Detection of structural changes based on Mie-scattering analyses of mouse fibroblast L929 cells before and after necrosis

Baselt, Tobias; Richter, Clemens; Rudek, F.; Nelsen, Bryan; Lasagni, Andrés Fabían; Hartmann, Péter

doi:10.1117/12.2307485

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…1. 15 A spectrally filtered and collimated SC light source, developed and manufactured in cooperation with fiberware GmbH, was used. 16 The SC light source emits 1.1-ns pulses, a repetition rate of 8 kHz, and was filtered to a spectral bandwidth between 450 and 650 nm.…”

Section: Light-scattering Measurementsmentioning

confidence: 99%

Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

et al. 2018

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Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis," J.Abstract. The scattering properties of biological tissue are highly dependent on the structure size, refractive index, and wavelength of the incident light. Furthermore, these scattering characteristics are strongly influenced by movements of the scattering objects. A method is developed to determine the angular-and spectral-resolved scattering properties that enabled the characterization of biological nano-and microscaled cell structures. Nanosecond pulses from a spectrally filtered supercontinuum light source are captured and time-resolved to depress background noise and minimize disruptive effects of the biological cells. The scattering characteristics of a monolayer of mouse fibroblast L929 cells are measured at defined wavelengths in a standard cell culture plate. Because of the size and distribution of the scattering structures, a Fourier transform-based Mie scattering scheme is used to analyze the data. The system is tested to detect structural changes of mouse fibroblast L929 cells before and after poisoning with Triton X100. The final result is the development of a contamination-free method to study pathological changes in cell cultures, necrosis, or other cell-damaging effects.

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Section: Light-scattering Measurementsmentioning

confidence: 99%

Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

et al. 2018

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“…fibroblast necrosis [7]. While these beads have successfully amplified the Mie scattering intensities measured at a fixed angle, it may be possible to identify the type and morphology of tissue without using such beads (or any other labels).…”

Section: Introductionmentioning

confidence: 99%

Label-Free Mie Scattering Identification of Tumor Tissue Using an Angular Photodiode Array

Bills

Yoon

2020

IEEE Sens. Lett.

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Tumors differ from normal tissues in several meaningful ways including cellular size, morphology, and protein expression, which will accordingly change the refractive index and the size/morphology of cells. There are also important differences in tissue organization and unique tissue specific cell densities. Instead of time-consuming and labor-intensive histology involving the use of a benchtop microscope, a plot of Mie scattering intensities at fixed wavelength against scattering angle, which we referred to as "Mie spectrum," is suggested as an alternative to identify tumor from normal tissues. An angular photodiode array is developed to measure this Mie spectrum with three different light emitting diodes (blue, green and red) as light sources. The resulting Mie spectra show characteristic peaks for rat colonic tissues, and substantial differences can be found between tumor vs. normal tissues. Two peaks were identified at 120° and 150° scattering angles, potentially representing capillaries and colon cells, respectively. Contributions from crypts and goblet cells, represented by the scattering at 140°, were minimal. Substantial differences between tumor and normal tissues were found with 45°-70° light irradiation angles. Index Terms-colon cancer; rat model, light scattering, tissue biopsy. I.

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Angular Photodiode Array for the Identification of Colorectal Carcinoma by Mie Scatter

Bills

Yoon

2019

2019 Ieee Sensors

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Detection of structural changes based on Mie-scattering analyses of mouse fibroblast L929 cells before and after necrosis

Cited by 3 publications

References 7 publications

Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

Label-Free Mie Scattering Identification of Tumor Tissue Using an Angular Photodiode Array

Angular Photodiode Array for the Identification of Colorectal Carcinoma by Mie Scatter

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