1992
DOI: 10.3147/jsfp.27.191
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Detection of Striped Jack Nervous Necrosis Virus(SJNNV) by Enzyme-Linked Immunosorbent Assay(ELISA).

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Cited by 114 publications
(57 citation statements)
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“…Several authors studied the horizontal transmission of this virus during outbreaks and confirmed it by various experimental studies [3,15,88]. Arimoto et al [2] was probably the first to demonstrate vertical transmission by detecting the virus in the fertilized eggs and also in 65 % of the striped jack brood fish by antibody-based ELISA technique. Vertical transmission has also been demonstrated in European seabass [26], Japanese flounder, barfin flounder [123], Atlantic halibut [51] and Asian seabass (L. calcarifer) [7].…”
Section: Epidemiologymentioning
confidence: 88%
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“…Several authors studied the horizontal transmission of this virus during outbreaks and confirmed it by various experimental studies [3,15,88]. Arimoto et al [2] was probably the first to demonstrate vertical transmission by detecting the virus in the fertilized eggs and also in 65 % of the striped jack brood fish by antibody-based ELISA technique. Vertical transmission has also been demonstrated in European seabass [26], Japanese flounder, barfin flounder [123], Atlantic halibut [51] and Asian seabass (L. calcarifer) [7].…”
Section: Epidemiologymentioning
confidence: 88%
“…The test involves the detection of specific nodavirus antibodies in blood and other body fluids. ELISA was one of the first immunological methods to be developed [2,24] to detect SJNNV but was not very sensitive. It is commonly used since a large number and a variety of samples can be screened rapidly by these methods.…”
Section: Immunoassaysmentioning
confidence: 99%
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“…Cohabitation infection of striped jack (Arimoto et al, 1993) and infection of sea bass via infected water (Baudin Laurencin, 1997) has also been demonstrated. Vertical transmission may also be a possibility since the virus has been detected in the ova ries and fertilized eggs of striped jack (Arimoto et al, 1992) and sea bass (Baudin Laurencin, 1997). It has also been detected in striped jack larvae from positive spawners but not from apparently negative ones, and PCR-based segregation of virus-carrying spawners was found to be an effective prophylactic measure in striped jack hatcheries (Mushiake et al, 1994 species.…”
Section: B) Viral Diseasesmentioning
confidence: 99%
“…9,22,27 Systematic monitoring and diagnosis of NNV infections was first initiated in Japan in 1992. 1 Two cell lines, the striped snakehead fish (Channa striatus) cell line (SSN-1) and the spotted grouper (Epinephelus coioides) fin cell line (GF-1), can be used for NNV propagation. 5,15 Several diagnostic tests, such as fluorescent antibody test and enzymelinked immunosorbent assay (ELISA) can be used to differentiate NNV from other fish viruses.…”
Section: Introductionmentioning
confidence: 99%