2009
DOI: 10.1089/gtmb.2008.0147
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Detection of theTLR41196C>T Polymorphism by Mismatched-Polymerase Chain Reaction Using Plasmid DNA as Internal Control in Restriction Fragment Length Polymorphism Assays

Abstract: Blood samples were collected from 102 individuals with acute myocardial infarction (AMI) and 108 non-AMI individuals (controls) for DNA extraction and laboratory analyses. The 1196C > T polymorphism in the toll-like receptor 4 (TLR4) gene was amplified by mismatched-polymerase chain reaction (PCR). Amplicons and pBluescript II SK- plasmid were simultaneously digested with endonuclease HincII. Fragments were separated on 2% agarose gels. Plasmid was completely digested using up to 55.2 nmL/L DNA solutions and 1… Show more

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Cited by 6 publications
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“…For example, this approach was used in a PCR-RFLP analysis for identification of K-ras gene mutations (Mora et al, 1998). Alternatively, the digestion reaction can be spiked with a DNA fragment that contains the relevant restriction enzyme recognition site and produces fragments of other sizes than those of the amplicon (Lima-Neto et al, 2009). Plasmides have been used as such internal digestion control and are suitable for this as they contain recognition sites for many of the commonly used restriction enzymes.…”
mentioning
confidence: 99%
“…For example, this approach was used in a PCR-RFLP analysis for identification of K-ras gene mutations (Mora et al, 1998). Alternatively, the digestion reaction can be spiked with a DNA fragment that contains the relevant restriction enzyme recognition site and produces fragments of other sizes than those of the amplicon (Lima-Neto et al, 2009). Plasmides have been used as such internal digestion control and are suitable for this as they contain recognition sites for many of the commonly used restriction enzymes.…”
mentioning
confidence: 99%