Detection of specific IgG, IgM, IgE and IgG subclass antibodies for serological diagnosis of human cystic echinococcosis

Abstract

SummaryAn enzyme linked immunosorbent assay (ELISA), based on sheep hydatid cyst fl uid antigen was used for the detection of specifi c antibodies of IgG, IgM, IgE and IgG subclass in the serum samples of 62 clinically and radiologically diagnosed cystic echinococcosis (CE) patients, 8 clinically suspected cases of CE, 25 other parasitic disease controls and 25 healthy controls. The diagnostic sensitivity in the clinically and radiologically suggestive cases (n = 62) for IgG antibody detection was highest (93 %), followed by IgE, IgG4, IgG1, IgG2, IgM and IgG3 with 89 %, 87 %, 85 %, 76 %, 70 % and 55 % respectively. The detection of specifi c IgE, IgG1 and IgG4 antibody showed the higher diagnostic sensitivity and specifi city to the extent that they can be safely used as better substitute to IgG. Even though, the diagnostic sensitivity of IgG was highest (93%) but was less specifi c (88 %) due to the frequent non-specifi c reactions in the sera of patients with other parasitic infections and healthy controls. None of the sera samples from healthy controls gave non-specifi c reaction with IgE, IgG1 and IgG4 and there was a considerably reduced cross-reaction with these antibodies. The most discriminatory and specifi c antibodies found in this study belonged to IgE, IgG1 and IgG4; therefore, these antibodies may serve as useful diagnostic markers for CE.

Pyogenic liver abscess in a child with concomitant infections –Staphylococcus aureus, Echinococcus multilocularisandMycobacterium tuberculosis

“…Humans acquire infection after the accidental ingestion of E. multilocularis eggs and subsequently larval stages proliferate primarily in the liver, but can also spread into extrahepatic structures and even metastasize to other organs. The clinical diagnosis is based on clinical fi ndings, lesion morphology shown by imaging techniques, serology, histopathology and molecular nucleic acid detection (Naik et al, 2015;Szilágyová et al, 2015). Untreated disease is usually considered lethal, as no curative treatment is available, except surgical resection of metacestode in an early stage of disease (Kern et al, 2006).…”