Detection of the mcr-1 gene in Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains isolated from broilers

Lopes, Hugo Peralva; Costa, Gisllany Alves; Pinto, Ana C.L.Q.; Machado, Leandro S.; Cunha, Nathalie Costa da; Nascimento, Elmiro Rosendo do; Pereira, Vla; Abreu, Dayse L.C.

doi:10.1590/1678-5150-pvb-5983

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…Of the 213 isolated cloacal samples and broiler carcasses strains of E. coli, 57 (26.76%) were characterized as atypical Enteropathogenic E. coli (EPEC) and 35 (16.43%) as Shigatoxigenic E. coli (STEC). The mcr-1 gene was found in 9 of 213, and 3.5% (2/57) becomes of the EPEC strains, and 5.7% (2/35) becomes of the STEC strains [72].…”

Section: Brazilmentioning

confidence: 98%

mcr-mediated colistin resistance in South America, a One Health approach: a review

Daza-Cardona

Buenhombre

Fontenelle

et al. 2021

Reviews in Medical Microbiology

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Antimicrobial resistance is recognized as one of the most important global human health problems in the 21st century. The balance between clinical need and resistance prevention is further compromised by agricultural antibiotic use as some countries have actively used colistin in animal production. Antimicrobial-resistant microorganisms have been found in humans, food, animals, plants, and the environment (water, soil, and air), and they can move between ecosystems. The problem of antimicrobial resistance is currently seen in the concept: One Health. This approach encourages interdisciplinary collaborative efforts that work locally. The beginnings of the mcr gene's presence in America dates to 2012 and the first report of disseminating the mcr-1 gene was described for Kelp gulls (Larus dominicanus) from Ushuaia, Argentina. It can be associated with the possible dissemination of the gene in the American continent through migratory birds. The mcr-1 gene is disseminated in countries, such as Argentina,

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Section: Brazilmentioning

confidence: 98%

mcr-mediated colistin resistance in South America, a One Health approach: a review

Daza-Cardona

Buenhombre

Fontenelle

et al. 2021

Reviews in Medical Microbiology

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“…Although resistance to tigecycline has been reported in 29.3% of E. coli strains isolated from organic chicken farms (Musa et al, 2021), the authors did not rule out the possibility of an environmental origin for the resistant strains. In contrast, resistance to colistin has been detected in several investigations in the poultry production chain, including in Brazil (Lopes et al, 2020;Lentz et al, 2021), with frequencies ranging from 1.8% (Perrin-Guyomard et al, 2016;Pesciaroli et al, 2020) to as high as 57.94% (Barbieri et al, 2021) and 79.8% (Irrgang et al, 2016). In addition, food animals have been suggested as possible reservoirs and spreaders of genes that confer resistance to this class of antimicrobials (Irrgang et al, 2016;Perrin-Guyomard et al, 2016;Guenther et al, 2017;Monte et al, 2017;Yassin et al, 2017;Bitrus et al, 2018;Zhang et al, 2018).…”

Section: Tablementioning

confidence: 99%

Antimicrobial resistance profiles in Escherichia coli isolated from whole-chicken carcasses from conventional, antibiotic-free, and organic rearing systems

Vieira

Oliveira

Cibulski

et al. 2022

Semina: Ciênc. Agrár.

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Antimicrobial resistance (AMR) is a growing concern in human and animal health. Public discussions on these issues have contributed to an increased demand for antibiotic-free food. Studies comparing the antimicrobial resistance profiles of bacteria in foodstuffs originating from farming systems with restrictions on the use of antimicrobials are scarce. This study aimed to assess the antimicrobial resistance profiles of generic Escherichia coli isolated from whole chickens originating from farming systems with and without restrictions on the use of antimicrobials. For this purpose, three groups of E. coli strains were formed: (GC) from chickens reared in conventional production systems, without restriction on the use of antimicrobials (n=72); (GL) from chickens reared in farming systems certified as free of any antibiotic use (n=72); and (GO) from chickens from an organic farming system (n=72). Whole chicken units were individually rinsed as recommended by ISO 17604:2015, and E. coli was isolated from the rinse suspension. To evaluate the resistance profile, E. coli strains were tested against 12 antimicrobials using broth microdilution or disk diffusion tests. Eighty strains (40.7%) were found to be fully susceptible to the tested antimicrobials, and 23.6% were multidrug resistant. The highest frequencies of resistance were observed to tetracycline (GC=37,5%; GL=34,7%; GO=25%) and trimethoprim (GC=27,8%; GL=34,7%; GO=22,2%). In the case of multidrug resistant strains, GC presented 32% (n=23) of strains with multidrug resistance characteristics whereas the GL and GO groups presented 22% (n=16) and 17% (n=12), respectively. As for the totally susceptible strains, a frequency of 56% of Tsus strains was observed in the organic group, whereas this frequency was 33% in the GC and GL groups. Using GC as a reference, the Poisson regression model showed a higher occurrence of fully susceptible E. coli strains, as well as lower frequencies of multidrug resistance and resistance to ampicillin and nalidixic acid in GO. The GL group exhibited the lowest frequency of ampicillin resistance. These observations suggest that the lower selection pressure for antimicrobial use in the farming system may be reflected in the resistance profile of bacteria present in foodstuffs purchased by consumers.

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“…The prevalence of the mcr‐1 gene in Enterobacteriaceae is unknown in several countries (Arcilla et al, 2016). In Brazil, recent studies have provided epidemiological data that contribute to understanding mcr‐1 mediated resistance in E. coli isolated from humans (Rossi et al, 2016), animals, food and environmental sources (Fernandes et al, 2016; Lopes, Costa, et al, 2020). In free‐living pigeons, this is the first Brazilian report of the occurrence of an mcr‐1 gene.…”

Section: Discussionmentioning

confidence: 99%

Multidrug‐resistant Escherichia coli from free‐living pigeons (Columba livia): Insights into antibiotic environmental contamination and detection of resistance genes

Bueno

Loiko

Vidaletti

et al. 2022

Zoonoses and Public Health

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Bacterial resistance is a public and one health problem. Free‐living birds can be reservoirs of multidrug‐resistant bacteria and resistance genes. This study aimed to characterize the antimicrobial resistance of Escherichia coli isolated from free‐living urban pigeons (Columba livia) in South Brazil. Ninety‐two animals were sampled, and one isolate was obtained from each one. The isolates were characterized, and the antimicrobial resistance profile and beta‐lactam and colistin resistance genes were investigated. The isolates were classified as phylogroups B1 (35%), B2 (33%), A (16%) and D (16%), and 14% of the strains had the eae virulence gene. All isolates were resistant to at least one antimicrobial, and 63% of them were multidrug‐resistant. Geographical location where the pigeons were captured and presence of the eae gene were associated with multidrug resistance. blaVIM and mcr‐1 genes were detected in one and two isolates, respectively. This is the first report of these genes in E. coli of pigeons. The blaVIM‐positive isolate was classified as Shiga toxin‐producing E. coli, and the isolates with mcr‐1 were classified as Enterohaemorrhagic E. coli and Enteropathogenic E. coli, which raise additional concerns related to public health since these are zoonotic pathotypes. The results reveal that pigeons carry multidrug‐resistant pathogenic E. coli, which may interest public health. Nonetheless, further studies on whether these animals are sources of contamination for humans must be performed to understand their role in spreading antimicrobial resistance.

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Detection of the mcr-1 gene in Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains isolated from broilers

Cited by 3 publications

References 28 publications

mcr-mediated colistin resistance in South America, a One Health approach: a review

mcr-mediated colistin resistance in South America, a One Health approach: a review

Antimicrobial resistance profiles in Escherichia coli isolated from whole-chicken carcasses from conventional, antibiotic-free, and organic rearing systems

Multidrug‐resistant Escherichia coli from free‐living pigeons (Columba livia): Insights into antibiotic environmental contamination and detection of resistance genes

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