Detection of T-cell receptor γ gene rearrangement in paraffin-embedded T or natural killer/T-cell lymphoma samples using the BIOMED-2 protocol

Miyata‐Takata, Tomoko; Takata, Katsuyoshi; Yamanouchi, Sachiko; Sato, Yasuharu; Harada, Mai; Oka, Takashi; Tanaka, Takehiro; Maeda, Yoshinobu; Tanimoto, Mitsune; Yoshino, Tadashi

doi:10.3109/10428194.2013.871634

Cited by 20 publications

(14 citation statements)

References 9 publications

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“…The results were positive in 71% of the AITL. [15] The overall prognosis of these tumors is poor with a median survival less than three years in most studies (16). The treatment is mainly based on a conventional chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

Is CD10 Antibody Useful in the Diagnosis of Angioimmunoblastic T-Cell Lymphoma?

Mlika

Helal

Laabidi

et al. 2015

Journal of Immunoassay and Immunochemistry

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Angioimmunoblastic T-cell lymphoma (AITL) is a rare lymphoma accounting for less than 1% of non Hodgkin lymphomas. The lack of specific clinical and histologic features in addition to the multiplicity of morphologic mimickers makes the diagnosis challenging. Some authors reported the utility of CD10 antibody as a diagnostic marker. Our aim is to explore the value of this marker through a presentation of a study about four cases diagnosed over a six-year-period and a mini-review of the literature. We present a study of 4 cases of AITL diagnosed in lymph node biopsies. Microscopic findings showed in all cases early AITL characterized by a pattern I. The diagnosis of AITL was made in all cases based on the morphologic and immunohistochemical features. The CD3 antibody was expressed in all cases but CD10 antibody was negative in all cases. All the patients died and the initiation of a conventional chemotherapy wasn't possible in all cases because of the rapid course of the disease. Because of the small number of our cases, we can't rule out a possible diagnostic value of CD10 but its negativity in all our cases makes us doubt its sensitivity.

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Section: Discussionmentioning

confidence: 99%

Is CD10 Antibody Useful in the Diagnosis of Angioimmunoblastic T-Cell Lymphoma?

Mlika

Helal

Laabidi

et al. 2015

Journal of Immunoassay and Immunochemistry

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“…T-cell receptor g chain (TCRG) VgIf and Vg10 primers (tube A) and TCRG Vg9 and Vg11 primers (tube B) were used for TCR gene rearrangement study, and each of the tubes (A and B) also included 2 primers targeting the J segments (Jg1.1/2.1 and Jg1.3/Jg2.3) as previously reported. 12 11 The products of PCR reactions were subsequently analyzed by ABI PRISM 310 Genetic Analyzer and Gene Mapper software version 3.7 (Applied Biosystems, CA).…”

Section: Polymerase Chain Reaction For Immunoglobulin Heavy Chain Andmentioning

confidence: 99%

Epstein-Barr Virus–infected Cells in IgG4-related Lymphadenopathy With Comparison With Extranodal IgG4-related Disease

Takeuchi¹,

Sato

Ozawa

et al. 2014

American Journal of Surgical Pathology

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IgG4-related lymphadenopathy with increased numbers of Epstein-Barr virus (EBV)-infected cells has been reported but not fully described. We analyzed 31 cases of IgG4-related lymphadenopathy and 24 cases of extranodal IgG4-related diseases for their possible relationship with EBV. Other types of reactive lymph nodes (22) and angioimmunoblastic T-cell lymphoma (AITL) (10) were also studied for comparison. EBV-encoded RNA (EBER) in situ hybridization revealed EBER(+) cells in 18 of 31 cases (58%) of IgG4-related lymphadenopathy. Increased EBER(+) cells were found in only 4 of 22 (18.1%) non-IgG4-related reactive lymphoid hyperplasia in patients of a similar age (P=0.002) and in only 5 of 24 (21%) extranodal IgG4-related biopsies (P=0.006). Interestingly, all patients with EBER(+) progressively transformed germinal center-type IgG4-related lymphadenopathy had systemic lymphadenopathy and/or extranodal involvement. AITL also is associated with EBV, and IgG4-related lymphadenopathy sometimes mimics the morphology of AITL; however, the number of IgG4(+) cells in AITL was significantly less than that in IgG4-related lymphadenopathy (P<0.001). Increased numbers of regulatory T cells are seen in IgG4-related disease; however, there was not a significant difference between the EBER(+) and EBER(-) cases. In conclusion, the presence of increased numbers of EBV-infected cells in IgG4-related lymphadenopathy, compared with other reactive lymphadenopathy or extranodal IgG4-related disease, suggests that there may be a relationship at least between nodal IgG4-related disease and EBV. It is important to avoid overdiagnosing these cases as malignant lymphomas or EBV-related lymphoproliferative disorders.

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“…A convention used in the laboratory at Colorado State University is to call a result positive (clonal) if the clonal peak is three times the height of the baseline, in accordance with recently published human studies (Miyata-Takata et al, 2014). A convention used in the laboratory at Colorado State University is to call a result positive (clonal) if the clonal peak is three times the height of the baseline, in accordance with recently published human studies (Miyata-Takata et al, 2014).…”

Section: Discussionmentioning

confidence: 92%