Detection of Thioamides: Determination of Ethionamide with 2,3-Dichloro-1,4-Naphthoquinone

Devani, M. B.; Shishoo, C. J.; Mody, Hema J.; Raja, PM

doi:10.1002/jps.2600630933

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…Published methods for the determination of ETM in bulk drug and tablets are based on some techniques such as titrimetry (with visual [12] or potentiometric [13,14] end point detection), fluorimetry [15] and visible spectrophotometry employing various chromogenic agents such as dichlone [16,17], iron(III) [18], iron(III)p-phenylenediamine [19], sodium nitroprusside [20,21], pyridylazo-resorcinol-vanadium [22], osmic acid [23], alizarin violet 3B and alizarin brilliant violet R [24], N-bromosuccinimide (NBS)-celestine blue (CB) [25], permanganate [26] and iodine [27].…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric determination of ethionamide in pharmaceuticals using Folin–Ciocalteu reagent and iron(III)-ferricyanide as chromogenic agents

Qarah

Basavaiah

Abdulrahman³

2017

Journal of Taibah University for Science

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Two simple and sensitive spectrophotometric methods are described for the determination of ethionamide (ETM) in pure drug and tablets. The first method is based on the reduction of Folin-Ciocalteu (F-C) reagent by ETM in sodium carbonate medium to form a blue coloured complex, which was measured at 760 nm (Molybdenum-tungsten blue method). In the second method (Prussian blue method), iron(III) was reduced to iron(II) by ETM in HCl medium, in which iron(II) was complexed with ferricyanide, and the resulting Prussian blue was also measured at 760 nm. The absorbance measured in each case was related to the ETM concentration. The experimental conditions were carefully studied and optimised. Beer's law was obeyed in concentration ranges of 1-40 g/ml and 0.2-4.0 g/ml with the Molybdenum-tungsten blue method and the Prussian blue method, respectively, with corresponding molar absorptivity values of 5.72 × 10 3 and 3.18 × 10 4 l/(mol•cm). The limits of detection (LOD) and quantification (LOQ) were 0.09 and 0.27 g/ml for the Molybdenum-tungsten blue method and 0.01 and 0.04 g/ml for the Prussian blue method. Within-day and between-day relative standard deviations (%RSD) at three different concentration levels were <3%, and the respective relative errors (%RE) were ≤2%, implying good accuracy and precision of the methods. The proposed methods were successfully applied to the determination of ETM in bulk powder and tablets, and the results demonstrated that the methods were as accurate and precise as the official method.

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Section: Introductionmentioning

confidence: 99%

Spectrophotometric determination of ethionamide in pharmaceuticals using Folin–Ciocalteu reagent and iron(III)-ferricyanide as chromogenic agents

Qarah

Basavaiah

Abdulrahman³

2017

Journal of Taibah University for Science

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Spectrophotometric Method for the Rapid Determination of Microgram Amounts of Ethionamide

Shah¹,

Agrawal²,

Banerjee³

1981

Analytical Letters

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Application of Cerium (IV) as an Oxidimetric Agent for the Determination of Ethionamide in Pharmaceutical Formulations

Basavaiah

Qarah

Abdulrahman³

2016

Journal of Pharmaceutics

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Two simple methods are described for the determination of ethionamide (ETM) in bulk drug and tablets using cerium (IV) sulphate as the oxidimetric agent. In both methods, the sample solution is treated with a measured excess of cerium (IV) solution in H2SO4 medium, and after a fixed standing time, the residual oxidant is determined either by back titration with standard iron (II) solution to a ferroin end point in titrimetry or by reacting with o-dianisidine followed by measurement of the absorbance of the orange-red coloured product at 470 nm in spectrophotometry. In titrimetry, the reaction proceeded with a stoichiometry of 1 : 2 (ETM : Ce (IV)) and the amount of cerium (IV) consumed by ETM was related to the latter's amount, and the method was applicable over 1.0–8.0 mg of drug. In spectrophotometry, Beer's law was obeyed over the concentration range of 0.5–5.0 μg/mL ETM with a molar absorptivity value of 2.66 × 104 L/(mol·cm). The limits of detection (LOD) and quantification (LOQ) calculated according to ICH guidelines were 0.013 and 0.043 μg/mL, respectively. The proposed titrimetric and spectrophotometric methods were found to yield reliable results when applied to bulk drug and tablets analysis, and hence they can be applied in quality control laboratories.

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Detection of Thioamides: Determination of Ethionamide with 2,3-Dichloro-1,4-Naphthoquinone

Cited by 4 publications

References 11 publications

Spectrophotometric determination of ethionamide in pharmaceuticals using Folin–Ciocalteu reagent and iron(III)-ferricyanide as chromogenic agents

Spectrophotometric determination of ethionamide in pharmaceuticals using Folin–Ciocalteu reagent and iron(III)-ferricyanide as chromogenic agents

Spectrophotometric Method for the Rapid Determination of Microgram Amounts of Ethionamide

Application of Cerium (IV) as an Oxidimetric Agent for the Determination of Ethionamide in Pharmaceutical Formulations

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