Detection of the Philadelphia chromosome in interphase nuclei

Arnoldus, E. P. J.; Wiegant, J.; Noordermeer, I.A.; Wessels, J.; Beverstock, Geoffrey C.; Grosveld, Gerard; Ploeg, M. van der; Raap, A.K.

doi:10.1159/000132972

Cited by 174 publications

(65 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All details concerning pre-treatment of slides, clonogenic cells produced and the frequencies of either early or late CAFC displayed a similar distribution over the various hybridization, washing and immunochemical detection were as reported. 32 The probes used were: (1) Cos-ABL-18, a 40-kb fragment containing the 3′ coding region of the human abl gene; and (2) Cos-bcr-51 containing a 35.5-kb fragment rep- Table 1 Mean representing the coincidence of a red and green signal. In contained fewer signals were not scored as Ph + or Ph − , but bution.…”

Section: Enrichment Of Cd34-positive Progenitor Cells Stromal Feedersmentioning

confidence: 99%

Efficient long-term maintenance of chronic myeloid leukemic cobblestone area forming cells on a murine stromal cell line

et al. 1997

View full text Add to dashboard Cite

represent spleen colony-forming stem cells (CFU-S day-12), cobblestone area whereas the cells giving rise to late appearing, more permanent CAs are related to primitive stem cells with long-term in vivo repopulating ability. 21,[24][25][26] Similarly, in the human CAFC assay both the phenotypes of the CAFC subsets 23 and the in vivo repopulation data in SCID mice 27,28 and men 29 are conIntroduction cordant with the assumption that CAFC wk 2-3 represent more transiently repopulating stem cells, whereas CAFC wk Chronic myeloid leukemia (CML) is a malignant disorder aris-5-8 are indicators of stem cells that induce stable chimerism ing from a pluripotent hematopoietic stem cell. 1 The disease in vivo. We have previously shown that the FBMD-1 stromal is characterized by excessive production of mature blood cells cell line allows long-term growth of AML leukemic stem belonging predominantly to the myeloid lineage. Malignant cells. 30 In the present study we have investigated the possicells are characterized cytogenetically by a translocation bility of propagating CML stem cells over an extended period between chromosomes 9 and 22 (t(9;22)) and, at the molecuof time in order to improve the analysis of their frequency and lar level, by the bcr/abl hybrid gene resulting from the transpoability to generate committed progenitors. sition of the c-abl proto-oncogene on chromosome 9 to the breakpoint cluster region (bcr) on chromosome 22. sent from CML patients in first chronic phase.

show abstract

Section: Enrichment Of Cd34-positive Progenitor Cells Stromal Feedersmentioning

confidence: 99%

Efficient long-term maintenance of chronic myeloid leukemic cobblestone area forming cells on a murine stromal cell line

et al. 1997

View full text Add to dashboard Cite

show abstract

“…In certain diagnostic applications, such as the detection of trisomy 21, small probes are more informative than painting of whole chromosomes, because the more focal signals are easier to quantitate [48,160]. Furthermore, Single probes flanking or spanning a chromosomal breakpoint can be used to detect a diagnostically important breaking event [161][162][163] or to characterize further a breakpoint region [11,164,165]. An example of a breakpoint in the short arm of chromosome 11 from a Potter's facies Syndrome patient was previously reported and is shown in Figure IB.…”

Section: Clinical Applicationsmentioning

confidence: 99%

“…An example of a breakpoint in the short arm of chromosome 11 from a Potter's facies Syndrome patient was previously reported and is shown in Figure IB. This approach seems particularly useful in analyzing specific translocations in blood cell tumors [162,163,165]. The details, implications, and prospects of this application of nonisotopic in situ hybridization are reviewed elsewhere in this Journal [3].…”

Section: Clinical Applicationsmentioning

confidence: 99%

Analysis of genes and chromosomes by nonisotopic in situ hybridization

Lichter

Boyle

Cremer

et al. 1991

Genetic Analysis: Biomolecular Engineering

224

102

View full text Add to dashboard Cite

“…FISH studies of interphase and metaphase cells using digoxygenin-labelled 5Ј BCR (BCRH1), and biotin-labelled 3Ј ABL (cosABL8) cosmid probes showed signal patterns consistent with this finding. Using methods previously described, 6 about one-third of cells (95/274 interphase; 26/72 metaphase) showed four distinct and separate fluorescent signals, two red and two green, indicating that those cells lacked the BCR-ABL hybrid gene (Figure 2a). Karyotype studies in September 1995 showed an increase in the proportion of Ph-negative cells to 84% (16/19), whereas FISH studies showed approximately equal numbers of BCR-ABL-positive (89) and -negative (93) interphase cells.…”

Section: Cytogenetic and Fish Analysesmentioning

confidence: 98%

True

1999

Leukemia

View full text Add to dashboard Cite

We report a patient with Philadelphia (Ph)-positive, BCR-ABL rearrangement positive, chronic myeloid leukemia (CML) with a prolonged chronic phase of 24 years who was first prescribed alpha-2 interferon 22 years after initial diagnosis. This therapy was tolerated poorly on account of thrombocytopenia, but an eventual major cytogenetic response was followed soon afterwards by transformation to terminal acute myeloid leukemia (AML). Cytogenetic studies indicated that the transformed myeloblasts were karyotypically normal and Ph negative. Although polymerase chain reaction (PCR) analysis of total leukemic mRNA remained BCR-ABL positive, other molecular studies, including Southern blotting and fluorescent in situ hybridization (FISH) analyses, showed that myeloblasts were BCR-ABL rearrangement negative. PCR-based clonality studies using an X-chromosome-linked restriction fragment polymorphism within the phosphoglycerate kinase gene (PGK 1 ) further showed that the Ph-negative blast cells had a different clonal origin from the Ph-positive clone of chronic phase. We suggest that cases of underlying Ph-negative leukemic transformation in Ph-positive CML warrant further study and should be considered for trial of intensive remission induction therapy as appropriate for acute leukemia.

show abstract

Detection of the Philadelphia chromosome in interphase nuclei

Cited by 174 publications

References 2 publications

Efficient long-term maintenance of chronic myeloid leukemic cobblestone area forming cells on a murine stromal cell line

Efficient long-term maintenance of chronic myeloid leukemic cobblestone area forming cells on a murine stromal cell line

Analysis of genes and chromosomes by nonisotopic in situ hybridization

True

Contact Info

Product

Resources

About