Detection of the genome of <i>Chlamydophila abortus</i> in samples taken from the uteri of 304 sheep at an abattoir

Michalopolou, E.; Leigh, A. J.; Córdoba, Leonel

doi:10.1136/vr.161.5.153

Cited by 7 publications

(8 citation statements)

References 13 publications

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“…Chlamydophila abortus was the most prevalent pathogen in this study, confirming the importance of this abortigenic microorganism in south‐east Spain, as in other countries of Europe and North America (Moeller 2001, Chanton‐Greutmann and others 2002, Szeredi and others 2006, Michalopoulou and others 2007). Histopathological analysis of the placental samples showed that the positive results for C abortus in the sheep were mainly associated with the presence of severe necrotising placentitis (lesion grade 2), although some cases that were positive for C abortus by IHC and/or PCR techniques showed only mild signs of placentitis (lesion grade 1).…”

Section: Discussionsupporting

confidence: 84%

Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin‐embedded samples

Navarro¹,

Ortega²,

Buendía³

et al. 2009

Veterinary Record

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A histological study was carried out on 58 formalin-fixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. Sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for PCR detection of Chlamydophila abortus, Coxiella burnetii, Salmonella Abortusovis, Brucella melitensis, Listeria monocytogenes and Toxoplasma gondii. Most of the cotyledons showed different degrees of placentitis. The proportional agreement between the two techniques was 0.879 (kappa value 0.746). C abortus was the most prevalent pathogen. Mixed infections were common.

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Section: Discussionsupporting

confidence: 84%

Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin‐embedded samples

Navarro¹,

Ortega²,

Buendía³

et al. 2009

Veterinary Record

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“…21 Previously, a PCR assay was used for the detection of C. abortus in samples of uterine tissue collected from pregnant sheep in an abattoir and resulted in a prevalence of 31%, with a significantly higher prevalence in pregnant animals (47%). 25 In the current study, the duplex real-time PCR assay detected C. abortus in 55% of the studied farms, suggesting that the bacterium may be more widely disseminated in sheep flocks than previously documented.…”

Section: Discussionmentioning

confidence: 44%

“…Regarding the diagnosis of toxoplasmosis, 10 out of the 13 (77%) cases diagnosed as toxoplasmosis using standard methods were also found to be positive by the PCR (Table 6). Nine out of the 10 cases (17)(18)(19)(20)(21)(22)(23)(24)(25)(26) found to be positive based on histopathological findings were also diagnosed as toxoplasmosis by the duplex real-time PCR. Only 1 out of the 3 cases (14-16) diagnosed as toxoplasmosis by the LAT method was positive by PCR.…”

Section: Diagnosis Of Farm Abortions: Real-time Pcr Versus Standard Methodsmentioning

confidence: 99%

Application of quantitative real-time polymerase chain reaction for the diagnosis of toxoplasmosis and enzootic abortion of ewes

et al. 2012

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Toxoplasma gondii and Chlamydophila abortus are the 2 most common infectious causes of ovine abortion worldwide. These obligate intracellular pathogens are associated with severe placentitis leading to abortion or stillbirth in pregnant ewes, and resulting in significant economic losses. The objectives of the current study were the development, validation, and application of a duplex real-time polymerase chain reaction (PCR) assay capable of quantifying the burden of infection by T. gondii and C. abortus in material submitted for diagnostic purposes. The validation was carried out using samples from ewes experimentally infected with these organisms. Based on the numbers of genome copies detected, an arbitrary cutoff level was established to correlate with significant pathological changes sufficient to give rise to abortion. When the PCR assay was applied to samples from 66 Irish farms with naturally occurring outbreaks of ovine abortion, toxoplasmosis and enzootic abortion of ewes (EAE) accounted for 14% and 20% of the farms, respectively, while on 6% of the farms, there was evidence of dual infection. When standard diagnostic techniques including histopathological examination, serological analysis, chlamydial antigen detection, and bacteriological culture, were used on samples from the same farms, toxoplasmosis was diagnosed in 17% of farms, and EAE in 12%; dual infection was diagnosed on 3% of the farms. In general, good agreement was found between the PCR and the standard methods. The duplex real-time PCR assay developed in this study has proved to be a very sensitive and rapid tool that might provide a valuable addition to the methods currently available for routine diagnosis of ovine abortions.

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“…There are some variables that may reduce the amount of bacteria to undetectable numbers, which makes PCR-based detection and bacteria isolation more difficult. For example, isolation can be achieved at a higher level of success when animals are sampled at the beginning or at the end of pregnancy than when sampling is carried out at midgestation (Michalopoulou et al 2007). It has also been shown that the chlamydiae are normally shed more abundantly in estrus than during the diestrus period (Livingstone et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico

Campos-Hernández

Vázquez-Chagoyán

Salem

et al. 2014

Trop Anim Health Prod

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The aim of this study was to determine the seroprevalence and presence of Chlamydia abortus in Saanen breed female goats from commercial dairy goat farms under intensive production in the municipality of Guanajuato, Mexico. Sera were collected to determine the prevalence of anti-C. abortus IgG antibodies using recombinant enzyme-linked immunosorbent assay (rELISA) and cell culture. Polymerase chain reaction (PCR) was used to prove the presence of the pathogen in swab samples collected from the vagina and rectum of selected animals. Additionally, foetal tissue samples from a sudden abortion were collected. C. abortus prevalence in female goats of commercial milking farms sampled in Guanajuato, Mexico, was 4.87% (n = 246). Seropositive animals were found in six out of nine (66.6%) dairy goat farms sampled, and prevalence among animals in individual farms ranged between 3.44 and 13.51%. C. abortus was detected using PCR in spleen tissue from the aborted foetus. PCR-based detection, as well as isolation from vaginal and rectal swabs, was not possible in the present study. Isolation through cell culture was also unsuccessful from aborted foetal tissue samples. In conclusion, the results from rELISA and PCR show that C. abortus is present in dairy goat farms in the state of Guanajuato, Mexico.

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Detection of the genome of Chlamydophila abortus in samples taken from the uteri of 304 sheep at an abattoir

Cited by 7 publications

References 13 publications

Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin‐embedded samples

Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin‐embedded samples

Application of quantitative real-time polymerase chain reaction for the diagnosis of toxoplasmosis and enzootic abortion of ewes

Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico

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