have considered the expression of hGH receptors on the surface of the monocyte/macrophage lineage, and none has investigated the expression of GH receptors after LPS activation of monocytes. Moreover, as studies concerning the role of hGH as a macrophage-activating factor are in the early stages, several years of research will be required for thorough investigation of hGH receptor expression by phagocytic cells in response to cytokines or soluble factors implicated in inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

“…Growth hormone (GH), a key substance affecting bone metabolism through stimulation of osteoblasts [15][16][17] and regulation of osteoclastic resorption, 18 also has been considered to be a factor in the regulation of immune system function 19 and hematopoiesis. 20 GH receptors have been found on lymphocytes, natural killer cells, and monocytes/macrophages 21,22 whereas mononuclear leukocytes and monocytes apparently can express GH mRNA and release GH in culture. 23 These findings indicate that GH possesses endocrine, autocrine, and paracrine mechanisms of action.…”

Section: Introductionmentioning

confidence: 99%

Growth hormone stimulates the degradation of calcium phosphate biomaterial by human monocytes macrophagesin vitro

Guicheux

Kimakhe

Heymann

et al. 1998

J. Biomed. Mater. Res.

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This study investigated the effects of human growth hormone (hGH) on the monocyte/macrophage lineage, the first cell population involved in degradation of calcium phosphate ceramic after in vivo implantation. Monocytes isolated from human blood were cultured on biphasic calcium pellets (200 mg) for 8 days in the presence of lipopolysaccharides (LPS, 0.5 microgram/mL), hGH (10 and 50 ng/mL), or an association of LPS with hGH (10 and 50 ng/mL). Unlike LPS, hGH significantly decreased (about 25%) the total number of lacunae formed by monocytes. However, hGH induced the formation of lacunae with a greater surface area (about a 90% increase) as compared to the control. Finally, intense upmodulation (about a 250% increase) of lacuna surface area was observed in the presence of both soluble factors, suggesting that hGH and LPS act synergistically. In view of the development of a drug delivery system for hGH bone release, this study shows that hGH not only stimulates bone cells implicated in the synthesis of the extracellular matrix but also those involved in the early degradation of calcium phosphate biomaterial.

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Hormonal Interactions Between the Pituitary and Immune Systems

Berczi¹

1994

Bilateral Communication Between the Endocrine and Immune Systems

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Detection of somatotropin receptors on human monocytes

Cited by 12 publications

References 17 publications

Growth hormone prevents human monocytic cells from Fas-mediated apoptosis by up-regulating Bcl-2 expression

Growth hormone prevents human monocytic cells from Fas-mediated apoptosis by up-regulating Bcl-2 expression

Growth hormone stimulates the degradation of calcium phosphate biomaterial by human monocytes macrophagesin vitro

Hormonal Interactions Between the Pituitary and Immune Systems

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