Detection of Streptococcus pyogenes Virulence Factors

Kozińska, Aleksandra; Sitkiewicz, Izabela

doi:10.1007/978-1-0716-0467-0_1

Cited by 3 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C5a peptidase and its unique target specificity were first identified in GBS [ 17 , 27 , 28 ], where an ability to bind fibronectin was later reported and postulated to contribute to mucosal colonization [ 28 ]. However, the roles of C5a peptidase in immune evasion and systemic infection have been more extensively studied in GAS [ 14 , 29 , 30 , 31 ]. In the present study, we further investigated the role of ScpB in GBS virulence and explored the biological significance of its homology with ScpA.…”

Section: Discussionmentioning

confidence: 99%

The Group A Streptococcal Vaccine Candidate VAX-A1 Protects against Group B Streptococcus Infection via Cross-Reactive IgG Targeting Virulence Factor C5a Peptidase

McCabe,

Bjånes,

Hendriks

et al. 2023

Vaccines

View full text Add to dashboard Cite

Group B Streptococcus (Streptococcus agalactiae or GBS) is the leading infectious cause of neonatal mortality, causing roughly 150,000 infant deaths and stillbirths annually across the globe. Approximately 20% of pregnant women are asymptomatically colonized by GBS, which is a major risk factor for severe fetal and neonatal infections as well as preterm birth, low birth weight, and neurodevelopmental abnormalities. Current clinical interventions for GBS infection are limited to antibiotics, and no vaccine is available. We previously described VAX-A1 as a highly effective conjugate vaccine against group A Streptococcus that is formulated with three antigens, SpyAD, streptolysin O, and C5a peptidase (ScpA). ScpA is a surface-expressed, well-characterized GAS virulence factor that shares nearly identical sequences with the lesser studied GBS homolog ScpB. Here, we show that GBS C5a peptidase ScpB cleaves human complement factor C5a and contributes to disease severity in the murine models of pneumonia and sepsis. Furthermore, antibodies elicited by GAS C5a peptidase bind to GBS in an ScpB-dependent manner, and VAX-A1 immunization protects mice against lethal GBS heterologous challenge. These findings support the contribution of ScpB to GBS virulence and underscore the importance of choosing vaccine antigens; a universal GAS vaccine such as VAX-A1 whose formulation includes GAS C5a peptidase may have additional benefits through some measure of cross-protection against GBS infections.

show abstract

Section: Discussionmentioning

confidence: 99%

The Group A Streptococcal Vaccine Candidate VAX-A1 Protects against Group B Streptococcus Infection via Cross-Reactive IgG Targeting Virulence Factor C5a Peptidase

McCabe,

Bjånes,

Hendriks

et al. 2023

Vaccines

View full text Add to dashboard Cite

show abstract

“…Antibiotic resistance of bacteria became a serious threat to human health decades ago [1], decreasing the potential of antimicrobial therapies. To date, however, antimicrobial agents remain the most popular tool in fight with microbial infections, as the alternative methods of treatment are scarcely developing [2]. Thus, the monitoring of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment is still needed, as it may help to tracking the resistance patterns and contribute to better understanding of resistance phenomenon, as well as to indicate possible ways to deal with this worldwide problem [3,4].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance in tap water during the summer season – preliminary research

Siedlecka

Piekarska

2019

E3S Web Conf.

View full text Add to dashboard Cite

Antibiotic resistance of bacteria is regarded as a global health risk and should be monitored in all environments, including tap water distribution systems. In this study, tap water samples were collected from two water treatment plants and selected points-of-use from the water distribution network. The abundances of antibiotic resistant bacteria were determined via culture-dependent method and the presence of selected antibiotic resistance genes was detected via PCR. The influence of the distance of points-of-use from water treatment plants on bacterial loads and antibiotic resistance phenomenon was evaluated. The paper presents preliminary results of a large-scale study concerning spatial and seasonal variation in antibiotic resistance of bacteria dwelling in the tap water system in Wrocław.

show abstract

Design, Characterization, and Biological Activities of Erythromycin-Loaded Nanodroplets to Counteract Infected Chronic Wounds Due to Streptococcus pyogenes

Mandras

Luganini

Argenziano

et al. 2023

IJMS

View full text Add to dashboard Cite

Streptococcus pyogenes causes a wide spectrum of diseases varying from mild to life threatening, despite antibiotic treatment. Nanoparticle application could facilitate the foreign pathogen fight by increasing the antimicrobial effectiveness and reducing their adverse effects. Here, we designed and produced erythromycin-loaded chitosan nanodroplets (Ery-NDs), both oxygen-free and oxygen-loaded. All ND formulations were characterized for physico-chemical parameters, drug release kinetics, and tested for biocompatibility with human keratinocytes and for their antibacterial properties or interactions with S. pyogenes. All tested NDs possessed spherical shape, small average diameter, and positive Z potential. A prolonged Ery release kinetic from Ery-NDs was demonstrated, as well as a favorable biocompatibility on human keratinocytes. Confocal microscopy images showed ND uptake and internalization by S. pyogenes starting from 3 h of incubation up to 24 h. According to cell counts, NDs displayed long-term antimicrobial efficacy against streptococci significantly counteracting their proliferation up to 24 h, thanks to the known chitosan antimicrobial properties. Intriguingly, Ery-NDs were generally more effective (104–103 log10 CFU/mL), than free-erythromycin (105 log10 CFU/mL), in the direct killing of streptococci, probably due to Ery-NDs adsorption by bacteria and prolonged release kinetics of erythromycin inside S. pyogenes cells. Based on these findings, NDs and proper Ery-NDs appear to be the most promising and skin-friendly approaches for the topical treatment of streptococcal skin infections allowing wound healing during hypoxia.

show abstract

Detection of Streptococcus pyogenes Virulence Factors

Cited by 3 publications

References 26 publications

The Group A Streptococcal Vaccine Candidate VAX-A1 Protects against Group B Streptococcus Infection via Cross-Reactive IgG Targeting Virulence Factor C5a Peptidase

The Group A Streptococcal Vaccine Candidate VAX-A1 Protects against Group B Streptococcus Infection via Cross-Reactive IgG Targeting Virulence Factor C5a Peptidase

Antibiotic resistance in tap water during the summer season – preliminary research

Design, Characterization, and Biological Activities of Erythromycin-Loaded Nanodroplets to Counteract Infected Chronic Wounds Due to Streptococcus pyogenes

Contact Info

Product

Resources

About