Detection of the shrimp pathogenic bacteria, Vibrio harveyi, by a quartz crystal microbalance-specific antibody based sensor

Buchatip, Suthawan; Ananthanawat, Cheeraporn; Sithigorngul, Paisarn; Sangvanich, Polkit; Rengpipat, Sirirat; Hoven, Voravee P.

doi:10.1016/j.snb.2009.12.003

Cited by 38 publications

(23 citation statements)

References 22 publications

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“…In addition, the analyte binding and immobilization level were decreased, possibly due to steric hindrance. Furthermore, high concentrations of the antibody could also promote nonspecific adsorption and increase the cost of the test [19]. Hence, the polyclonal antibody concentration of 70 µg·mL −1 was chosen for further sensor studies.…”

Section: Immobilization Of the Antibody On Sammentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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Abstract:Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 10 6 CFU·mL −1 . The sensitivity of the assay was further enhanced to 4 × 10 4 CFU·mL −1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (10 6 -10 7 CFU·mL −1 ). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.

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Section: Immobilization Of the Antibody On Sammentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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“…Because SAs are biological macromolecules, their native structures should be carefully preserved during immobilization to maintain their functionality . Researchers have used SAM techniques to successfully fabricate various QCM biosensors, which exhibited excellent response and bioactivity . Herein, SAM was selected as the immobilization method to anchor proteins.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22] Researchers have used SAM techniques to successfully fabricate various QCM biosensors, which exhibited excellent response and bioactivity. [23][24][25][26] Herein, SAM was selected as the immobilization method to anchor proteins.…”

Section: Introductionmentioning

confidence: 99%

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

Chen

Zhang

et al. 2012

Chirality

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Quartz crystal microbalance (QCM) biosensor was used for the chiral recognition of five pairs of enantiomers by using goat serum albumin (GSA) and rabbit serum albumin (RbSA) as chiral selectors. Serum albumin (SA) was immobilized on the QCM through the self-assembled monolayer technique, and the surface concentration of GSA and RbSA were 8.8 × 10(-12) mol cm(-2) and 1.2 × 10(-11) mol cm(-2) , respectively. The QCM biosensors showed excellent sensitivity and selectivity. Meanwhile, the chiral recognition of SA sensors was quite species dependent. There were differences between GSA and RbSA sensors in the ability and the preference of chiral recognition. To R,S-1,2,3,4-tetrahydro-1-naphthylamine (R,S-1-TNA), R,S-1-(4-methoxyphenyl)ethylamine (R,S-4-MPEA), and R,S-1-(3-methoxyphenyl)ethylamine (R,S-3-MPEA), the preference of the stereoselective SA-drug binding of the two kinds of SA sensors were consistent. However, to R,S-2-octanol (R, S-2-OT) and R,S-methyl lactate (R,S-MEL), the two kinds of SA sensors had opposite chiral recognition preference. Moreover, the interactions of SA and the five pairs of enantiomers have been further investigated through ultraviolet (UV) and fluorescent (FL) spectra. The UV/FL results were in accordance with the consequence of QCM.

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“…The detection of bacteria by QCM was achieved in several papers using either antibody binding (immunosensor) or novel methods. The pathogenic bacteria Vibrio harveyi , which causes morbidity in commercial shrimp cultures, was detected using an antibody that showed no detectable cross reactivity to Vibrio vulnificus nor Vibrio parahaemolyticus (Buchatip et al ., ). Live Escherichia coli were detected using antibodies immobilised on a long chain organosilicon self assembled monolayer, with the nature of the monolayer being important for successful detection (Dinh et al ., ).…”

Section: Bacteriamentioning

confidence: 97%

A Survey of the 2010 Quartz Crystal Microbalance Literature

Speight

Cooper

2012

J of Molecular Recognition

135

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In 2010 there has again been an increase in the number of papers published involving piezoelectric acoustic sensors, or quartz crystal microbalances (QCM), when compared to the last period reviewed 2006-2009. The average number of QCM publications per annum was 124 in the period 2001-2005, 223 in the period 2006-9, and 273 in 2010. There are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase), and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterisation of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights theoretical and practical aspects of the principals that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells, and membrane interfaces.

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Detection of the shrimp pathogenic bacteria, Vibrio harveyi, by a quartz crystal microbalance-specific antibody based sensor

Cited by 38 publications

References 22 publications

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

A Survey of the 2010 Quartz Crystal Microbalance Literature

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