Detection of the hepcidin prepropeptide and mature peptide in liver of rainbow trout

Synthetic hepcidin from fish: Uptake and protection against Vibrio anguillarum in sea bass (Dicentrarchus labrax)

“…The peptide changes to an alpha-helix conformation when in the reduced state, while it shows a β-sheet structure when in the oxidized state through the formation of M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT disulfide bonds [14]. Furthermore, we have shown the presence of this peptide in trout liver and anterior kidney, and that it is upregulated in response to Escherichia coli or Aeromonas salmonicida lipopolysaccharide [15,16]. These results suggest that it participates in cellular defense responses of teleost fish against pathogen-associated molecular patterns (PAMPs), possibly being involved in post-invasion by the infectious agents [17].…”

“…For the detection of Hep1-Rho in Sea bass tissue, imprint sections of anterior intestine, spleen, liver and anterior kidney were fixed on glass slides with methanol for 2 min as described previously [15]. …”

Antimicrobial Peptides from Fish

“…Similar to other AMP genes, fish hepcidins can be induced by exposure to both Gram-positive and Gram-negative bacteria [12,19,25,26,27,34,36,37,42,48,53,56,61,63,72,73,161,162,163,164,165,166]. Moreover, fungi like Saccharomyces cerevisiae [36,61], and tumor cell lines like L-1210 and SAF-1 have shown to induce hepcidin expression as well [36].…”

Molecular cloning and mRNA expression of a hepcidin gene from the spinyhead croaker, Collichthys lucidus

“…The mRNA expression pattern of a gene is closely related to its function. In this study, we examined the expression of CLhepc in eight tissues, showing that expression was highest in the liver, which is seen as a common pattern in most HEPCs (Sang et al, 2006;Fu et al, 2007;Robertson et al, 2009;Oliveira Filho et al., 2010; Nam et al, 2011;Wang et al, 2012;Alvarez et al, 2013). In this sense, CL-hepc may prove to be an important component participating in innate immune activities.…”

“…The sequences of HEPCs have been cloned in many fish species, including both freshwater fish, such as zebrafish (Shike et al, 2004), mud loach (Nam et al, 2011), and rice field eel (Li et al, 2011), and marine fish, including Epinephelus coioides (Qu et al, 2013), Pseudosciaena crocea (Wang et al, 2009), Cynoglossus semilaevis (Wang et al, 2012), rainbow trout (Alvarez et al, 2013), and Chrysophrys major (Chen et al, 2005). HEPC in fish has a similar structure and mRNA distribution to other species, such as mammals, although there are some differences.…”