Detection of three blood donors with multiple myeloma by routine viral individual‐donor nucleic acid testing screening

Babić, Ivana; Maslovic, Margareta; Vuk, Tomislav; Stanić, Hana Safić; Šestan, P. Topić; Kursar, Marin; Bingulac-Popović, Jasna; Jukić, Irena

doi:10.1111/trf.14319

Cited by 2 publications

(5 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monoclonal immunoglobulins are a rare cause of technical error in the NAT analysers at NZBS, and similar problems have recently been reported by Babic [10]. According to New Zealand Cancer Registry data, there were 386 new diagnoses of multiple myeloma in 2015 [11].…”

Section: Discussionmentioning

confidence: 86%

“…Also, the four highest paraprotein levels in the non‐blocking HSC patient cohort that overlapped with the lowest paraprotein level in the blocker group were of the kappa subtype. Additionally, all three donors in the Babic case series were found to have an IgG lambda monoclonal immunoglobulin . When these cases were included in the blocker group the association with lambda light chains was statistically significant (85.7% vs. 36.6%; P = 0·009).…”

Section: Discussionmentioning

confidence: 95%

“…Finally, the sample size of the blocker group is small. Inclusion of the total protein levels from the Babic case series enhances statistical significance , and we may learn more as additional cases come to light.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, in a letter to the editor of Transfusion, Babic and colleagues reported hardware failures caused by protein clot blockages in the same NAT system . These occurred in three individual donor samples from 639 688 whole blood donations over a four year period (frequency 0·0004%).…”

Section: Introductionmentioning

confidence: 99%

“…These occurred in three individual donor samples from 639 688 whole blood donations over a four year period (frequency 0·0004%). All three donors were found to have an IgG lambda paraprotein by serum protein electrophoresis (SPE) and were subsequently diagnosed with multiple myeloma . The levels of the paraproteins were not described though total protein was between 89·9 and 94·5 g/l.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Paraprotein implicated in hardware failures in nucleic acid testing of blood donors

Leitch¹,

Charlewood²

2019

Vox Sanguinis

View full text Add to dashboard Cite

Background and Objectives Analyser blockage due to gel formation by paraproteins leading to invalid results is a rare problem in viral nucleic acid testing (NAT) at New Zealand Blood Service (NZBS) despite many blood samples tested without problems from individuals with known paraproteins. This study aimed to identify common factors in samples causing blockages. Method Retrospective data were gathered on blood samples known to have blocked analysers at NZBS testing sites. Patients with plasma cell dyscrasia undergoing haematopoietic stem cell (HSC) harvest formed the comparator arm. These patients were identified from registry data of individuals undergoing autologous stem cell transplantation at Auckland City Hospital between 2013 and 2017. Results Four individuals were identified as having blocked analysers between 2010 and 2018. A total of 184 HSC transplant patients were identified, with contemporaneous paraprotein levels available for 177 (96%). Patients with intact immunoglobulin subtypes (134, 73%) were further analysed. Of these, 119 patients (65%) also had total protein and globulin levels available. Mean paraprotein (37.5 g/l), total protein (95.3 g/l), globulin levels (51.5 g/l) and proportion of lambda subtype (75%) were higher in the blocker group compared with non‐blocking comparators (4.7 g/l, 66.8 g/l, 27.7 g/l, 36.6% respectively) (P = 0·03, 0·02, 0·007, 0·12). The highest paraprotein and total protein levels from the non‐blocking cohort overlapped with the lowest of the blocker group. Discussion High protein levels (paraprotein >20 g/l, total protein >75 g/l) and a trend towards lambda subtype were associated with NAT analyser blockage. The overlap with non‐blocking donor samples suggests factors in addition to protein quantity that are also important.

show abstract

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations