Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography

Abstract

Abstract. Wutsqa YU, Suratman, Sari SA. 2021. Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography. Biofarmasi J Nat Prod Biochem 19: 65-69. The diversity of ferns on this earth is very high, and the potential and benefits for human life are ornamental plants, food materials, and medicines. The benefits of not all ferns are known because of the lack of information on these ferns, one of the ferns is Lindsaea obtusa J. Sm. Ex Hook. The phytochemical test is the first step in research, searching for new active compounds derived from natural ingredients. This study was conducted to determine the differences of terpenoid and steroid compounds profile in L. obtusa extract using Thin Layer Chromatography (TLC). Extraction of L. obtusa using maceration techniques with methanol and n-hexane solvents. The extraction results will be used for phytochemical screening using the TLC technique with GF254 silica gel plate as a stationary phase, and a mobile phase is chloroform: n-hexane (9: 1 v/v), n-hexane: ethyl acetate (4: 1 v/v), and methanol: ethyl acetate (5: 1 v/v). Detection of terpenoid and steroids using Lieberman Burchard reagent. The result shows that L. obtusa extract contains terpenoid and steroid compounds. Quantitatively, the secondary metabolites of L. obtusa extract were soluble in methanol solvent more than the soluble compounds in n-hexane solvent. However, qualitatively, steroid compounds that dissolve in n-hexane solvents have a more varied Rf value than terpenoids, especially in the mobile phase of chloroform: n-hexane (9: 1 v/v) and n-hexane: ethyl acetate (4: 1 v/v). L. obtusa contains more various steroid compounds than terpenoid compounds.

Phytochemical Analysis by FTIR of Zanthoxylum Acanthopodium, DC Fruit Ethanol Extract, N-hexan, Ethyl Acetate and Water Fraction

“…( 13 ) The steroids and triterpens identification was Liebermen-Bourchar test by adding chloroform, acetic anhydride, and H 2 SO 4 to the test tube. The formation of dark pink or red color or reddish brown in the lower layer indicated the presence of steroids and green color in the upper layer for triterpens ( 13 , 14 ).…”

Phytochemical Screening, Thin Layer Chromatography and Fourier Transform Infra-Red Spectroscopy Analysis of Eleutherine Bulbous (Mill.) Urb Bulb Extract

“…12 The Liebermen-Bourchar test were used to identify the steroids and triterpene and after chloroform, acetic anhydride, and H 2 SO 4 the extract would showed a dark pink or red color or reddish brown in the lower layer if it contained steroids and green color in the upper layer for triterpene. 12,13 Thin Layer Chromatography (TLC)…”

The Bioactivity Potential of Acmella Paniculata Plant Extract in Antioxidant Activity by Two Different Extraction Methods

“…Steroids are part of fat groups therefore it tends to dissolve in non-polar solvents. Hence, steroids prefer to be extracted in polar and semi-polar solvents [22,23]. Steroids are present by using this method because of DES have multiple interactions such as hydrogen bonding, dipole-dipole, dipoleinduced dipole, Van der Waals (dispersion) and ion-dipole interactions that allow extraction of polar and non-polar components [24].…”

“…Steroids are present by using this method because of DES have multiple interactions such as hydrogen bonding, dipole-dipole, dipoleinduced dipole, Van der Waals (dispersion) and ion-dipole interactions that allow extraction of polar and non-polar components [24]. Next, terpenoid compounds also can be attracted to semipolar or even polar solvents as it possesses a hydroxyl group attached to the hydrocarbon chain [23]. The results found that both steroids and terpenoids are present in leaf extract of A. paniculata but not at an extremely high concentration.…”