Detection of Sugars on Paper Chromatograms

Trevelyan, W. E.; Procter, D P; Harrison, Joseph S.

doi:10.1038/166444b0

Cited by 3,820 publications

(950 citation statements)

References 4 publications

Supporting

Mentioning

942

Contrasting

Unclassified

Order By: Relevance

“…1 chromatography paper using ethyl acetate-pyridine-water (8:2:1, v/v) as the solvent system (Rendig et al, 1964) or on MN300 cellulose thin-layer chromatography plates (Macherey-Nagel, Diiren, Germany) using formic acid--methyl ethyl ketone-tert.-butanol--water (15 : 30 : 40 : 15, v/v) (Vomhof & Tucker, 1951) as the solvent system. The sugars were detected with orcinol (Bevenue & Williams, 1951), aniline (McCready & McComb, 1954), or silver nitrate (Trevelyan et al, 1950) reagents.…”

Section: Methodsmentioning

confidence: 99%

Function of the omasum in several ruminant species

Prins

Hungate

Prast

1972

Comparative Biochemistry and Physiology Part A: Physiology

View full text Add to dashboard Cite

Abstract--1. The main function of the omasum is to represent an arrangement for continuance of the fermentation in all species.2. The pH of omasal contents of sheep and cattle is near neutral and the rate of fermentation equals the rate in the rumen.3. Omasal contents of deer have a lower pH and show a lower fementation rate when compared with the rumen and small amounts of soluble carbohydrate accumulate, contributing not more than 0"3 g of glucose to the animal per day.

show abstract

Section: Methodsmentioning

confidence: 99%

Function of the omasum in several ruminant species

Prins

Hungate

Prast

1972

Comparative Biochemistry and Physiology Part A: Physiology

View full text Add to dashboard Cite

show abstract

“…1 paper irrigated with propanol:ethyl acetate:water (14:2:7) or ethyl acetate:pyridine:water (10:4:3). Sugar spots were visualized with AgNOa/NaOH [25].…”

Section: Methodsmentioning

confidence: 99%

A glycogen‐debranching enzyme from Cytophaga

et al. 1970

View full text Add to dashboard Cite

“…and ethyl acetate-pyridine-water (2:1:2, by vol.). Reducing sugars were detected by spraying chromatograms with aniline hydrogen phthalate or with alkaline AgNO3 (Trevelyan, Procter & Harrison, 1950). Amino sugars were detected by means of ninhydrin and by acetylacetone-p-dimethylaminobenzaldehyde (Partridge, 1948).…”

Section: Biosynthesis Of Intestinal Mucinsmentioning

confidence: 99%