Detection of the ryanodine receptor mutation associated with malignant hyperthermia in purebred swine populations1

Houde, Alain; Pommier, Serge A.; Roy, Raymond C.

doi:10.2527/1993.7161414x

Cited by 52 publications

(23 citation statements)

References 9 publications

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“…Ao contrário da caracterização pela exposição ao anestésico Halotano, a técnica molecular permite a clara identificação dos animais heterozigotos (HOUDE et al, 1993;REMPEL et al, 1993;WEBB, 1996). A extração de DNA genômico de folículo piloso mostrou-se um método rápido, eficiente e simples, que torna a caracterização molecular do gene hal aplicável a qualquer animal dentro do sistema de produção (BAUEROVA et al, 1995;BASTOS et al, 2000).…”

Section: Resultsunclassified

Efeito do gene do estresse suíno sobre características de quantidade e qualidade de carcaça

Bastos¹,

Federizzi²,

Deschamps³

et al. 2001

R. Bras. Zootec.

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RESUMO -O gene do estresse suíno (gene hal), em homozigose recessiva (nn), está associado com a ocorrência da Síndrome do Estresse Porcino (PSS) e com a ocorrência da carne pálida, mole e exudativa (PSE). Em heterozigose (Nn), está relacionado com diminuição na qualidade da carne porém, com maior peso de carcaça. Neste estudo, foi caracterizado o genótipo do gene hal de 160 suínos por meio de análise do DNA extraído de um único folículo piloso. Estes animais foram abatidos e, após, mensuradas características de carcaça de cada um. Entre os 160 animais analisados, 82 (52,58%) foram caracterizados como NN, 67 (41,80%) como Nn e nove (5,62%) como nn. A variação das características de carcaça entre os genótipos foi avaliada por análise de variância, usando o SAS. Os animais dos três genótipos não diferiram quanto ao peso da carcaça quente, à espessura de toucinho, profundidade de músculo, porcentagem de carne magra e cor do músculo longissimus dorsi. Entretanto, a variação da cor ao longo da carcaça foi menor em animais NN (40,82%) do que em Nn (49,77%) e nn (53,83%). Estes resultados indicam que a presença do gene hal tanto em heterozigose como em homozigose recessiva não está associada ao maior peso de carcaça e a utilização destes animais pode acarretar em perda na qualidade da carne. Devido a isto, o uso intencional de animais homozigotos recessivos ou heterozigotos em programas de cruzamento deve ser desestimulado. Palavras-chave: carcaça suína, gene halotano, PCR, PSE, PSS Effect of Swine Stress Gene on Quality and Quantity of CarcassABSTRACT -The swine stress gene (hal gene) in recessive homozygosis (nn) is associated with the occurrence of the Porcine Stress Syndrome (PSS) and with the occurrence of pale, soft and exudative pork (PSE). In heterozygosis (Nn) it is related to low quality of carcass, but higher carcass weight. This study has characterized the genotype of hal gene by DNA-testing, obtained by alkaline extraction from a single hair root, in a sample of 160 swines. These animals were slaughtered and carcass traits for each animal were measured. Among the 160 animals, 82 (52.58%) were identified as NN, 67 (41.80%) as Nn, and 9 (5.62%) as nn. Variation in carcass traits across genotypes was evaluated by analysis of variance, using SAS. The NN, Nn and nn animals did not differ for: hot carcass weight, backfat thickness, muscle depth, lean meat percentage, and longissimus dorsi muscle color. However, variation in carcass color was lower for NN (40.82%) than for Nn (49.77%) and nn (53.83%) animals. These results indicate that the presence of hal gene in heterozygosis or recessive homozigosis was not associated with better carcass weight and the utilization of these animals may lead to lower carcass quality. Therefore, the intentional use of heterozygous and recessive homozygous animals should not be encouraged.

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Section: Resultsunclassified

Efeito do gene do estresse suíno sobre características de quantidade e qualidade de carcaça

Bastos¹,

Federizzi²,

Deschamps³

et al. 2001

R. Bras. Zootec.

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“…The microtubes were then placed in the 96-sample tray of the thermocycler (MJ-Research PTC-100). The amplification program, modified from Fujii et al (1991) and Houde et al (1993), consisted of initial denaturation at 94°C for 3 min and 35 cycles at 94°C for 45 s, 68°C for 1 min and 72°C for 1 min, with a final polymerization step at 72°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Relationship between the Porcine Stress Syndrome gene and pork quality traits of F2 pigs resulting from divergent crosses

et al. 2005

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The PSS genotypes of 596 F 2 pigs produced by initial mating of Brazilian commercial sows and native boars were characterized by PCR-RFLP and the pork quality traits were evaluated. Among the 596 pigs studied, 493 (82.7%) were NN and 103 (17.3%) were Nn. There were no differences between NN and Nn pigs in the following pork qualities: pH u (5.71 ± 0.16 vs 5.70 ± 0.11), intramuscular fat (1.55 ± 0.64% vs 1.65 ± 0.67%), shear force (5552 ± 878 g/1.2 cm vs 5507 ± 826 g/1.2 cm), lightness (44.96 ± 2.05 vs 45.01 ± 1.92), redness (0.64 ± 0.60 vs 0.79 ± 0.55), yellowness (6.62 ± 0.56 vs 6.65 ± 0.48), hue (84.28 ± 5.53 vs 83.41 ± 4.85), or chroma (6.68 ± 0.52 vs 6.73 ± 0.52). However, pork from Nn pigs had a significantly (p < 0.05) lower pH 45 (6.41 ± 0.27 vs 6.51 ± 0.26) and greater drip (3.92 ± 1.90% vs 3.06 ± 1.60%), cooking (33.29 ± 2.26% vs 32.50 ± 2.54%) and total (35.67 ± 2.48% vs 34.01 ± 2.58%) loss compared to that of NN pigs. These results indicate that, even in divergent crosses, PSS gene carriers produce pork of poorer quality.

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“…The microtubes were then added to a 96-sample tray of a MJResearch PTC-100 thermocycler. The amplification program, modified from Fujii et al (1991) and Houde et al (1993), consisted of initial denaturation at 94°C for 3 min and 35 cycles at 94°C/45 s, 68°C/1 min and 72°C/1 min, and a final polymerization step at 72°C for 5 min.…”

Section: Introductionmentioning

confidence: 99%

Relationship between the Porcine Stress Syndrome gene and carcass and performance traits in F2 pigs resulting from divergent crosses

et al. 2005

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The PSS genotypes of 596 F 2 pigs produced by initial mating of Brazilian native boars commercial sows and were characterized by PCR-RFLP and their carcass and performance traits were evaluated. Among the 596 animals analyzed, 493 (82.72%) were characterized as NN and 103 (17.28%) as Nn. With respect to carcass traits, Nn animals presented higher (p < 0.05) right half carcass weight, left half carcass weight, loin depth and loin eye area, and lower shoulder backfat thickness, backfat thickness between last and next to last but one lumbar vertebrae and backfat thickness after last rib at 6.5 cm from the midline compared to NN animals. Nn animals also showed (p < 0.05) higher values for most of the cut yields, indicating higher cutting yields for animals carrying the n allele and lower values for bacon depth, confirming lower fat deposition in carcass. In addition, Nn animals presented (p < 0.05) lower values for the performance trait weight at 105 days of age. These results indicate that animals carrying the PSS gene generate leaner carcasses, higher cut yields, and that the effects of the gene can be observed even in divergent crosses.

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Detection of the ryanodine receptor mutation associated with malignant hyperthermia in purebred swine populations1

Cited by 52 publications

References 9 publications

Efeito do gene do estresse suíno sobre características de quantidade e qualidade de carcaça

Efeito do gene do estresse suíno sobre características de quantidade e qualidade de carcaça

Relationship between the Porcine Stress Syndrome gene and pork quality traits of F2 pigs resulting from divergent crosses

Relationship between the Porcine Stress Syndrome gene and carcass and performance traits in F2 pigs resulting from divergent crosses

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