Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

Mascarenhas, Joana D’Arc Pereira; Bezerra, Delana Andreza Melo; Silva, René R.; Silva, Mayara J. M.; Júnior, Edivaldo Costa Sousa; Soares, Luana da Silva

doi:10.1007/s00705-016-2882-z

Cited by 10 publications

(15 citation statements)

References 27 publications

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“…Regarding the circulation of RVA in canines and birds, the prevalence was 53% and 29%, respectively. Although in the Amazon region there are records of RVA, RVD, RVF and RVG that infect birds [57][58] and RVD in migratory birds [59], all were detected by RT-PCR assays differently from the present study which detected the RVA by RT-qPCR involving a variety of animal species.…”

Section: Discussioncontrasting

confidence: 93%

Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon

et al. 2018

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Acute gastroenteritis is one of the main causes of mortality in humans and young animals. Domestic and mainly wild animals such as bats, small rodents and birds are highly diversified animals in relation to their habitats and ecological niches and are widely distributed geographically in environments of forest fragmentation in some areas of the Amazon, being considered important sources for viruses that affect humans and other animals. Due to the anthropical activities, these animals changed their natural habitat and adapted to urbanized environments, thus representing risks to human and animal health. Although the knowledge of the global diversity of enteric viruses is scarce, there are reports demonstrating the detection of rotavirus in domestic animals and animals of productive systems, such as bovines and pigs. The present study investigated the prevalence of Rotavirus A in 648 fecal samples of different animal species from the northeastern mesoregion of the state of Pará, Brazil, which is characterized as an urbanized area with forest fragments. The fecal specimens were collected from October 2014 to April 2016 and subjected to a Qualitative Real-Time Polymerase Chain Reaction (RT-qPCR), using the NSP3 gene as a target. It was observed that 27.5% (178/648) of the samples presented positive results for RVA, with 178 samples distributed in birds (23.6%), canines (21.35%), chiropterans (17.98%), bovines (14.6%), horses (8.43%), small rodents (6.74%), pigs (3.93%) and felines (3.37%), demonstrating the circulation of RVA in domestic animals and suggesting that such proximity could cause transmissions between different species and the occurrence of rearrangements in the genome of RVA as already described in the literature, associated to the traces of environmental degradation in the studied areas.

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Section: Discussioncontrasting

confidence: 93%

Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon

et al. 2018

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“…Next-generation sequencing of jejunal segments from 3-and 7-day-old chicks revealed multiple viruses in RSS-affected and unaffected chicks including avian nephritis virus, avian rotavirus, and gallivirus. Avian nephritis virus and avian rotavirus were previously reported in Brazil 8,9,23,24,28 and in other countries. 7,10,19,30,34,38,43,44 Similar to our findings, some researchers found avian nephritis virus in chicks at 3 and 7 days of age, but avian rotavirus was only detected from chicks in the second week of age, 8 diverging from our study that found avian rotavirus in chicks at 7 days of age.…”

Section: Discussionmentioning

confidence: 89%

Runting and Stunting Syndrome in Broiler Chickens: Histopathology and Association With a Novel Picornavirus

et al. 2020

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Runting stunting syndrome (RSS) in commercial chickens has been reported worldwide, and although several studies have attempted to clarify the cause and describe the lesions, there are gaps in knowledge of the epidemiology, pathogenesis, and etiology. The study objective was to use commercial chicks naturally affected by RSS to describe the histologic changes of RSS in all segments of the small intestine in chicks of different ages and to identify viral gene sequences in affected chicks and their association with histologic lesions. Chicks lacking clinical signs but from the same houses and from unaffected houses were used as controls. The average weight of affected chicks was significantly lower than expected for their flocks. Macroscopically, the small intestines had paler serosa, with watery, mucoid, or foamy contents and poorly digested food. Histologic lesions were characterized by necrotic crypts, crypt dilation, and flattening of the crypt epithelium. Histomorphometry of the intestines revealed villous atrophy especially in the jejunum and ileum. Histologic changes in other organs were not observed. Random next-generation sequencing of total RNA extracted from formalin-fixed paraffin-embedded tissues detected avian nephritis virus, avian rotavirus, and picornavirus in jejunal segments from 7-day-old chicks. No viruses were detected in the jejunum of 1-day-old chicks. Detection of picornaviral reads was significantly associated ( P < .05) with histologic lesions of RSS. Sequence analysis of the picornavirus revealed genetic similarity with the genus Gallivirus. Using in situ hybridization for galliviral nucleic acid sequences, the signal was associated with crypt lesion severity, although signal was detected both in chicks with and without RSS.

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“…In summary, all fecal samples from neotropical migratory birds were negative for PBV, ARV and AvRVA and only AvRVD was detected in Calidris pusilla as the only pathogen in these neotropical migratory birds. Nevertheless, it is valid to correlate the importance of the finding in the context of the Brazilian poultry industry, that is configured as an important segment of agribusiness exploring the probable risks of migratory birds as enteric virus reservoir for commercial poultry or vice versa due to previous detections of AvRVD (Avian Rotavirus D), AvRVA (Avian Rotavirus A), AvRVF (Avian Rotavirus F) and AvRVG (Avian Rotavirus G) in broilers in Brazilian territory (Bezerra et al, 2012;Luz et al, 2014;Silva et al, 2013;Mascarenhas et al, 2016).…”

Section: Investigation Of Enteric Viruses In the Feces Of Neotropicalmentioning

confidence: 99%

Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil

Guerreiro

Moraes

Marinho

et al. 2018

Rev. Bras. Cienc. Avic.

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Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities.

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Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

Cited by 10 publications

References 27 publications

Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon

Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon

Runting and Stunting Syndrome in Broiler Chickens: Histopathology and Association With a Novel Picornavirus

Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil

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