Detection of sul1 and sul2 genes in sulfonamide-resistant bacteria (SRB) from sewage, aquaculture sources, animal wastes and hospital wastewater in South-West Nigeria

Adekanmbi, Abimbola Olumide; Adejoba, Anuoluwapo Tosin; Banjo, Omowunmi Abosede; Saki, Morteza

doi:10.1016/j.genrep.2020.100742

Cited by 23 publications

(13 citation statements)

References 26 publications

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“…The interpretation criteria (sensitive/resistant) for the antibiotics were determined on the basis of the zone diameters provided in CLSI 2017 [ 17 ]. An isolate was designated multiple antibiotic resistant if it was resistant to at least three antibiotics classes [ [18] , [19] , [20] , [21] ]. The antibiotics used in this study were selected on the basis of their clinical and agricultural significance.…”

Section: Methodsmentioning

confidence: 99%

Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa

Nzima

Adegoke

Ofon

et al. 2020

New Microbes and New Infections

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The spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in different environments. This study aimed to evaluate the patterns of antibiotic resistance and ESBL genes among E. coli isolates collected from wastewater and recipient surface water in South Africa. Fifteen samples containing nine wastewater and six river water samples were collected from a local wastewater treatment plant. The E. coli isolates were detected using standard microbiology methods. Antibiotic susceptibility testing was performed using disc diffusion agar. The occurrence of bla CTX-M , bla SHV and bla TEM ESBL genes was investigated by PCR. Exactly 140 isolates were selected from the primary enumeration plates with a log 10 CFU/mL count that ranged from 4.1 to 4.2 (influent), 4.2 to 4.5 (biofilter) and 2.5 to 3.3 (effluent). The wastewater effluent showed an impact on the receiving water environment, as the treatment efficiency was 92% and the downstream log 10 CFU/mL count (range, 3.6–3.8 log 10 CFU/mL) was higher than the upstream count (range, 3.3–3.6 log 10 CFU/mL). Antibiotic testing results showed that 40% to 100% of E. coli isolates were resistant to ampicillin, penicillin, tetracycline and cefotaxime but susceptible to imipenem, meropenem and ciprofloxacin. A total of 40 studied isolates (28.6%) had both the bla TEM and bla CTX-M genes, while no bla SHV was detected. The wastewater treatment plants contributed multidrug-resistant ESBL-producing E. coli isolates that can be potential environmental health risks. Regular monitoring policies are recommended to prevent the spread of antibiotic resistance in the region.

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Section: Methodsmentioning

confidence: 99%

Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa

Nzima

Adegoke

Ofon

et al. 2020

New Microbes and New Infections

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show abstract

“…14 About 96% of bacteria isolated in this study were resistant to ampicillin which is not in line with a study conducted in Iran and Nigeria. 15,16 The high proportion of ampicillinresistant bacteria detected in the current study could be due to the wide and misuse of antibiotics in the hospital environment. Greater than 26% of bacteria isolated in the current study were resistant to ceftriaxone.…”

Section: Discussionmentioning

confidence: 80%

Distribution and antimicrobial resistance profile of bacteria recovered from sewage system of health institutions found in Hawassa, Sidama Regional State, Ethiopia: A descriptive study

2021

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Objectives: The aim of the study was to determine the distribution and antimicrobial susceptibility profile of bacteria recovered from the sewage systems of health institutions found in Hawassa, Sidama Regional State, Ethiopia. Methods: A cross-sectional study was conducted from 20 October 2020 to 1 December 2020. A total of 27 sewage samples were collected at two points, namely, before entering the septic tank and from the septic tank of seven health institutions. Samples were inoculated onto Mannitol salt agar, Blood agar, and MacConkey agar, and incubated for 24 h at 37°C. Bacteria were identified using colony morphology, Gram staining, and biochemical tests. An antimicrobial susceptibility test was performed using the Kirby–Bauer disk diffusion method. Data were analyzed by SPSS, version 25, and results were presented in text and tables. Results: All sewage samples ( n = 27) examined in the current study contained potential pathogenic bacteria. Overall, 129 different types of bacteria were identified. Of isolated bacteria, 14 (10.8%) were Gram positive, while 115 (89.2%) were Gram negative. The most prevalent bacteria were Escherichia coli ( n = 27, 20.9%) followed by Shigella species ( n = 26, 20.2%), Pseudomonas species ( n = 25, 19.4%), Salmonella species ( n = 25, 19.4%), Staphylococcus aureus ( n = 14, 10.9%), and Klebsiella species ( n = 12, 9.3%). All bacteria were susceptible to azithromycin. About 80% of bacteria were resistant to ampicillin, whereas greater than 80% of bacteria were susceptible to norfloxacin, ciprofloxacin, and gentamicin. Conclusion: All sewage systems of health institutions included in the current study contained different types of pathogenic bacteria, which are resistant to commonly prescribed antibiotics.

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“…Out of the total AMR recorded in manure samples, ampicillin and sulfadiazine resistance contributed to 14% and 13%, respectively. Sulfonamides are widely present in human and animal wastes (Adekanmbi et al 2020 ; Huygens et al 2021 ). A recent study reported that 75% of sulfonamide-resistant bacteria were found to be resistant to ampicillin (Adekanmbi et al 2020 ), which is in accordance with the data obtained in the present study.…”

Section: Resultsmentioning

confidence: 99%

“…Sulfonamides are widely present in human and animal wastes (Adekanmbi et al 2020 ; Huygens et al 2021 ). A recent study reported that 75% of sulfonamide-resistant bacteria were found to be resistant to ampicillin (Adekanmbi et al 2020 ), which is in accordance with the data obtained in the present study. Pearson correlation ( r ) was drawn among profiles of antibiotic resistance bacteria (ARB), physico-chemical parameters, and bacterial abundance in the manure samples (Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Nutrient concentrations affect the antimicrobial resistance profiles of cattle manures

Shrivas

Choudhary

Hariprasad

et al. 2021

Environ Sci Pollut Res

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Detection of sul1 and sul2 genes in sulfonamide-resistant bacteria (SRB) from sewage, aquaculture sources, animal wastes and hospital wastewater in South-West Nigeria

Cited by 23 publications

References 26 publications

Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa

Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa

Distribution and antimicrobial resistance profile of bacteria recovered from sewage system of health institutions found in Hawassa, Sidama Regional State, Ethiopia: A descriptive study

Nutrient concentrations affect the antimicrobial resistance profiles of cattle manures

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