Detection of specific antibodies against deflagellated Salmonella Enteritidis and S. Enteritidis FliC-specific 9kDa polypeptide

Mizumoto, Naoe; Toyota-Hanatani, Yukiko; Sasai, Kazumi; Tani, Hiroyuki; Ekawa, Tomoya; Ohta, Hiroaki; Baba, Eiichiroh

doi:10.1016/j.vetmic.2003.11.009

Cited by 14 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While mice immunized intraperitoneally with flagellated Salmonella show a strong systemic (immunoglobulin G IgG) response against flagellin, mice immunized mucosally with the strain did not (34). Nevertheless, flagella elicit a strong immune response in chickens (27,44) and are useful serological markers that carry the serotype-specific H-antigenic determinants (17) in the central variable domain of the protein (41). Therefore, deletion of fliC, the gene that codes for flagellin (FliC) should allow serological differentiation between animals immunized with the ⌬fliC vaccine strain and animals infected by wild-type S. enterica serovar Enteritidis.…”

mentioning

confidence: 99%

A Live Salmonella enterica Serovar Enteritidis Vaccine Allows Serological Differentiation between Vaccinated and Infected Animals

et al. 2007

View full text Add to dashboard Cite

Three precisely defined deletion mutants of Salmonella enterica serovar Enteritidis were constructed, a guanine auxotrophic ⌬guaB mutant, a nonflagellated ⌬fliC mutant, and an auxotrophic and nonflagellated ⌬guaB ⌬fliC double mutant. All three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line T84 and less efficiently internalized in the chicken macrophage cell line HD11. The ⌬fliC mutant was pathogenic in orally infected BALB/c mice, while the ⌬guaB mutant was attenuated and conferred protection against a challenge with the pathogenic parent strain. The ⌬guaB ⌬fliC double mutant was totally asymptomatic and conferred better protection than the ⌬guaB mutant. This indicates that the major flagellar protein flagellin is not required for efficient vaccination of BALB/c mice against Salmonella infection. The ⌬guaB ⌬fliC mutant was also safe for vaccination of 1-day-old chickens. After two immunizations, it induced statistically significant protection against infection of the internal organs of the birds by a virulent S. enterica serovar Enteritidis challenge strain but not against intestinal colonization. These data demonstrate that nonflagellated attenuated Salmonella mutants can be used as marker vaccines.

show abstract

mentioning

confidence: 99%

A Live Salmonella enterica Serovar Enteritidis Vaccine Allows Serological Differentiation between Vaccinated and Infected Animals

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Thus, the responsive antibody was considered to be IgM antibody. In another our report, a 53 kDa band was not detected in 4-week-old SPF chickens and 300-day-old field chickens, which received SE administration, but was detected in molting-induced chickens (Mizumoto N et al 2004, Piao Z et al 2007). Thus, the antibody against the 53 kDa polypeptide after SE inoculation is suspected no invasion into the internal organs.…”

Section: Active Component Of Inactivated Se Vaccine (Main Fli C Antigmentioning

confidence: 62%

“…Since SE infection causes no direct economic damage, vaccine administration to chickens on farms requires the high-level motivation of vaccine users. The first inactivated SE vaccine was a new type of vaccine, i.e., emergence of a high-cost vaccine slightly stressful to vaccinated animals and not preventing disease in the animals white leghorn chickens (Mizumoto N et al 2004). …”

Section: History Of Se Vaccinementioning

confidence: 99%

“…Detection of specific antibodies in sera against SE cell antigen and SEp9 on oral SE administration to field white leghorn chickens) (Mizumoto N et al 2004). (Mizumoto N et al 2004, Sunagawa H et al 1997, Yamane Y et al 2000 * Age of vaccination: around 80 days old ** ELISA: Indirect method with SE cell antigen coated. RPA: rapid plate agglutination with diagnostic for pullorum disease antigen.…”

Section: Risk Of Misjudging Inactivated Se Vaccine-treated Chickens Amentioning

confidence: 99%

“…The determination with specific antibody to g.m. antigen was done according to the method described by Mizumoto N et al 2004. The mean positive rate of the farms was about 88%.…”

mentioning

confidence: 99%

See 2 more Smart Citations