Detection of Theileria equi and Babesia caballi infections in Venezuelan horses using Competitive-Inhibition ELISA and PCR

Rosales, Romel; Rangel-Rivas, Ariadna; Escalona, América; Jordan, Luis Segundo; Gonzatti, Mary Isabel; Aso, Pedro María; Perrone, Trina; Silva-Iturriza, Adriana; Mijares, Alfrédo

doi:10.1016/j.vetpar.2013.02.004

Cited by 38 publications

(24 citation statements)

References 25 publications

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“…They found that the molecular technique was more sensitive. Different results were found by Rosales et al (2013) in diagnosing B. caballi in Venezuelan horses by means of qPCR and ELISA. They did not manage to detect the hemoparasites by the molecular technique while ELISA identified infection prevalence of 23.2%.…”

Section: Discussionmentioning

confidence: 84%

“…Bilhassi et al (2014) used the qPCR technique to study the level of infection by B. bovis in cattle of different genetic groups and found that blood samples from taurine animals had a significantly higher number of copies of the parasite's DNA than samples from zebu and crossbred animals. Recently, several research groups have conducted experiments involving the association of immunological and molecular tests to detect Babesia (Ferreri et al, 2008;Hüe et al, 2013;Ibrahim et al, 2013;Jaffer et al, 2010;Machado et al, 2012;Mousa et al, 2013;Ramos et al, 2011;Rosales et al, 2013;Shebish et al, 2012). These associations have shown excellent efficiency in detecting infected animals, going a long way toward resolving the problems of predicting the occurrence of babesiosis outbreaks.…”

Section: Introductionmentioning

confidence: 99%

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Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Giglioti

Oliveira

Ibelli

et al. 2017

Ticks and Tick-borne Diseases

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With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtained showed the high sensitivity of the techniques, with 100% of the animals being positive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Giglioti

Oliveira

Ibelli

et al. 2017

Ticks and Tick-borne Diseases

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“…Different trends were observed by other authors using different techniques (Table 3). T. equi was the predominant parasite in 82.14% of the studies in respect to B. caballi , but after excluding two studies [60, 81] due to discrepancies between diagnostic methods regarding the predominant haemoparasite.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Theileria equi and Babesia caballi in horses in Spain

2017

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Equine piroplasmoses are enzootic parasitic diseases distributed worldwide with high incidence in tropical and subtropical regions. In Spain, there is insufficient epidemiological data about equine piroplasmoses. The main aim of the present study was therefore to estimate the prevalence of Theileria equi and Babesia caballi in five regions and obtain information about the risk factors. This study was conducted in the central and south-western regions of Spain, using indirect fluorescence antibody testing (IFAT) in 3,100 sera samples from apparently healthy horses of different ages, breeds, coat colours, genders and geographical locations. The overall seroprevalence was 52%, consisting of 44% seropositive for T. equi and 21% for B. caballi. There was a significant association between age (p < 0.0001), breed (p < 0.004), geographical location (p < 0.0001) and the seroprevalence, but neither the coat colour nor the gender was significantly associated with prevalence. In addition, it was proved that most of the geographic areas showed a moderate to high prevalence. The statistical κ value was used to compare the results obtained by the IFAT and the competitive enzyme-linked immunosorbent assay (cELISA) utilised to test some samples (n = 108) and showed a higher concordance for T. equi (κ = 0.68) than for B. caballi (κ = 0.22). Consequently, this revealed the importance of developing an appropriate technique to detect each haemoparasite.

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“…However, given the low sensitivity of parasitological diagnosis, there is need to combine parasitological diagnosis with molecular diagnostics (Abedi et al, 2014;Salib et al, 2013;Baptista et al, 2013;Rosales et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Serological prevalence of Babesia caballi and Theileria equi in camels and donkeys from Karamoja sub-region, North-eastern Uganda

Nakayima¹,

Nanfuka²,

Aleper³

et al. 2017

J. Vet. Med. Anim. Health

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Equine piroplasmosis is a severe disease of horses caused by the intra-erythrocyte protozoan, Theileria equi and Babesia caballi. T. equi and B. caballi infections were assessed in serum from camels and donkeys using competitive-enzyme-linked immunosorbent assay (cELISA) assay. A total 110 animals were studied including 25 donkeys and 85 camels from two districts viz. Moroto and Amudat in Karamoja sub-region, North-eastern Uganda. All the (100%) donkeys tested were positive for Babesia/T. equi while none of the camels had been exposed to the infection. All animals were negative to B. caballi cELISA. Our findings indicated that all donkeys sampled in Karamoja sub-region have been exposed to T. equi and this could be prevalent in equine population in Uganda. No exposure status to B. caballi was reported. This study represents the first report on the status of T. equi and B. caballi infection in Uganda.

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Detection of Theileria equi and Babesia caballi infections in Venezuelan horses using Competitive-Inhibition ELISA and PCR

Cited by 38 publications

References 25 publications

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Seroprevalence of Theileria equi and Babesia caballi in horses in Spain

Serological prevalence of Babesia caballi and Theileria equi in camels and donkeys from Karamoja sub-region, North-eastern Uganda

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