Detection of tissue abnormalities in normal mucosa surrounding			 colorectal cancer using an endocytoscopy system

Meroni, Emanuele; Gatteschi, Beatrice; Fasoli, Alberto; Munizzi, Francesco; Frascio, F.; Pugliese, V.; Truini, Mauro

doi:10.1055/s-2007-966098

Cited by 15 publications

(12 citation statements)

References 6 publications

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“…Meroni et al . described a case of endocytoscopy to detect tissue abnormalities in normal mucosa surrounding colorectal cancer . The colorectal specimen was imaged by methylene‐blue‐guided endocytoscopy within 1 h after surgical resection.…”

Section: Advanced Colonoscopic Imaging Using Endocytoscopymentioning

confidence: 99%

“…Meroni et al described a case of endocytoscopy to detect tissue abnormalities in normal mucosa surrounding colorectal cancer. 21 The colorectal specimen was imaged by methylene-blue-guided endocytoscopy within 1 h after surgical resection. By using magnification of ×450, focal changes in the colonic pits were detected by the endocytoscopy system 7 cm away from the tumor within macroscopically normal mucosa, suggesting the presence of an aberrant crypt focus.…”

Section: Advanced Colonoscopic Imaging Using Endocytoscopymentioning

confidence: 99%

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Advanced colonoscopic imaging using endocytoscopy

Neumann

Kudo

Kießlich

2014

Digestive Endoscopy

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Optical biopsy techniques were recently introduced to luminal gastrointestinal endoscopy. These include confocal laser endomicroscopy, spectroscopic imaging techniques and endocytoscopy. Optical biopsy techniques allow on demand in vivo histology during ongoing endoscopy, thereby potentially accelerating clinical diagnosis and specific therapy. In the present review, we focus on endocytoscopy as one of the rapidly emerging optical biopsy techniques. We provide technical details of currently available endocytoscopy systems and give tips on their use in clinical practice. We also summarize applications of endocytoscopy for colorectal pathologies.

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Section: Advanced Colonoscopic Imaging Using Endocytoscopymentioning

confidence: 99%

Section: Advanced Colonoscopic Imaging Using Endocytoscopymentioning

confidence: 99%

Advanced colonoscopic imaging using endocytoscopy

Neumann

Kudo

Kießlich

2014

Digestive Endoscopy

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“…Helicobacter pylori -infection 21 Signet ring cell carcinoma 22 Duodenum Coeliac disease 23,24 Colon Polyps [26][27][28][29][30][31] Amoebic colitis 32 Lung Small cell lung cancer 33,34 Others Bladder carcinoma 35 Intraoperative carcinoma diagnosis 36,38 H H. . N Ne eu um ma an nn n e et t a al l. .…”

Section: Stomachmentioning

confidence: 99%

Review article: in vivo imaging by endocytoscopy

Neumann

Fuchs

Vieth

et al. 2011

Alimentary Pharmacology &Amp; Therapeutics

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Aliment Pharmacol Ther 2011; 33: 1183–1193 Summary Background Endocytoscopy (EC) enables in vivo microscopic imaging at 1400‐fold magnification, thereby allowing the analysis of mucosal structures at the cellular level. In contrast to fluorescence imaging with confocal laser endomicroscopy which allows analysis of mucosal structures up to 250 μm in depth, EC is based on the principle of contact light microscopy and only allows visualisation of the very superficial mucosal layer. Aim To systematically review the feasibility and diagnostic yield of EC for in vivo diagnosis of diseases. Methods A systematic search of the literature on diagnostic interventions in the gastrointestinal tract using EC was performed by searches in MEDLINE, Current Contents, PubMed, cross‐references and references from relevant articles using the search terms ‘endocytoscopy’, ‘endocytoscope’, ‘magnification endoscopy’, ‘endocytoscopic imaging’, ‘virtual histology’ and ‘optical biopsy’. Only full manuscripts and case reports published in English were included. Results Overall twenty‐nine relevant reports were identified. EC was feasible to detect oesophageal squamous cell cancer with sensitivity, specificity and accuracy of 95%, 84% and 82%, respectively. Moreover, EC reached excellent sensitivity and specificity for in vivo diagnosis of colon polyps (91% and 100%, respectively). Other diagnostic applications of EC included diagnosis of Barrett’s oesophagus, Helicobacter pylori, coeliac disease and small cell lung cancer. No serious complications of EC have yet been reported. Conclusions Endocytoscopy is a safe and effective new endoscopic imaging technique to obtain in vivo histology and guided biopsies with high diagnostic accuracy. Therefore, endocytoscopy has the potential to facilitate both diagnosis and patient management.

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“…Endomicroscopy will be key to this immuno-imaging interface [23]. Further, an endocytoscope system has been developed with the possibility of super-high magnification of gastrointestinal mucosa, thus allowing in vivo imaging of living cells [24][25][26].…”

Section: Chromoendoscopy and Endocytoscopy: Emerging Techniquesmentioning

confidence: 99%

Endoscopy, morphology, morphometry and molecular markers: predicting cancer risk in colorectal adenoma

Søreide¹,

Nedrebø

Reite

et al. 2009

Expert Review of Molecular Diagnostics

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The evaluation of short- and long-term risk for developing cancer in patients with colorectal adenomas is controversial. Good, reliable predictors of cancer risk in any adenoma are currently lacking and are limited to adenoma size, number and histologic type. In fact, the evaluation of any adenoma or precancer lesion (e.g., hyperplastic polyps, serrated adenoma or aberrant crypt foci) within the colorectum may be assessed by a number of techniques ranging from direct visualization through the endoscope, to microscopic assessment, and to evaluation at the molecular level. Emerging techniques may yield improved methods of adenoma risk-assessment in the near future. For one, newer endoscopy technologies include chromoendoscopy or endocytoscopy, which now render endoscopists able to resolve the surface and subsurface mucosa at cellular resolution in vivo and in real time - thus, bringing the microscope to the patient's bedside. This new era in endoscopic imaging is dubbed 'histoendoscopy'. Further, while traditional views of classifying protruding and sessile lesions include those of Haggitt, the sm-classification, the Japanese and the so-called Vienna classifications to evaluate neoplasia, the development of new molecular techniques may give way to new methods of classifying preneoplasia and precancerous lesions. This review discusses some pros and cons of risk evaluation technologies in the colorectal tract by endoscopy, microscopy, and quantitative and molecular features. The morphometry-based studies performed over the past decades for the quantitative assessment of cellular and nuclear features within adenomas have failed to yield results amenable for clinical translation and are unlikely to improve further and gain widespread use with current technology. Rather, emerging knowledge of pathway-specific markers through the outlining of a molecular classification will likely be the basis for improved detection and diagnosis. The emerging genomic and proteomic technologies allowing for noninvasive tests to detect (asymptomatic) cancer and neoplasia are discussed. Lastly, the importance of recognizing bias and pitfalls and the adherence to guidelines for biomarker research are addressed.

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Detection of tissue abnormalities in normal mucosa surrounding colorectal cancer using an endocytoscopy system

Cited by 15 publications

References 6 publications

Advanced colonoscopic imaging using endocytoscopy

Advanced colonoscopic imaging using endocytoscopy

Review article: in vivo imaging by endocytoscopy

Endoscopy, morphology, morphometry and molecular markers: predicting cancer risk in colorectal adenoma

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