Detection of the X chromosomes in a Klinefelter boar using a whole human X chromosome painting probe

Mäkinen, Antti; Andersson, Maria; Nikunen, Sanna

doi:10.1016/s0378-4320(98)00136-5

Cited by 19 publications

(10 citation statements)

References 12 publications

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“…To date, only a few studies have been reported on the results of using FISH to distinguish translocations from inversions of small chromosome segments and to accurately identify the break points in such defects [Konfortova et al, 1995;Mäkinen et al, 1998;Pinton et al, 2000Pinton et al, , 2003Ducos et al, 2002]. In our study, dual-color chromosome painting was successful in identifying chromosomes 10 and 13 and to confirm that the exchange was between chromosomes 10 and 13.…”

Section: Discussionmentioning

confidence: 54%

A Cytogenetic Study of Breeding Boars in Canada

Quach

Villagómez

Coppola

et al. 2009

Cytogenet Genome Res

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Chromosome abnormalities are well known for their negative impact on the reproductive performance of carriers. Such abnormalities could have severe effect on animal industries which rely heavily on efficient reproduction. We conducted a cytogenetic survey of breeder pigs from 4 different Canadian farms to investigate the frequency of chromosome abnormalities and to assess their reproductive impact on pig populations. Our study revealed that 50% of the ‘hypoprolific’ boars and 2.5% of the young boars raised for service in artificial insemination were carriers of chromosome anomalies while no chromosome defect was noted in any of the ‘proven’ breeder boars. G-banding technique to determine the type of abnormalities detected 3 previously unreported translocations involving chromosomes 1 and 6, chromosomes 10 and 13 and chromosomes 9 and 14. The reciprocal nature of these translocations was confirmed either using fluorescent in situ hybridization (FISH) technique or immunostaining for synaptonemal complex delineation and were named rcp(1;6)(p22,q12), rcp(10;13), and rcp(9;14) (p24;q27), respectively. Prolificacy of 1/6 and 10/13 translocation carriers was noted to be reduced by more than 40% compared to their normal counterparts while it was reduced by 26% in carriers of the 9/14 translocation. Carriers of 1/6 and 9/14 translocations displayed a higher repeat breeding tendency, compared to their herd average (5 and 16%, respectively). While for the 9/14 translocation the prevalence of stillbirths was lower than that in their herd [8.7 vs. 10.4% (p < 0.001)]. The present results, albeit based on a relatively small number of pigs, indicate that the prevalence of chromosome abnormalities could be much higher in Canadian pigs compared to that reported in European pigs and underline the urgent need to initiate cytogenetic screening programs as one of the effective ways to reduce reproductive problems in Canadian pig populations.

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Section: Discussionmentioning

confidence: 54%

A Cytogenetic Study of Breeding Boars in Canada

Quach

Villagómez

Coppola

et al. 2009

Cytogenet Genome Res

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“…The use of molecular cytogenetic techniques to characterize chromosomal rearrangements, classical in humans, is still little developed in animal species. To our knowledge, only 10 cases have been published so far: 4 in the pig species [17,21,24,25], 3 in cattle [11,16,23], 2 in equines [2,3], and 1 in chicken [28].…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic and molecular characterization of�eight new reciprocal translocations in the pig species. Estimation of their incidence in French populations

et al. 2002

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-Eight new cases of reciprocal translocation in the domestic pig are described. All the rearrangements were highlighted using GTG banding techniques. Chromosome painting experiments were also carried out to confirm the proposed hypotheses and to accurately locate the breakpoints. Three translocations, rcp(4;6)(q21;p14), rcp(2;6)(p17;q27) and rcp(5;17)(p12;q13) were found in boars siring small litters (8.3 and 7.4 piglets born alive per litter, on average, for translocations 2/6 and 5/17, respectively). The remaining five, rcp(5;8)(p12;q21), rcp(15;17)(q24;q21), rcp(7;8)(q24;p21), rcp(5;8)(p11;p23) and rcp(3;15)(q27;q13) were identified in young boars controlled before entering reproduction. A decrease in prolificacy of 22% was estimated for the 3/15 translocation after reproduction of the boar carrier. A parental origin by inheritance of the translocation was established for the (5;8)(p11;p23) translocation.

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“…Laser microdissection, often used to isolate specific cells from fixed sectioned material, is also effective for the isolation of large individual mammalian chromosomes for chromosome paints (Makinen et al 1998;Stich et al 2003). The position ablative laser microbeam (PALM) system uses a UV-laser to cut a sample and an additional laser pulse to catapult the sample into a microfuge tube cap (Schutze and Lahr 1998;Schneider-Stock et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal localization of a proinsulin transgene in Japanese quail by laser pressure catapulting

2006

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Transgenic avian bioreactors produce therapeutic recombinant proteins in egg white. To date, however, methods for transgenic modification of the avian genome or determining transgenic status of individual birds are scarce. The dual, but interrelated, goals of this research were to: (1) develop a method of detecting stable DNA insertion into Japanese quail; and (2) provide a method for gene location on avian chromosomes. We created Teflon-coated coverslip slides to facilitate laser pressure catapulting of avian chromosomes for DNA amplification and nucleotide sequencing. Transgenic G2 Japanese quail, containing germline incorporation of proinsulin, were identified by isolation of chromosomes using laser microdissection and laser pressure catapulting. Subsequent amplification of each chromosome identified 2-5 chromosomes with the proinsulin transgene inserted. Nucleotide sequencing of each chromosomal insertion was identical to the proinsulin portion of the original vector. By applying laser pressure catapulting and PCR of individual chromosomes, we were able to determine that the transgene correctly inserted into avian chromosomes and that the majority of the insertions occurred within microchromosomes. Because many potential therapeutic transgenes have similar or nearly identical nucleotide sequence to the host's native gene, laser microdissection and subsequent analysis may be required for detailed documentation of transgene expression before proceeding with transgenic protein production.

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Detection of the X chromosomes in a Klinefelter boar using a whole human X chromosome painting probe

Cited by 19 publications

References 12 publications

A Cytogenetic Study of Breeding Boars in Canada

A Cytogenetic Study of Breeding Boars in Canada

Cytogenetic and molecular characterization of�eight new reciprocal translocations in the pig species. Estimation of their incidence in French populations

Chromosomal localization of a proinsulin transgene in Japanese quail by laser pressure catapulting

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