Detection of some Heavy Metals Using Rhodamine B Isothiocyanate Fluoresceinated Dye

Abstract

The spectral behavior of the binary complexes of Rhodamine B Isothiocyanate (RBITC) dye with metal ions in aqueous medium is studied. The observed fluorescence quenching of the dye reveals that the binding constant for RBITC dye-metal ions follow the order Cu 2+ > Ni 2+ > Pb 2+ . Also, the spectral changes of the interaction of the studied dye with metal ions in presence of ACES zwitterionic buffer (ternary complexes) is carried out, where the interaction of dye with ACES zwitterionic buffer is accompanied by as light increasing of the peak intensity at λ= 585 nm and the binding constant for dye-zwitterionic buffer-metal ion systems were determined where the order of binding with metal ions is as follows: Pb 2+ > Ni 2+ > Cu 2+ .