“…For example, this approach was used in a PCR-RFLP analysis for identification of K-ras gene mutations (Mora et al, 1998). Alternatively, the digestion reaction can be spiked with a DNA fragment that contains the relevant restriction enzyme recognition site and produces fragments of other sizes than those of the amplicon (Lima-Neto et al, 2009). Plasmides have been used as such internal digestion control and are suitable for this as they contain recognition sites for many of the commonly used restriction enzymes.…”