Detection of tissue factor-positive extracellular vesicles by laser scanning confocal microscopy

Hisada, Yohei; Auriemma, Alyson C.; Alexander, Wyeth; Ay, Cihan; Mackman, Nigel

doi:10.1016/j.thromres.2016.12.021

Cited by 14 publications

(13 citation statements)

References 34 publications

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“…Also, TF, a transmembrane receptor of factor VII/VIIa, can be present on MVs (vesicles often referred to as microparticles, MPs in coagulation studies). The elevated activity levels of this protein have been detected in various diseases (such as in acute liver injury, cirrhosis, urinary tract infection, endotoxemia, influenza, cancer, and related thromboembolism [ 65 ]). The platelet origin of TF on platelet MVs and the overall relevance of TF-bearing platelet MVs have been questioned by several authors [ 66 – 68 ].…”

Section: Evs and The Blood Plasmamentioning

confidence: 99%

Molecular interactions at the surface of extracellular vesicles

et al. 2018

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Extracellular vesicles such as exosomes, microvesicles, apoptotic bodies, and large oncosomes have been shown to participate in a wide variety of biological processes and are currently under intense investigation in many different fields of biomedicine. One of the key features of extracellular vesicles is that they have relatively large surface compared to their volume. Some extracellular vesicle surface molecules are shared with those of the plasma membrane of the releasing cell, while other molecules are characteristic for extracellular vesicular surfaces. Besides proteins, lipids, glycans, and nucleic acids are also players of extracellular vesicle surface interactions. Being secreted and present in high number in biological samples, collectively extracellular vesicles represent a uniquely large interactive surface area which can establish contacts both with cells and with molecules in the extracellular microenvironment. Here, we provide a brief overview of known components of the extracellular vesicle surface interactome and highlight some already established roles of the extracellular vesicle surface interactions in different biological processes in health and disease.

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Section: Evs and The Blood Plasmamentioning

confidence: 99%

Molecular interactions at the surface of extracellular vesicles

et al. 2018

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“…Several applications of confocal imaging of EV include the observation of vesicles inside the Golgi apparatus with a size of approximately 50–80 nm [87], and in addition, immobilization of EVs on a solid surface is used to directly visualize the individual vesicles [88], and also their localization in cells after uptake [89]. Cancer cells co‐cultured with EVs have been imaged with fluorescence microscopy [90], giving possible implications on uptake mechanisms.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence

Παναγοπούλου

Wark

Birch

et al. 2020

J of Extracellular Vesicle

109

105

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Extracellular vesicles (EVs) have numerous potential applications in the field of healthcare and diagnostics, and research into their biological functions is rapidly increasing. Mainly because of their small size and heterogeneity, there are significant challenges associated with their analysis and despite overt evidence of the potential of EVs in clinical diagnostic practice, guidelines for analytical procedures have not yet been properly established. Here, we present an overview of the main methods for studying the properties of EVs based on the principles of fluorescence. Setting aside the isolation, purification and physicochemical characterization strategies which answer questions about the size, surface charge and stability of EVs (reviewed elsewhere), we focus on available optical tools that enable the direct analysis of phenotype and mechanisms of interaction with tissues. In brief, the topics on which we elaborate range from the most popular approaches such as nanoparticle tracking analysis and flow cytometry, to less commonly used techniques such as fluorescence depolarization and microarrays as well as emerging areas such as fast fluorescence lifetime imaging microscopy (FLIM). We highlight that understanding the strengths and limitations of each method is essential for choosing the most appropriate combination of analytical tools. Finally, future directions of this rapidly developing area of medical diagnostics are discussed.

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“…39 Other antigenic methods have been recently proposed, such as the detection of TF þ MVs by scanning confocal microscopy. 115 Regarding functional tests, a first group of methods focuses on the PS-dependent procoagulant activity of MV:…”

Section: Methodological Considerationsmentioning

confidence: 99%

Microvesicles and Cancer Associated Thrombosis

Lacroix

Vallier

Bonifay

et al. 2019

Semin Thromb Hemost

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Microvesicles (MVs) are small membrane enclosed structures released into the extracellular space by virtually all cell types. Their composition varies according to the cell origin and the stimulus which caused their formation. They harbor functional molecules and participate in intercellular communication. Endothelium, inflammatory cells, and cancer cells produce procoagulant MVs which contribute to cancer-associated thrombosis (CAT) in animal models. The tissue factor (TF) conveyed by these MVs was shown to play a key role in different animal models of experimental CAT. Alternatively, other molecular mechanisms involving polyphosphates or phosphatidylethanolamine could also be involved. In clinical practice, an association between an increase in the number of TF-positive or the procoagulant activity of these MVs and the occurrence of CAT has indeed been demonstrated in pancreatic-biliary cancers, suggesting that they could behave as a biomarker predictive for CAT. However, to date, this association was not confirmed in other types of cancer. Potential causes explaining this limited associated between MVs and CAT are (1) the diversity of mechanisms associating MVs and different types of cancer; (2) a more complex role of MVs in hemostasis integrating their anticoagulant and fibrinolytic activity; and (3) the lack of sensitivity, reproducibility, and standardization of current methodologies permitting measurement of MVs. Each of these hypotheses constitutes an interesting exploration path for a future reassessment of the clinical interest of the MVs in CAT.

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Detection of tissue factor-positive extracellular vesicles by laser scanning confocal microscopy

Cited by 14 publications

References 34 publications

Molecular interactions at the surface of extracellular vesicles

Molecular interactions at the surface of extracellular vesicles

Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence

Microvesicles and Cancer Associated Thrombosis

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