Detection of the antimicrobial resistance genes blaTEM-1, cfxA, tetQ, tetM, tetW and ermC in endodontic infections of a Mexican population

Domínguez-Pérez, Rúben Abraham; Torre-Luna, Rocio De la; Ahumada-Cantillano, Mariana; Vázquez-Garcidueñas, Ma. Soledad; Pérez-Serrano, Rosa M.; Martínez-Martínez, Rita Elizabeth; Guillén-Nepita, Ana Laura

doi:10.1016/j.jgar.2018.05.011

Cited by 10 publications

(3 citation statements)

References 34 publications

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“…The screening of ARGs in HCW samples discovered the prevalence of bla TEM-1 in FC and FV samples. Bla TEM is a well known beta-lactam resistance determinant that has been widely reported in clinical isolates of gram-negative bacteria ( Domínguez-Pérez et al, 2018 ; Haider et al, 2022 ; Katiyar et al, 2020 ). The bla TEM-1 gene encodes TEM-1 class A beta-lactamase that confers resistance to beta-lactam antibiotics such as penicillin and first-generation cephalosporins by hydrolyzing the beta-lactam ring of antibiotics ( Salverda et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pre-treated healthcare wastes during COVID-19 pandemic reveals pathogenic microbiota, antibiotics residues, and antibiotic resistance genes against beta-lactams

Siew

Musa

Sabri

et al. 2023

Environmental Research

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Section: Discussionmentioning

confidence: 99%

Evaluation of pre-treated healthcare wastes during COVID-19 pandemic reveals pathogenic microbiota, antibiotics residues, and antibiotic resistance genes against beta-lactams

Siew

Musa

Sabri

et al. 2023

Environmental Research

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“…The oral microbiota has been described as a reservoir for several antibiotic resistance genes [23]. Like other multispecies biofilms, oral bacterial species are arranged in proximity, which frequently leads to interactions such as quorum-sensing systems, food chains, and exchange of virulence and resistance genes [24], including endodontic biofilms involved in pulp and periapical pathologies [25].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Decreases the Efficacy of Endodontic Filling Pastes for Root Canal Treatment in Children′s Teeth

et al. 2021

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The antibacterial efficacy of antimicrobial filling pastes (AFP) used in the root canal treatment of primary teeth has been widely reported. However, antibiotic resistance as an emerging global problem could impact their current efficacy. This study aimed to evaluate the efficacy of two common AFP on susceptible or resistant bacteria isolated from primary necrotic molars. Microbiological samples were obtained and cultured from the root canals of 34 children. In total, 96 colony-forming units were obtained to determine their resistance to tetracycline, rifampicin, and chloramphenicol. They were identified as S. mutans or E. faecalis using polymerase chain reaction. The antimicrobial activity of CTZ paste (chloramphenicol, tetracycline, zinc oxide, and eugenol) and Guedes-Pinto modified (GPM) paste (rifampicin, prednisolone, iodoform, and camphorated paramonochlorophenol) were tested against the identified and selected microorganisms. Larger size inhibition zones were observed in both species when the tested strains were susceptible to the antibiotics in the AFP preparation. The efficacy of AFP containing antibiotics depends on the antibiotic resistance profile of the strain. Antibiotic resistance and its effect on the AFP were shown, which calls into question the use of simplified endodontic techniques that depend on antibiotics, since in these cases these techniques could not clinically eliminate resistant bacteria from the root canal.

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“…Tet (32) was first detected in Clostridium-related human colonic anaerobes, which also carried tet(W) [61]. Tet(Q) is also previously mostly detected in the oral microbiome of humans [62,63]. In recent study, tet(Q) and CfxA were detected in the microbiota of dogs and their owners, and the conclusion that the oral microbiota of dogs and pet owners share the same AMR genes was made [64].…”

Section: Zoonotic Potential Of the Amr Genesmentioning

confidence: 95%

Comparison of Oral Microbial Composition and Determinants Encoding Antimicrobial Resistance in Dogs and Their Owners

Šakarnytė,

Šiugždinienė,

Žymantienė

et al. 2023

Antibiotics

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Consolidated studies on animal, human, and environmental health have become very important for understanding emerging zoonotic diseases and the spread of antimicrobial resistance (AMR). The aim of this study was to analyse the oral microbiomes of healthy dogs and their owners, including determinants of AMR. Shotgun metagenomic sequencing detected 299 bacterial species in pets and their owners, from which 70 species were carried by dogs and 229 species by humans. Results demonstrated a unique microbial composition of dogs and their owners. At an order level, Bacteroidales were the most prevalent oral microbiota of dogs with significantly lower prevalence in their owners where Actinomycetales and Lactobacillales predominated. Porphyromonas and Corynebacterium were the most prevalent genera in dogs, whereas Streptococcus and Actinomyces were in animal owners. The resistances to macrolides, tetracyclines, lincosamides and Cfx family A class broad-spectrum β-lactamase were detected in both animal and human microbiomes. Resistance determinants to amphenicols, aminoglycosides, sulphonamides, and quaternary ammonium compounds were detected exceptionally in dogs. In conclusion, the study demonstrated different bacterial composition in oral microbiomes of healthy dogs without clinical signs of periodontal disease and their owners. Due to the low numbers of the samples tested, further investigations with an increased number of samples should be performed.

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Detection of the antimicrobial resistance genes blaTEM-1, cfxA, tetQ, tetM, tetW and ermC in endodontic infections of a Mexican population

Cited by 10 publications

References 34 publications

Evaluation of pre-treated healthcare wastes during COVID-19 pandemic reveals pathogenic microbiota, antibiotics residues, and antibiotic resistance genes against beta-lactams

Evaluation of pre-treated healthcare wastes during COVID-19 pandemic reveals pathogenic microbiota, antibiotics residues, and antibiotic resistance genes against beta-lactams

Antibiotic Resistance Decreases the Efficacy of Endodontic Filling Pastes for Root Canal Treatment in Children′s Teeth

Comparison of Oral Microbial Composition and Determinants Encoding Antimicrobial Resistance in Dogs and Their Owners

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